Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_04777

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_04776 and H281DRAFT_04777 are separated by 5 nucleotidesH281DRAFT_04777 and H281DRAFT_04778 are separated by 25 nucleotides H281DRAFT_04776: H281DRAFT_04776 - GDPmannose 4,6-dehydratase, at 197,526 to 198,644 _04776 H281DRAFT_04777: H281DRAFT_04777 - GDP-L-fucose synthase, at 198,650 to 199,606 _04777 H281DRAFT_04778: H281DRAFT_04778 - Glycosyltransferase involved in cell wall bisynthesis, at 199,632 to 200,774 _04778 Position (kb) 198 199 200Strain fitness (log2 ratio) -1 0 1at 197.938 kb on - strand, within H281DRAFT_04776at 198.061 kb on - strand, within H281DRAFT_04776at 198.177 kb on + strand, within H281DRAFT_04776at 198.736 kb on + strandat 198.737 kb on - strandat 198.737 kb on - strandat 198.894 kb on - strand, within H281DRAFT_04777at 198.984 kb on - strand, within H281DRAFT_04777at 199.112 kb on + strand, within H281DRAFT_04777at 199.169 kb on + strand, within H281DRAFT_04777at 199.574 kb on - strandat 199.800 kb on + strand, within H281DRAFT_04778at 199.801 kb on - strand, within H281DRAFT_04778at 199.942 kb on - strand, within H281DRAFT_04778at 200.455 kb on - strand, within H281DRAFT_04778

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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197,938 - H281DRAFT_04776 0.37 -0.7
198,061 - H281DRAFT_04776 0.48 -0.2
198,177 + H281DRAFT_04776 0.58 -0.7
198,736 + -0.8
198,737 - +0.5
198,737 - +1.6
198,894 - H281DRAFT_04777 0.25 -0.5
198,984 - H281DRAFT_04777 0.35 -1.7
199,112 + H281DRAFT_04777 0.48 -1.3
199,169 + H281DRAFT_04777 0.54 +0.7
199,574 - +1.6
199,800 + H281DRAFT_04778 0.15 +0.4
199,801 - H281DRAFT_04778 0.15 -0.4
199,942 - H281DRAFT_04778 0.27 -0.6
200,455 - H281DRAFT_04778 0.72 +0.1

Or see this region's nucleotide sequence