Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04949 and H281DRAFT_04950 are separated by 285 nucleotides H281DRAFT_04950 and H281DRAFT_04951 are separated by 60 nucleotides H281DRAFT_04951 and H281DRAFT_04952 overlap by 4 nucleotides H281DRAFT_04952 and H281DRAFT_04953 are separated by 137 nucleotides
H281DRAFT_04949: H281DRAFT_04949 - putative spermidine/putrescine transport system permease protein, at 133,872 to 134,699
_04949
H281DRAFT_04950: H281DRAFT_04950 - tRNA_Ser_GGA, at 134,985 to 135,075
_04950
H281DRAFT_04951: H281DRAFT_04951 - cation/acetate symporter, at 135,136 to 137,154
_04951
H281DRAFT_04952: H281DRAFT_04952 - putative solute:sodium symporter small subunit, at 137,151 to 137,585
_04952
H281DRAFT_04953: H281DRAFT_04953 - acetyl-coenzyme A synthetase, at 137,723 to 139,705
_04953
Position (kb) 135
136
137
138 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 134.648 kb on + strand at 134.651 kb on + strand at 134.652 kb on - strand at 134.652 kb on - strand at 134.652 kb on - strand at 134.682 kb on - strand at 134.682 kb on - strand at 134.706 kb on - strand at 134.706 kb on - strand at 134.706 kb on - strand at 134.724 kb on + strand at 134.724 kb on + strand at 134.918 kb on - strand at 134.918 kb on - strand at 135.138 kb on - strand at 135.152 kb on + strand at 135.152 kb on + strand at 135.152 kb on + strand at 135.153 kb on - strand at 135.171 kb on + strand at 135.172 kb on - strand at 135.172 kb on - strand at 135.203 kb on - strand at 135.329 kb on + strand at 135.332 kb on + strand at 135.332 kb on + strand at 135.332 kb on + strand at 135.332 kb on + strand at 135.497 kb on + strand, within H281DRAFT_04951 at 135.497 kb on + strand, within H281DRAFT_04951 at 135.585 kb on - strand, within H281DRAFT_04951 at 135.585 kb on - strand, within H281DRAFT_04951 at 135.585 kb on - strand, within H281DRAFT_04951 at 135.587 kb on + strand, within H281DRAFT_04951 at 135.587 kb on + strand, within H281DRAFT_04951 at 135.587 kb on + strand, within H281DRAFT_04951 at 135.587 kb on + strand, within H281DRAFT_04951 at 135.918 kb on - strand, within H281DRAFT_04951 at 135.989 kb on + strand, within H281DRAFT_04951 at 135.989 kb on + strand, within H281DRAFT_04951 at 135.989 kb on + strand, within H281DRAFT_04951 at 135.989 kb on + strand, within H281DRAFT_04951 at 135.990 kb on - strand, within H281DRAFT_04951 at 136.002 kb on + strand, within H281DRAFT_04951 at 136.039 kb on + strand, within H281DRAFT_04951 at 136.040 kb on - strand, within H281DRAFT_04951 at 136.411 kb on - strand, within H281DRAFT_04951 at 136.431 kb on - strand, within H281DRAFT_04951 at 136.514 kb on + strand, within H281DRAFT_04951 at 136.515 kb on - strand, within H281DRAFT_04951 at 136.758 kb on - strand, within H281DRAFT_04951 at 136.859 kb on + strand, within H281DRAFT_04951 at 136.859 kb on + strand, within H281DRAFT_04951 at 136.859 kb on + strand, within H281DRAFT_04951 at 136.860 kb on - strand, within H281DRAFT_04951 at 