Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02456 and H281DRAFT_02457 are separated by 28 nucleotides H281DRAFT_02457 and H281DRAFT_02458 overlap by 4 nucleotides H281DRAFT_02458 and H281DRAFT_02459 overlap by 11 nucleotides H281DRAFT_02459 and H281DRAFT_02460 are separated by 64 nucleotides
H281DRAFT_02456: H281DRAFT_02456 - sarcosine oxidase subunit beta, at 21,856 to 23,100
_02456
H281DRAFT_02457: H281DRAFT_02457 - sarcosine oxidase subunit delta, at 23,129 to 23,443
_02457
H281DRAFT_02458: H281DRAFT_02458 - sarcosine oxidase subunit alpha, at 23,440 to 26,442
_02458
H281DRAFT_02459: H281DRAFT_02459 - sarcosine oxidase subunit gamma, at 26,432 to 27,088
_02459
H281DRAFT_02460: H281DRAFT_02460 - hypothetical protein, at 27,153 to 27,416
_02460
Position (kb) 23
24
25
26
27 Strain fitness (log2 ratio) -2
-1
0
1
2 at 22.569 kb on + strand, within H281DRAFT_02456 at 22.696 kb on + strand, within H281DRAFT_02456 at 22.697 kb on - strand, within H281DRAFT_02456 at 22.750 kb on + strand, within H281DRAFT_02456 at 22.924 kb on + strand, within H281DRAFT_02456 at 22.925 kb on - strand, within H281DRAFT_02456 at 22.984 kb on + strand at 23.051 kb on - strand at 23.051 kb on - strand at 23.051 kb on - strand at 23.051 kb on - strand at 23.253 kb on - strand, within H281DRAFT_02457 at 23.306 kb on - strand, within H281DRAFT_02457 at 23.531 kb on - strand at 23.815 kb on + strand, within H281DRAFT_02458 at 23.818 kb on + strand, within H281DRAFT_02458 at 24.388 kb on + strand, within H281DRAFT_02458 at 24.395 kb on + strand, within H281DRAFT_02458 at 24.395 kb on + strand, within H281DRAFT_02458 at 24.625 kb on + strand, within H281DRAFT_02458 at 24.625 kb on + strand, within H281DRAFT_02458 at 24.626 kb on - strand, within H281DRAFT_02458 at 24.747 kb on + strand, within H281DRAFT_02458 at 24.888 kb on + strand, within H281DRAFT_02458 at 25.076 kb on + strand, within H281DRAFT_02458 at 25.085 kb on - strand, within H281DRAFT_02458 at 25.349 kb on - strand, within H281DRAFT_02458 at 25.496 kb on - strand, within H281DRAFT_02458 at 25.609 kb on + strand, within H281DRAFT_02458 at 25.610 kb on - strand, within H281DRAFT_02458 at 26.018 kb on - strand, within H281DRAFT_02458 at 26.037 kb on + strand, within H281DRAFT_02458 at 26.275 kb on + strand at 26.275 kb on + strand at 26.276 kb on - strand at 26.276 kb on - strand at 26.854 kb on + strand, within H281DRAFT_02459 at 26.855 kb on - strand, within H281DRAFT_02459 at 27.096 kb on - strand at 27.282 kb on + strand, within H281DRAFT_02460 at 27.286 kb on + strand, within H281DRAFT_02460 at 27.287 kb on - strand, within H281DRAFT_02460 at 27.333 kb on + strand, within H281DRAFT_02460 at 27.334 kb on - strand, within H281DRAFT_02460 at 27.415 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 22,569 + H281DRAFT_02456 0.57 -0.3 22,696 + H281DRAFT_02456 0.67 -0.1 22,697 - H281DRAFT_02456 0.68 -0.4 22,750 + H281DRAFT_02456 0.72 -0.1 22,924 + H281DRAFT_02456 0.86 +0.8 22,925 - H281DRAFT_02456 0.86 -1.8 22,984 + -0.4 23,051 - +0.1 23,051 - -0.6 23,051 - -0.3 23,051 - +0.1 23,253 - H281DRAFT_02457 0.39 -0.1 23,306 - H281DRAFT_02457 0.56 +0.7 23,531 - +1.4 23,815 + H281DRAFT_02458 0.12 +2.2 23,818 + H281DRAFT_02458 0.13 +2.5 24,388 + H281DRAFT_02458 0.32 -0.9 24,395 + H281DRAFT_02458 0.32 -0.4 24,395 + H281DRAFT_02458 0.32 +0.3 24,625 + H281DRAFT_02458 0.39 +0.3 24,625 + H281DRAFT_02458 0.39 +0.2 24,626 - H281DRAFT_02458 0.39 -0.7 24,747 + H281DRAFT_02458 0.44 +0.4 24,888 + H281DRAFT_02458 0.48 +0.2 25,076 + H281DRAFT_02458 0.54 -0.1 25,085 - H281DRAFT_02458 0.55 -0.4 25,349 - H281DRAFT_02458 0.64 +0.1 25,496 - H281DRAFT_02458 0.68 -2.2 25,609 + H281DRAFT_02458 0.72 -1.5 25,610 - H281DRAFT_02458 0.72 -0.2 26,018 - H281DRAFT_02458 0.86 -0.8 26,037 + H281DRAFT_02458 0.86 +0.4 26,275 + +0.6 26,275 + -0.5 26,276 - +0.2 26,276 - +0.3 26,854 + H281DRAFT_02459 0.64 +0.2 26,855 - H281DRAFT_02459 0.64 +1.1 27,096 - +2.7 27,282 + H281DRAFT_02460 0.49 -0.2 27,286 + H281DRAFT_02460 0.50 -0.1 27,287 - H281DRAFT_02460 0.51 +1.2 27,333 + H281DRAFT_02460 0.68 -0.1 27,334 - H281DRAFT_02460 0.69 +0.3 27,415 - +0.4
Or see this region's nucleotide sequence