Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05002 and H281DRAFT_05003 overlap by 4 nucleotides H281DRAFT_05003 and H281DRAFT_05004 overlap by 4 nucleotides H281DRAFT_05004 and H281DRAFT_05005 are separated by 54 nucleotides H281DRAFT_05005 and H281DRAFT_05006 are separated by 311 nucleotides
H281DRAFT_05002: H281DRAFT_05002 - type III secretion protein (HrpB4), at 188,037 to 188,852
_05002
H281DRAFT_05003: H281DRAFT_05003 - type III secretion apparatus lipoprotein, YscJ/HrcJ family, at 188,849 to 189,670
_05003
H281DRAFT_05004: H281DRAFT_05004 - type III secretion inner rod protein HrpB2, at 189,667 to 190,059
_05004
H281DRAFT_05005: H281DRAFT_05005 - type III secretion protein HrpB1, at 190,114 to 190,731
_05005
H281DRAFT_05006: H281DRAFT_05006 - type III secretion protein U, at 191,043 to 192,119
_05006
Position (kb) 189
190
191 Strain fitness (log2 ratio) -1
0
1
2 at 188.689 kb on + strand, within H281DRAFT_05002 at 188.690 kb on - strand, within H281DRAFT_05002 at 188.694 kb on - strand, within H281DRAFT_05002 at 188.833 kb on + strand at 188.834 kb on - strand at 189.103 kb on + strand, within H281DRAFT_05003 at 189.104 kb on - strand, within H281DRAFT_05003 at 190.585 kb on - strand, within H281DRAFT_05005 at 190.673 kb on + strand at 190.674 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 188,689 + H281DRAFT_05002 0.80 +0.2 188,690 - H281DRAFT_05002 0.80 -1.0 188,694 - H281DRAFT_05002 0.81 -0.0 188,833 + +1.3 188,834 - +0.0 189,103 + H281DRAFT_05003 0.31 +0.3 189,104 - H281DRAFT_05003 0.31 +2.3 190,585 - H281DRAFT_05005 0.76 +0.5 190,673 + -0.1 190,674 - -0.8
Or see this region's nucleotide sequence