Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06309 and H281DRAFT_06310 are separated by 16 nucleotides H281DRAFT_06310 and H281DRAFT_06311 are separated by 207 nucleotides H281DRAFT_06311 and H281DRAFT_06312 are separated by 19 nucleotides H281DRAFT_06312 and H281DRAFT_06313 are separated by 77 nucleotides
H281DRAFT_06309: H281DRAFT_06309 - ribonuclease T1, at 93,580 to 93,996
_06309
H281DRAFT_06310: H281DRAFT_06310 - Barstar (barnase inhibitor), at 94,013 to 94,573
_06310
H281DRAFT_06311: H281DRAFT_06311 - 16S rRNA (uracil1498-N3)-methyltransferase, at 94,781 to 95,521
_06311
H281DRAFT_06312: H281DRAFT_06312 - Uncharacterized conserved protein PhnB, glyoxalase superfamily, at 95,541 to 95,987
_06312
H281DRAFT_06313: H281DRAFT_06313 - spermidine synthase (EC 2.5.1.16), at 96,065 to 96,934
_06313
Position (kb)
94
95
96 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 93.784 kb on + strand at 93.865 kb on + strand, within H281DRAFT_06309 at 93.865 kb on + strand, within H281DRAFT_06309 at 93.865 kb on + strand, within H281DRAFT_06309 at 93.865 kb on + strand, within H281DRAFT_06309 at 93.866 kb on - strand, within H281DRAFT_06309 at 93.866 kb on - strand, within H281DRAFT_06309 at 93.892 kb on - strand, within H281DRAFT_06309 at 93.949 kb on + strand, within H281DRAFT_06309 at 93.953 kb on - strand, within H281DRAFT_06309 at 93.967 kb on + strand at 93.968 kb on - strand at 93.968 kb on - strand at 93.968 kb on - strand at 93.979 kb on - strand at 93.984 kb on + strand at 93.984 kb on + strand at 93.984 kb on + strand at 94.028 kb on + strand at 94.028 kb on + strand at 94.210 kb on - strand, within H281DRAFT_06310 at 94.210 kb on - strand, within H281DRAFT_06310 at 94.210 kb on - strand, within H281DRAFT_06310 at 94.281 kb on - strand, within H281DRAFT_06310 at 94.286 kb on + strand, within H281DRAFT_06310 at 94.287 kb on - strand, within H281DRAFT_06310 at 94.333 kb on + strand, within H281DRAFT_06310 at 94.333 kb on + strand, within H281DRAFT_06310 at 94.334 kb on - strand, within H281DRAFT_06310 at 94.371 kb on - strand, within H281DRAFT_06310 at 94.371 kb on - strand, within H281DRAFT_06310 at 94.371 kb on - strand at 94.408 kb on + strand, within H281DRAFT_06310 at 94.559 kb on + strand at 94.743 kb on - strand at 95.273 kb on + strand, within H281DRAFT_06311 at 95.273 kb on + strand at 95.274 kb on - strand, within H281DRAFT_06311 at 95.274 kb on - strand, within H281DRAFT_06311 at 95.274 kb on - strand, within H281DRAFT_06311 at 95.274 kb on - strand, within H281DRAFT_06311 at 95.274 kb on - strand, within H281DRAFT_06311 at 95.382 kb on + strand, within H281DRAFT_06311 at 95.382 kb on + strand, within H281DRAFT_06311 at 95.383 kb on - strand, within H281DRAFT_06311 at 95.383 kb on - strand, within H281DRAFT_06311 at 95.383 kb on - strand, within H281DRAFT_06311 at 95.411 kb on + strand, within H281DRAFT_06311 at 95.412 kb on - strand, within H281DRAFT_06311 at 95.530 kb on + strand at 95.531 kb on - strand at 95.537 kb on + strand at 95.538 kb on - strand at 95.538 kb on - strand at 95.692 kb on + strand, within H281DRAFT_06312 