Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_02214

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_02213 and H281DRAFT_02214 are separated by 336 nucleotidesH281DRAFT_02214 and H281DRAFT_02215 overlap by 4 nucleotidesH281DRAFT_02215 and H281DRAFT_02216 are separated by 14 nucleotides H281DRAFT_02212: H281DRAFT_02212 - pyrimidine 5'-nucleotidase, at 355,124 to 355,927 _02212 H281DRAFT_02213: H281DRAFT_02213 - transcriptional regulator, TetR family, at 355,894 to 356,574 _02213 H281DRAFT_02214: H281DRAFT_02214 - homoserine O-acetyltransferase, at 356,911 to 358,056 _02214 H281DRAFT_02215: H281DRAFT_02215 - methionine biosynthesis protein MetW, at 358,053 to 358,661 _02215 H281DRAFT_02216: H281DRAFT_02216 - MFS transporter, PAT family, beta-lactamase induction signal transducer AmpG, at 358,676 to 360,070 _02216 Position (kb) 356 357 358 359Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 355.998 kb on + strandat 355.998 kb on + strand, within H281DRAFT_02213at 355.999 kb on - strand, within H281DRAFT_02213at 356.098 kb on + strand, within H281DRAFT_02213at 356.099 kb on - strand, within H281DRAFT_02213at 356.138 kb on + strand, within H281DRAFT_02213at 356.138 kb on + strand, within H281DRAFT_02213at 356.139 kb on - strand, within H281DRAFT_02213at 356.444 kb on - strand, within H281DRAFT_02213at 356.444 kb on - strand, within H281DRAFT_02213at 356.479 kb on + strand, within H281DRAFT_02213at 356.479 kb on + strand, within H281DRAFT_02213at 356.479 kb on + strand, within H281DRAFT_02213at 356.480 kb on - strand, within H281DRAFT_02213at 356.480 kb on - strand, within H281DRAFT_02213at 356.482 kb on - strand, within H281DRAFT_02213at 356.526 kb on + strandat 356.527 kb on - strandat 356.527 kb on - strandat 356.633 kb on + strandat 356.634 kb on - strandat 356.634 kb on - strandat 356.715 kb on + strandat 356.715 kb on + strandat 356.715 kb on + strandat 356.715 kb on + strandat 356.715 kb on + strandat 356.715 kb on + strandat 356.716 kb on - strandat 356.716 kb on - strandat 356.716 kb on - strandat 356.744 kb on + strandat 356.745 kb on - strandat 356.745 kb on - strandat 356.747 kb on + strandat 356.748 kb on - strandat 356.748 kb on - strandat 356.811 kb on - strandat 356.812 kb on + strandat 356.812 kb on + strandat 356.995 kb on + strandat 357.017 kb on - strandat 357.017 kb on - strandat 357.017 kb on - strandat 357.017 kb on - strandat 357.097 kb on + strand, within H281DRAFT_02214at 357.168 kb on + strand, within H281DRAFT_02214at 357.334 kb on - strand, within H281DRAFT_02214at 357.400 kb on + strand, within H281DRAFT_02214at 357.400 kb on + strand, within H281DRAFT_02214at 357.526 kb on + strand, within H281DRAFT_02214at 357.526 kb on + strand, within H281DRAFT_02214at 357.526 kb on + strand, within H281DRAFT_02214at 357.526 kb on + strand, within H281DRAFT_02214at 357.527 kb on - strand, within H281DRAFT_02214at 357.527 kb on - strand, within H281DRAFT_02214at 357.538 kb on - strand, within H281DRAFT_02214at 357.592 kb on + strand, within H281DRAFT_02214at 357.592 kb on + strand, within H281DRAFT_02214at 357.592 kb on + strand, within H281DRAFT_02214at 357.593 kb on - strandat 357.593 kb on - strand, within H281DRAFT_02214at 357.664 kb on + strand, within H281DRAFT_02214at 357.664 kb on + strand, within H281DRAFT_02214at 357.665 kb on - strand, within H281DRAFT_02214at 357.665 kb on - strand, within H281DRAFT_02214at 357.665 kb on - strand, within H281DRAFT_02214at 357.665 kb on - strand, within H281DRAFT_02214at 357.704 kb on - strand, within H281DRAFT_02214at 357.704 kb on - strandat 357.704 kb on - strand, within H281DRAFT_02214at 357.737 kb on - strand, within H281DRAFT_02214at 357.754 kb on + strand, within H281DRAFT_02214at 357.754 kb on + strand, within H281DRAFT_02214at 357.754 kb on + strand, within H281DRAFT_02214at 357.754 kb on + strand, within H281DRAFT_02214at 357.754 kb on + strand, within H281DRAFT_02214at 357.754 kb on + strand, within H281DRAFT_02214at 357.755 kb on - strand, within H281DRAFT_02214at 357.755 kb on - strand, within H281DRAFT_02214at 357.755 kb on - strand, within H281DRAFT_02214at 357.758 kb on + strand, within H281DRAFT_02214at 357.758 kb on + strand, within H281DRAFT_02214at 357.758 kb on + strand, within H281DRAFT_02214at 357.759 kb on - strand, within H281DRAFT_02214at 357.759 kb on - strand, within H281DRAFT_02214at 357.759 kb on - strand, within H281DRAFT_02214at 357.759 kb on - strand, within H281DRAFT_02214at 357.759 kb on - strand, within H281DRAFT_02214at 357.759 kb on - strand, within H281DRAFT_02214at 357.781 kb on + strand, within H281DRAFT_02214at 357.781 kb on + strand, within H281DRAFT_02214at 357.782 kb on - strand, within H281DRAFT_02214at 357.834 kb on + strand, within H281DRAFT_02214at 357.835 kb on - strand, within H281DRAFT_02214at 357.835 kb on - strand, within H281DRAFT_02214at 357.848 kb on - strand, within H281DRAFT_02214at 357.944 kb on - strandat 358.022 kb on - strandat 358.025 kb on - strandat 358.074 kb on + strandat 358.074 kb on + strandat 358.400 kb on + strand, within H281DRAFT_02215at 358.400 kb on + strand, within H281DRAFT_02215at 358.400 kb on + strand, within H281DRAFT_02215at 358.400 kb on + strand, within H281DRAFT_02215at 358.400 kb on + strand, within H281DRAFT_02215at 358.401 kb on - strand, within H281DRAFT_02215at 358.486 kb on - strand, within H281DRAFT_02215at 358.628 kb on + strandat 358.628 kb on + strandat 358.629 kb on - strandat 358.629 kb on - strandat 358.641 kb on + strandat 358.642 kb on - strandat 358.659 kb on + strandat 358.660 kb on - strandat 358.708 kb on + strandat 358.708 kb on + strandat 358.709 kb on - strandat 358.750 kb on + strandat 358.751 kb on - strandat 358.783 kb on + strandat 358.783 kb on + strandat 358.815 kb on - strandat 358.815 kb on - strandat 358.815 kb on - strandat 358.815 kb on - strandat 358.893 kb on - strand, within H281DRAFT_02216at 358.893 kb on - strand, within H281DRAFT_02216at 358.932 kb on - strand, within H281DRAFT_02216

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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355,998 + +2.2
355,998 + H281DRAFT_02213 0.15 +0.9
355,999 - H281DRAFT_02213 0.15 +0.4
356,098 + H281DRAFT_02213 0.30 -0.5
356,099 - H281DRAFT_02213 0.30 +1.1
356,138 + H281DRAFT_02213 0.36 +0.0
356,138 + H281DRAFT_02213 0.36 -0.0
356,139 - H281DRAFT_02213 0.36 +0.2
356,444 - H281DRAFT_02213 0.81 +0.2
356,444 - H281DRAFT_02213 0.81 -0.1
