Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04701 and H281DRAFT_04702 are separated by 25 nucleotides H281DRAFT_04702 and H281DRAFT_04703 are separated by 47 nucleotides H281DRAFT_04703 and H281DRAFT_04704 are separated by 29 nucleotides
H281DRAFT_04701: H281DRAFT_04701 - molecular chaperone DnaJ, at 113,530 to 114,672
_04701
H281DRAFT_04702: H281DRAFT_04702 - para-aminobenzoate synthetase / 4-amino-4-deoxychorismate lyase, at 114,698 to 116,587
_04702
H281DRAFT_04703: H281DRAFT_04703 - ketopantoate hydroxymethyltransferase, at 116,635 to 117,450
_04703
H281DRAFT_04704: H281DRAFT_04704 - Deoxyadenosine/deoxycytidine kinase, at 117,480 to 118,160
_04704
Position (kb)
114
115
116
117 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 114.680 kb on + strand at 114.681 kb on - strand at 114.681 kb on - strand at 114.681 kb on - strand at 114.681 kb on - strand at 114.723 kb on + strand at 114.724 kb on - strand at 114.779 kb on + strand at 114.780 kb on + strand at 114.780 kb on - strand at 114.780 kb on - strand at 115.157 kb on + strand, within H281DRAFT_04702 at 115.169 kb on + strand, within H281DRAFT_04702 at 115.169 kb on + strand, within H281DRAFT_04702 at 115.169 kb on + strand, within H281DRAFT_04702 at 115.169 kb on + strand, within H281DRAFT_04702 at 115.169 kb on + strand, within H281DRAFT_04702 at 115.170 kb on - strand, within H281DRAFT_04702 at 115.170 kb on - strand, within H281DRAFT_04702 at 115.170 kb on - strand, within H281DRAFT_04702 at 115.175 kb on + strand, within H281DRAFT_04702 at 115.195 kb on - strand, within H281DRAFT_04702 at 115.532 kb on + strand, within H281DRAFT_04702 at 115.700 kb on + strand, within H281DRAFT_04702 at 115.851 kb on + strand, within H281DRAFT_04702 at 115.852 kb on - strand, within H281DRAFT_04702 at 115.852 kb on - strand, within H281DRAFT_04702 at 115.964 kb on + strand, within H281DRAFT_04702 at 115.964 kb on + strand, within H281DRAFT_04702 at 115.964 kb on + strand, within H281DRAFT_04702 at 115.965 kb on - strand, within H281DRAFT_04702 at 116.038 kb on - strand, within H281DRAFT_04702 at 116.134 kb on - strand, within H281DRAFT_04702 at 116.250 kb on + strand, within H281DRAFT_04702 at 116.251 kb on - strand, within H281DRAFT_04702 at 116.251 kb on - strand, within H281DRAFT_04702 at 116.251 kb on - strand, within H281DRAFT_04702 at 116.329 kb on + strand, within H281DRAFT_04702 at 116.329 kb on + strand, within H281DRAFT_04702 at 116.330 kb on - strand, within H281DRAFT_04702 at 116.330 kb on - strand, within H281DRAFT_04702 at 116.330 kb on - strand, within H281DRAFT_04702 at 116.383 kb on - strand, within H281DRAFT_04702 at 116.476 kb on + strand at 116.476 kb on + strand at 116.477 kb on - strand at 116.517 kb on + strand at 116.518 kb on - strand at 116.518 kb on - strand at 116.518 kb on - strand at 116.518 kb on - strand at 116.688 kb on - strand at 117.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 114,680 + -0.6 114,681 - -0.3 114,681 - -0.1 114,681 - -1.0 114,681 - -0.8 114,723 + -1.0 114,724 - +0.7 114,779 + -0.2 114,780 + -0.8 114,780 - -0.9 114,780 - +0.3 115,157 + H281DRAFT_04702 0.24 +0.0 115,169 + H281DRAFT_04702 0.25 -0.1 115,169 + H281DRAFT_04702 0.25 +0.2 115,169 + H281DRAFT_04702 0.25 -0.8 115,169 + H281DRAFT_04702 0.25 +0.0 115,169 + H281DRAFT_04702 0.25 +0.5 115,170 - H281DRAFT_04702 0.25 -0.1 115,170 - H281DRAFT_04702 0.25 +0.1 115,170 - H281DRAFT_04702 0.25 +0.3 115,175 + H281DRAFT_04702 0.25 -0.4 115,195 - H281DRAFT_04702 0.26 -0.5 115,532 + H281DRAFT_04702 0.44 -1.1 115,700 + H281DRAFT_04702 0.53 +3.1 115,851 + H281DRAFT_04702 0.61 -0.5 115,852 - H281DRAFT_04702 0.61 -0.3 115,852 - H281DRAFT_04702 0.61 -0.0 115,964 + H281DRAFT_04702 0.67 +1.5 115,964 + H281DRAFT_04702 0.67 -0.5 115,964 + H281DRAFT_04702 0.67 -0.2 115,965 - H281DRAFT_04702 0.67 +0.8 116,038 - H281DRAFT_04702 0.71 +0.3 116,134 - H281DRAFT_04702 0.76 +0.9 116,250 + H281DRAFT_04702 0.82 -0.8 116,251 - H281DRAFT_04702 0.82 -0.3 116,251 - H281DRAFT_04702 0.82 -0.2 116,251 - H281DRAFT_04702 0.82 -0.5 116,329 + H281DRAFT_04702 0.86 -0.3 116,329 + H281DRAFT_04702 0.86 +0.7 116,330 - H281DRAFT_04702 0.86 -0.4 116,330 - H281DRAFT_04702 0.86 -1.5 116,330 - H281DRAFT_04702 0.86 -0.3 116,383 - H281DRAFT_04702 0.89 -0.1 116,476 + -0.5 116,476 + +0.3 116,477 - -2.2 116,517 + -0.1 116,518 - -0.8 116,518 - -0.1 116,518 - -0.1 116,518 - +0.8 116,688 - -0.8 117,444 - -1.0
Or see this region's nucleotide sequence