at 95.692 kb on + strand, within H281DRAFT_06312 at 95.692 kb on + strand, within H281DRAFT_06312 at 95.693 kb on - strand, within H281DRAFT_06312 at 95.693 kb on - strand, within H281DRAFT_06312 at 95.693 kb on - strand, within H281DRAFT_06312 at 95.693 kb on - strand, within H281DRAFT_06312 at 95.698 kb on + strand, within H281DRAFT_06312 at 95.698 kb on + strand, within H281DRAFT_06312 at 95.698 kb on + strand, within H281DRAFT_06312 at 95.698 kb on + strand, within H281DRAFT_06312 at 95.698 kb on + strand, within H281DRAFT_06312 at 95.698 kb on + strand, within H281DRAFT_06312 at 95.698 kb on + strand, within H281DRAFT_06312 at 95.698 kb on + strand, within H281DRAFT_06312 at 95.698 kb on + strand, within H281DRAFT_06312 at 95.698 kb on + strand, within H281DRAFT_06312 at 95.698 kb on + strand, within H281DRAFT_06312 at 95.698 kb on + strand, within H281DRAFT_06312 at 95.699 kb on - strand, within H281DRAFT_06312 at 95.699 kb on - strand, within H281DRAFT_06312 at 95.699 kb on - strand, within H281DRAFT_06312 at 95.699 kb on - strand, within H281DRAFT_06312 at 95.797 kb on + strand, within H281DRAFT_06312 at 95.797 kb on + strand, within H281DRAFT_06312 at 95.798 kb on - strand, within H281DRAFT_06312 at 95.798 kb on - strand, within H281DRAFT_06312 at 95.798 kb on - strand, within H281DRAFT_06312 at 95.798 kb on - strand, within H281DRAFT_06312 at 95.840 kb on + strand, within H281DRAFT_06312 at 95.872 kb on + strand, within H281DRAFT_06312 at 95.873 kb on - strand, within H281DRAFT_06312 at 95.970 kb on + strand at 96.027 kb on + strand at 96.092 kb on + strand at 96.092 kb on + strand at 96.093 kb on - strand at 96.097 kb on + strand at 96.150 kb on + strand at 96.150 kb on + strand at 96.150 kb on + strand at 96.151 kb on - strand at 96.264 kb on + strand, within H281DRAFT_06313 at 96.264 kb on + strand, within H281DRAFT_06313 at 96.265 kb on - strand, within H281DRAFT_06313 at 96.267 kb on - strand, within H281DRAFT_06313 at 96.267 kb on - strand, within H281DRAFT_06313 at 96.354 kb on + strand, within H281DRAFT_06313 at 96.355 kb on - strand, within H281DRAFT_06313 at 96.417 kb on + strand, within H281DRAFT_06313 at 96.417 kb on + strand, within H281DRAFT_06313 at 96.418 kb on - strand, within H281DRAFT_06313 at 96.418 kb on - strand, within H281DRAFT_06313 at 96.418 kb on - strand, within H281DRAFT_06313 at 96.433 kb on - strand, within H281DRAFT_06313 at 96.473 kb on + strand, within H281DRAFT_06313 at 96.473 kb on + strand, within H281DRAFT_06313 at 96.473 kb on + strand, within H281DRAFT_06313 at 96.486 kb on + strand, within H281DRAFT_06313 at 96.487 kb on - strand, within H281DRAFT_06313 at 96.487 kb on - strand, within H281DRAFT_06313