356,479 + H281DRAFT_02213 0.86 +1.2
356,479 + H281DRAFT_02213 0.86 -0.1
356,479 + H281DRAFT_02213 0.86 +0.2
356,480 - H281DRAFT_02213 0.86 -0.6
356,480 - H281DRAFT_02213 0.86 -1.8
356,482 - H281DRAFT_02213 0.86 -0.0
356,526 + +0.5
356,527 - -0.2
356,527 - -0.7
356,633 + +0.4
356,634 - -5.4
356,634 - -2.2
356,715 + +0.3
356,715 + -0.1
356,715 + +0.9
356,715 + +0.6
356,715 + -0.6
356,715 + +0.0
356,716 - -3.6
356,716 - -1.8
356,716 - -2.9
356,744 + +1.1
356,745 - -4.8
356,745 - -6.0
356,747 + +1.3
356,748 - -3.3
356,748 - -4.3
356,811 - -4.6
356,812 + +0.9
356,812 + +0.3
356,995 + -2.2
357,017 - -3.6
357,017 - -0.6
357,017 - -3.8
357,017 - -2.9
357,097 + H281DRAFT_02214 0.16 -3.0
357,168 + H281DRAFT_02214 0.22 -5.4
357,334 - H281DRAFT_02214 0.37 -5.5
357,400 + H281DRAFT_02214 0.43 -3.1
357,400 + H281DRAFT_02214 0.43 -4.5
357,526 + H281DRAFT_02214 0.54 -0.4
357,526 + H281DRAFT_02214 0.54 -0.6
357,526 + H281DRAFT_02214 0.54 -3.7
357,526 + H281DRAFT_02214 0.54 -2.1
357,527 - H281DRAFT_02214 0.54 -5.6
357,527 - H281DRAFT_02214 0.54 -4.5
357,538 - H281DRAFT_02214 0.55 -4.3
357,592 + H281DRAFT_02214 0.59 -3.8
357,592 + H281DRAFT_02214 0.59 -5.4
357,592 + H281DRAFT_02214 0.59 -4.5
357,593 - -0.1
357,593 - H281DRAFT_02214 0.60 -2.7
357,664 + H281DRAFT_02214 0.66 -3.1
357,664 + H281DRAFT_02214 0.66 -4.2
357,665 - H281DRAFT_02214 0.66 -3.6
357,665 - H281DRAFT_02214 0.66 -3.8
357,665 - H281DRAFT_02214 0.66 -4.3
357,665 - H281DRAFT_02214 0.66 -3.8
357,704 - H281DRAFT_02214 0.69 -3.8
357,704 - +0.9
357,704 - H281DRAFT_02214 0.69 -3.1
357,737 - H281DRAFT_02214 0.72 -3.7
357,754 + H281DRAFT_02214 0.74 -3.8
357,754 + H281DRAFT_02214 0.74 -1.8
357,754 + H281DRAFT_02214 0.74 -3.3
357,754 + H281DRAFT_02214 0.74 -4.1
357,754 + H281DRAFT_02214 0.74 -4.0
357,754 + H281DRAFT_02214 0.74 -3.1
357,755 - H281DRAFT_02214 0.74 -4.9
357,755 - H281DRAFT_02214 0.74 -2.1
357,755 - H281DRAFT_02214 0.74 -5.0
357,758 + H281DRAFT_02214 0.74 -3.1
357,758 + H281DRAFT_02214 0.74 -5.0
357,758 + H281DRAFT_02214 0.74 -3.9
357,759 - H281DRAFT_02214 0.74 -4.0
357,759 - H281DRAFT_02214 0.74 -2.4
357,759 - H281DRAFT_02214 0.74 -5.3
357,759 - H281DRAFT_02214 0.74 -3.5
357,759 - H281DRAFT_02214 0.74 -3.6
357,759 - H281DRAFT_02214 0.74 -2.9
357,781 + H281DRAFT_02214 0.76 -4.3
357,781 + H281DRAFT_02214 0.76 -3.6
357,782 - H281DRAFT_02214 0.76 -1.1
357,834 + H281DRAFT_02214 0.81 -3.1
357,835 - H281DRAFT_02214 0.81 -3.4
357,835 - H281DRAFT_02214 0.81 -3.7
357,848 - H281DRAFT_02214 0.82 -0.9
357,944 - -4.9
358,022 - -3.6
358,025 - -1.1
358,074 + -4.0
358,074 + -4.7
358,400 + H281DRAFT_02215 0.57 -1.1
358,400 + H281DRAFT_02215 0.57 -4.2
358,400 + H281DRAFT_02215 0.57 -2.1
358,400 + H281DRAFT_02215 0.57 -3.3
358,400 + H281DRAFT_02215 0.57 -4.4
358,401 - H281DRAFT_02215 0.57 -3.1
358,486 - H281DRAFT_02215 0.71 -2.7
358,628 + -4.6
358,628 + -1.7
358,629 - -2.5
358,629 - -0.6
358,641 + -3.3
358,642 - -4.9
358,659 + +0.1
358,660 - +0.1
358,708 + -2.9
358,708 + -0.5
358,709 - +0.4
358,750 + -0.4
358,751 - -2.1
358,783 + -2.2
358,783 + -1.4
358,815 - -1.2
358,815 - -1.5
358,815 - -1.9
358,815 - -1.7
358,893 - H281DRAFT_02216 0.16 -1.7
358,893 - H281DRAFT_02216 0.16 -0.4
358,932 - H281DRAFT_02216 0.18 -0.6

Or see this region's nucleotide sequence