Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_03215

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_03214 and H281DRAFT_03215 are separated by 463 nucleotidesH281DRAFT_03215 and H281DRAFT_03216 overlap by 4 nucleotidesH281DRAFT_03216 and H281DRAFT_03217 are separated by 58 nucleotides H281DRAFT_03214: H281DRAFT_03214 - 4-hydroxyphenylpyruvate dioxygenase, at 260,641 to 262,524 _03214 H281DRAFT_03215: H281DRAFT_03215 - 3-dehydroquinate dehydratase, at 262,988 to 263,443 _03215 H281DRAFT_03216: H281DRAFT_03216 - shikimate dehydrogenase (EC 1.1.1.25), at 263,440 to 264,342 _03216 H281DRAFT_03217: H281DRAFT_03217 - MFS transporter, ACS family, D-galactonate transporter, at 264,401 to 265,768 _03217 Position (kb) 262 263 264Strain fitness (log2 ratio) -1 0 1 2at 262.061 kb on - strand, within H281DRAFT_03214at 262.231 kb on + strand, within H281DRAFT_03214at 262.318 kb on + strand, within H281DRAFT_03214at 262.413 kb on - strandat 262.480 kb on + strandat 262.699 kb on + strandat 262.896 kb on + strandat 262.946 kb on - strandat 263.004 kb on + strandat 263.226 kb on - strand, within H281DRAFT_03215at 263.294 kb on - strand, within H281DRAFT_03215at 263.336 kb on + strand, within H281DRAFT_03215at 263.337 kb on - strand, within H281DRAFT_03215at 263.388 kb on - strand, within H281DRAFT_03215at 263.677 kb on + strand, within H281DRAFT_03216at 263.678 kb on - strand, within H281DRAFT_03216at 263.694 kb on - strand, within H281DRAFT_03216at 263.694 kb on - strand, within H281DRAFT_03216at 263.699 kb on - strand, within H281DRAFT_03216at 264.170 kb on - strand, within H281DRAFT_03216at 264.310 kb on + strandat 264.311 kb on - strandat 264.393 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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262,061 - H281DRAFT_03214 0.75 +0.7
262,231 + H281DRAFT_03214 0.84 +0.6
262,318 + H281DRAFT_03214 0.89 +0.6
262,413 - +0.2
262,480 + -0.1
262,699 + +0.2
262,896 + -1.4
262,946 - +0.4
263,004 + -0.1
263,226 - H281DRAFT_03215 0.52 -0.0
263,294 - H281DRAFT_03215 0.67 +0.2
263,336 + H281DRAFT_03215 0.76 -0.6
263,337 - H281DRAFT_03215 0.77 -0.3
263,388 - H281DRAFT_03215 0.88 -0.8
263,677 + H281DRAFT_03216 0.26 +0.6
263,678 - H281DRAFT_03216 0.26 -1.1
263,694 - H281DRAFT_03216 0.28 -1.3
263,694 - H281DRAFT_03216 0.28 +1.6
263,699 - H281DRAFT_03216 0.29 +1.0
264,170 - H281DRAFT_03216 0.81 +0.1
264,310 + +2.1
264,311 - -0.0
264,393 - +0.5

Or see this region's nucleotide sequence