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 93,784 + +1.0 93,865 + H281DRAFT_06309 0.68 -1.2 93,865 + H281DRAFT_06309 0.68 -0.4 93,865 + H281DRAFT_06309 0.68 -0.3 93,865 + H281DRAFT_06309 0.68 +0.6 93,866 - H281DRAFT_06309 0.69 -0.0 93,866 - H281DRAFT_06309 0.69 +0.4 93,892 - H281DRAFT_06309 0.75 -0.2 93,949 + H281DRAFT_06309 0.88 -0.2 93,953 - H281DRAFT_06309 0.89 -1.0 93,967 + +0.6 93,968 - -1.2 93,968 - +0.1 93,968 - +0.2 93,979 - -0.6 93,984 + -0.5 93,984 + -0.5 93,984 + -2.7 94,028 + +0.2 94,028 + -0.5 94,210 - H281DRAFT_06310 0.35 -0.8 94,210 - H281DRAFT_06310 0.35 -0.1 94,210 - H281DRAFT_06310 0.35 -0.0 94,281 - H281DRAFT_06310 0.48 -0.3 94,286 + H281DRAFT_06310 0.49 -1.9 94,287 - H281DRAFT_06310 0.49 +1.4 94,333 + H281DRAFT_06310 0.57 -1.0 94,333 + H281DRAFT_06310 0.57 -1.5 94,334 - H281DRAFT_06310 0.57 -0.3 94,371 - H281DRAFT_06310 0.64 +0.2 94,371 - H281DRAFT_06310 0.64 +1.0 94,371 - +0.5 94,408 + H281DRAFT_06310 0.70 +0.1 94,559 + -0.6 94,743 - +0.2 95,273 + H281DRAFT_06311 0.66 +0.0 95,273 + +0.6 95,274 - H281DRAFT_06311 0.67 +0.1 95,274 - H281DRAFT_06311 0.67 +0.3 95,274 - H281DRAFT_06311 0.67 +2.0 95,274 - H281DRAFT_06311 0.67 -0.6 95,274 - H281DRAFT_06311 0.67 +1.3 95,382 + H281DRAFT_06311 0.81 +0.2 95,382 + H281DRAFT_06311 0.81 +0.4 95,383 - H281DRAFT_06311 0.81 +1.5 95,383 - H281DRAFT_06311 0.81 -0.2 95,383 - H281DRAFT_06311 0.81 +0.1 95,411 + H281DRAFT_06311 0.85 +0.3 95,412 - H281DRAFT_06311 0.85 +0.1 95,530 + +0.4 95,531 - +0.5 95,537 + -1.5 95,538 - +0.2 95,538 - +2.0 95,692 + H281DRAFT_06312 0.34 +0.4 95,692 + H281DRAFT_06312 0.34 +1.1 95,692 + H281DRAFT_06312 0.34 -0.2 95,693 - H281DRAFT_06312 0.34 +2.2 95,693 - H281DRAFT_06312 0.34 +0.2 95,693 - H281DRAFT_06312 0.34 +0.3 95,693 - H281DRAFT_06312 0.34 -0.1 95,698 + H281DRAFT_06312 0.35 +0.8 95,698 + H281DRAFT_06312 0.35 -0.5 95,698 + H281DRAFT_06312 0.35 -1.3 95,698 + H281DRAFT_06312 0.35 -0.7 95,698 + H281DRAFT_06312 0.35 +1.0 95,698 + H281DRAFT_06312 0.35 +3.0 95,698 + H281DRAFT_06312 0.35 +0.6 95,698 + H281DRAFT_06312 0.35 +0.4 95,698 + H281DRAFT_06312 0.35 -0.5 95,698 + H281DRAFT_06312 0.35 +0.2 95,698 + H281DRAFT_06312 0.35 +0.8 95,698 + H281DRAFT_06312 0.35 -0.3 95,699 - H281DRAFT_06312 0.35 +0.9 95,699 - H281DRAFT_06312 0.35 +0.2 95,699 - H281DRAFT_06312 0.35 +0.2 95,699 - H281DRAFT_06312 0.35 +0.5 95,797 + H281DRAFT_06312 0.57 -0.2 95,797 + H281DRAFT_06312 0.57 +0.1 95,798 - H281DRAFT_06312 0.57 +0.6 95,798 - H281DRAFT_06312 0.57 -0.2 95,798 - H281DRAFT_06312 0.57 +0.6 95,798 - H281DRAFT_06312 0.57 +0.7 95,840 + H281DRAFT_06312 0.67 -1.0 95,872 + H281DRAFT_06312 0.74 +0.5 95,873 - H281DRAFT_06312 0.74 -0.1 95,970 + -0.4 96,027 + +0.6 96,092 + +0.3 96,092 + +0.2 96,093 - -0.6 96,097 + +1.3 96,150 + -0.4 96,150 + +0.6 96,150 + -1.3 96,151 - -1.4 96,264 + H281DRAFT_06313 0.23 -0.5 96,264 + H281DRAFT_06313 0.23 -0.3 96,265 - H281DRAFT_06313 0.23 +0.2 96,267 - H281DRAFT_06313 0.23 -0.1 96,267 - H281DRAFT_06313 0.23 +1.3 96,354 + H281DRAFT_06313 0.33 +0.3 96,355 - H281DRAFT_06313 0.33 -0.5 96,417 + H281DRAFT_06313 0.40 +0.6 96,417 + H281DRAFT_06313 0.40 +0.8 96,418 - H281DRAFT_06313 0.41 +0.3 96,418 - H281DRAFT_06313 0.41 +0.2 96,418 - H281DRAFT_06313 0.41 -0.3 96,433 - H281DRAFT_06313 0.42 -0.5 96,473 + H281DRAFT_06313 0.47 -0.3 96,473 + H281DRAFT_06313 0.47 -0.2 96,473 + H281DRAFT_06313 0.47 +0.4 96,486 + H281DRAFT_06313 0.48 -0.5 96,487 - H281DRAFT_06313 0.49 +0.6 96,487 - H281DRAFT_06313 0.49 -0.5
Or see this region's nucleotide sequence