136.860 kb on - strand, within H281DRAFT_04951 at 136.958 kb on + strand at 136.959 kb on - strand at 137.087 kb on + strand at 137.087 kb on + strand at 137.088 kb on - strand at 137.088 kb on - strand at 137.088 kb on - strand at 137.088 kb on - strand at 137.123 kb on + strand at 137.314 kb on + strand, within H281DRAFT_04952 at 137.315 kb on - strand, within H281DRAFT_04952 at 137.315 kb on - strand, within H281DRAFT_04952 at 137.315 kb on - strand, within H281DRAFT_04952 at 137.315 kb on - strand, within H281DRAFT_04952 at 137.315 kb on - strand, within H281DRAFT_04952 at 137.336 kb on - strand, within H281DRAFT_04952 at 137.336 kb on - strand, within H281DRAFT_04952 at 137.492 kb on - strand, within H281DRAFT_04952 at 137.592 kb on - strand at 137.592 kb on - strand at 137.596 kb on - strand at 137.596 kb on - strand at 137.596 kb on - strand at 137.698 kb on + strand at 137.699 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 134,648 + +0.7 134,651 + -0.0 134,652 - +0.4 134,652 - +0.9 134,652 - +0.2 134,682 - +0.3 134,682 - +1.7 134,706 - +1.2 134,706 - +0.2 134,706 - -0.1 134,724 + +0.4 134,724 + -0.1 134,918 - +0.8 134,918 - -0.4 135,138 - -0.5 135,152 + -0.5 135,152 + +0.0 135,152 + -0.3 135,153 - -1.0 135,171 + -1.5 135,172 - -0.0 135,172 - +0.3 135,203 - +0.9 135,329 + -0.4 135,332 + -1.5 135,332 + +0.4 135,332 + -0.2 135,332 + -0.4 135,497 + H281DRAFT_04951 0.18 +0.6 135,497 + H281DRAFT_04951 0.18 -0.3 135,585 - H281DRAFT_04951 0.22 -0.2 135,585 - H281DRAFT_04951 0.22 +0.8 135,585 - H281DRAFT_04951 0.22 +0.3 135,587 + H281DRAFT_04951 0.22 +0.1 135,587 + H281DRAFT_04951 0.22 +1.5 135,587 + H281DRAFT_04951 0.22 +0.5 135,587 + H281DRAFT_04951 0.22 -0.0 135,918 - H281DRAFT_04951 0.39 +0.8 135,989 + H281DRAFT_04951 0.42 -0.8 135,989 + H281DRAFT_04951 0.42 +0.3 135,989 + H281DRAFT_04951 0.42 -0.4 135,989 + H281DRAFT_04951 0.42 +1.0 135,990 - H281DRAFT_04951 0.42 +0.3 136,002 + H281DRAFT_04951 0.43 -0.5 136,039 + H281DRAFT_04951 0.45 -0.7 136,040 - H281DRAFT_04951 0.45 +0.4 136,411 - H281DRAFT_04951 0.63 -1.2 136,431 - H281DRAFT_04951 0.64 -0.1 136,514 + H281DRAFT_04951 0.68 +0.1 136,515 - H281DRAFT_04951 0.68 -0.0 136,758 - H281DRAFT_04951 0.80 +0.7 136,859 + H281DRAFT_04951 0.85 +0.4 136,859 + H281DRAFT_04951 0.85 -0.4 136,859 + H281DRAFT_04951 0.85 -0.5 136,860 - H281DRAFT_04951 0.85 -0.1 136,860 - H281DRAFT_04951 0.85 -3.2 136,958 + -0.9 136,959 - -0.7 137,087 + +0.9 137,087 + +0.1 137,088 - -0.3 137,088 - -0.2 137,088 - -1.4 137,088 - +0.5 137,123 + +0.0 137,314 + H281DRAFT_04952 0.37 -0.2 137,315 - H281DRAFT_04952 0.38 +0.1 137,315 - H281DRAFT_04952 0.38 -0.5 137,315 - H281DRAFT_04952 0.38 -0.8 137,315 - H281DRAFT_04952 0.38 +0.7 137,315 - H281DRAFT_04952 0.38 -1.1 137,336 - H281DRAFT_04952 0.43 +0.0 137,336 - H281DRAFT_04952 0.43 +0.3 137,492 - H281DRAFT_04952 0.78 +0.2 137,592 - +0.0 137,592 - +0.5 137,596 - +0.9 137,596 - +0.3 137,596 - -0.6 137,698 + +0.8 137,699 - -0.0
Or see this region's nucleotide sequence