Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04318 and H281DRAFT_04319 are separated by 311 nucleotides H281DRAFT_04319 and H281DRAFT_04320 are separated by 81 nucleotides H281DRAFT_04320 and H281DRAFT_04321 are separated by 218 nucleotides
H281DRAFT_04318: H281DRAFT_04318 - ribosome-associated protein, at 37,019 to 37,237
_04318
H281DRAFT_04319: H281DRAFT_04319 - hypothetical protein, at 37,549 to 38,013
_04319
H281DRAFT_04320: H281DRAFT_04320 - hypothetical protein, at 38,095 to 38,376
_04320
H281DRAFT_04321: H281DRAFT_04321 - DNA helicase-2 / ATP-dependent DNA helicase PcrA, at 38,595 to 40,724
_04321
Position (kb)
38
39 Strain fitness (log2 ratio)
-1
0
1
2
3 at 37.203 kb on + strand, within H281DRAFT_04318 at 37.272 kb on + strand at 37.273 kb on - strand at 37.273 kb on - strand at 37.451 kb on + strand at 37.452 kb on - strand at 37.533 kb on + strand at 37.533 kb on + strand at 37.533 kb on + strand at 37.534 kb on - strand at 37.534 kb on - strand at 37.563 kb on + strand at 37.563 kb on + strand at 37.563 kb on + strand at 37.564 kb on - strand at 37.564 kb on - strand at 37.564 kb on - strand at 37.656 kb on + strand, within H281DRAFT_04319 at 37.657 kb on - strand, within H281DRAFT_04319 at 37.664 kb on + strand, within H281DRAFT_04319 at 37.664 kb on + strand, within H281DRAFT_04319 at 37.664 kb on + strand, within H281DRAFT_04319 at 37.665 kb on - strand, within H281DRAFT_04319 at 37.665 kb on - strand, within H281DRAFT_04319 at 37.665 kb on - strand, within H281DRAFT_04319 at 37.665 kb on - strand, within H281DRAFT_04319 at 37.782 kb on - strand, within H281DRAFT_04319 at 37.782 kb on - strand, within H281DRAFT_04319 at 37.885 kb on + strand, within H281DRAFT_04319 at 37.886 kb on - strand, within H281DRAFT_04319 at 37.886 kb on - strand, within H281DRAFT_04319 at 37.886 kb on - strand, within H281DRAFT_04319 at 37.886 kb on - strand, within H281DRAFT_04319 at 38.004 kb on + strand at 38.004 kb on + strand at 38.081 kb on + strand at 38.112 kb on + strand at 38.374 kb on + strand at 38.375 kb on - strand at 38.375 kb on - strand at 38.375 kb on - strand at 38.432 kb on + strand at 38.466 kb on + strand at 38.467 kb on - strand at 38.510 kb on + strand at 38.547 kb on + strand at 38.547 kb on + strand at 38.548 kb on - strand at 38.586 kb on + strand at 38.586 kb on + strand at 38.774 kb on - strand at 38.866 kb on + strand, within H281DRAFT_04321 at 38.867 kb on - strand, within H281DRAFT_04321 at 38.867 kb on - strand, within H281DRAFT_04321 at 38.870 kb on + strand, within H281DRAFT_04321 at 38.870 kb on + strand, within H281DRAFT_04321 at 38.870 kb on + strand, within H281DRAFT_04321 at 38.870 kb on + strand, within H281DRAFT_04321 at 38.870 kb on + strand, within H281DRAFT_04321 at 38.870 kb on + strand, within H281DRAFT_04321 at 38.870 kb on + strand, within H281DRAFT_04321 at 38.871 kb on - strand, within H281DRAFT_04321 at 38.871 kb on - strand, within H281DRAFT_04321 at 38.871 kb on - strand, within H281DRAFT_04321 at 38.939 kb on + strand, within H281DRAFT_04321 at 38.967 kb on + strand, within H281DRAFT_04321 at 38.968 kb on - strand, within H281DRAFT_04321 at 39.216 kb on + strand, within H281DRAFT_04321 at 39.270 kb on + strand, within H281DRAFT_04321 at 39.271 kb on - strand, within H281DRAFT_04321 at 39.348 kb on + strand, within H281DRAFT_04321 at 39.349 kb on - strand, within H281DRAFT_04321 at 39.349 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 37,203 + H281DRAFT_04318 0.84 +2.1 37,272 + -0.2 37,273 - +3.0 37,273 - -0.2 37,451 + +0.7 37,452 - +0.8 37,533 + -0.1 37,533 + -0.2 37,533 + +0.2 37,534 - -0.4 37,534 - +0.7 37,563 + +0.3 37,563 + +0.7 37,563 + +0.4 37,564 - +1.6 37,564 - -0.0 37,564 - +0.7 37,656 + H281DRAFT_04319 0.23 +0.3 37,657 - H281DRAFT_04319 0.23 +0.1 37,664 + H281DRAFT_04319 0.25 +0.5 37,664 + H281DRAFT_04319 0.25 +0.1 37,664 + H281DRAFT_04319 0.25 -0.9 37,665 - H281DRAFT_04319 0.25 +0.3 37,665 - H281DRAFT_04319 0.25 +0.3 37,665 - H281DRAFT_04319 0.25 +0.1 37,665 - H281DRAFT_04319 0.25 -0.0 37,782 - H281DRAFT_04319 0.50 +0.2 37,782 - H281DRAFT_04319 0.50 -0.5 37,885 + H281DRAFT_04319 0.72 -0.2 37,886 - H281DRAFT_04319 0.72 +1.0 37,886 - H281DRAFT_04319 0.72 +0.6 37,886 - H281DRAFT_04319 0.72 +1.0 37,886 - H281DRAFT_04319 0.72 -0.6 38,004 + +1.2 38,004 + +0.1 38,081 + -0.4 38,112 + +0.5 38,374 + -0.3 38,375 - +0.8 38,375 - +0.2 38,375 - +0.4 38,432 + +0.0 38,466 + +0.8 38,467 - +0.2 38,510 + -0.5 38,547 + -0.0 38,547 + -0.3 38,548 - +0.1 38,586 + +0.2 38,586 + +2.3 38,774 - +0.6 38,866 + H281DRAFT_04321 0.13 +0.1 38,867 - H281DRAFT_04321 0.13 +0.2 38,867 - H281DRAFT_04321 0.13 -1.0 38,870 + H281DRAFT_04321 0.13 +0.0 38,870 + H281DRAFT_04321 0.13 +0.2 38,870 + H281DRAFT_04321 0.13 -0.4 38,870 + H281DRAFT_04321 0.13 +0.5 38,870 + H281DRAFT_04321 0.13 -1.4 38,870 + H281DRAFT_04321 0.13 -0.1 38,870 + H281DRAFT_04321 0.13 +1.0 38,871 - H281DRAFT_04321 0.13 -0.8 38,871 - H281DRAFT_04321 0.13 +0.8 38,871 - H281DRAFT_04321 0.13 +0.5 38,939 + H281DRAFT_04321 0.16 +0.6 38,967 + H281DRAFT_04321 0.17 +2.7 38,968 - H281DRAFT_04321 0.18 +0.2 39,216 + H281DRAFT_04321 0.29 -0.3 39,270 + H281DRAFT_04321 0.32 -0.4 39,271 - H281DRAFT_04321 0.32 -0.2 39,348 + H281DRAFT_04321 0.35 +0.9 39,349 - H281DRAFT_04321 0.35 +0.0 39,349 - -0.3
Or see this region's nucleotide sequence