Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02008 and H281DRAFT_02009 are separated by 80 nucleotides H281DRAFT_02009 and H281DRAFT_02010 are separated by 341 nucleotides H281DRAFT_02010 and H281DRAFT_02011 are separated by 165 nucleotides
H281DRAFT_02008: H281DRAFT_02008 - phosphocarrier protein, at 125,916 to 126,185
_02008
H281DRAFT_02009: H281DRAFT_02009 - PTS system, ascorbate-specific IIA component, at 126,266 to 126,754
_02009
H281DRAFT_02010: H281DRAFT_02010 - glutathione synthase, at 127,096 to 128,052
_02010
H281DRAFT_02011: H281DRAFT_02011 - glutamate--cysteine ligase, at 128,218 to 129,507
_02011
Position (kb) 127
128
129 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 126.100 kb on + strand, within H281DRAFT_02008 at 126.100 kb on + strand, within H281DRAFT_02008 at 126.100 kb on + strand, within H281DRAFT_02008 at 126.100 kb on + strand, within H281DRAFT_02008 at 126.101 kb on - strand, within H281DRAFT_02008 at 126.101 kb on - strand, within H281DRAFT_02008 at 126.101 kb on - strand, within H281DRAFT_02008 at 126.101 kb on - strand, within H281DRAFT_02008 at 126.101 kb on - strand, within H281DRAFT_02008 at 126.101 kb on - strand, within H281DRAFT_02008 at 126.164 kb on + strand at 126.206 kb on + strand at 126.206 kb on + strand at 126.207 kb on - strand at 126.207 kb on - strand at 126.240 kb on + strand at 126.241 kb on - strand at 126.241 kb on - strand at 126.241 kb on - strand at 126.244 kb on + strand at 126.245 kb on - strand at 126.245 kb on - strand at 126.245 kb on - strand at 126.245 kb on - strand at 126.245 kb on - strand at 126.684 kb on + strand, within H281DRAFT_02009 at 126.956 kb on + strand at 126.957 kb on - strand at 126.957 kb on - strand at 126.990 kb on + strand at 126.990 kb on + strand at 126.990 kb on + strand at 126.991 kb on - strand at 126.991 kb on - strand at 126.991 kb on - strand at 127.012 kb on - strand at 127.057 kb on + strand at 127.057 kb on + strand at 127.058 kb on - strand at 127.058 kb on - strand at 127.058 kb on - strand at 127.058 kb on - strand at 127.058 kb on - strand at 127.532 kb on - strand, within H281DRAFT_02010 at 127.757 kb on + strand, within H281DRAFT_02010 at 127.757 kb on + strand, within H281DRAFT_02010 at 127.757 kb on + strand, within H281DRAFT_02010 at 127.757 kb on + strand, within H281DRAFT_02010 at 127.757 kb on + strand, within H281DRAFT_02010 at 127.757 kb on + strand, within H281DRAFT_02010 at 127.758 kb on - strand, within H281DRAFT_02010 at 127.758 kb on - strand, within H281DRAFT_02010 at 127.758 kb on - strand, within H281DRAFT_02010 at 127.758 kb on - strand, within H281DRAFT_02010 at 127.849 kb on - strand, within H281DRAFT_02010 at 127.949 kb on + strand, within H281DRAFT_02010 at 127.949 kb on + strand, within H281DRAFT_02010 at 127.949 kb on + strand, within H281DRAFT_02010 at 127.949 kb on + strand, within H281DRAFT_02010 at 127.949 kb on + strand, within H281DRAFT_02010 at 127.949 kb on + strand, within H281DRAFT_02010 at 127.950 kb on - strand, within H281DRAFT_02010 at 127.950 kb on - strand, within H281DRAFT_02010 at 127.956 kb on + strand, within H281DRAFT_02010 at 127.957 kb on - strand at 127.957 kb on - strand at 127.957 kb on - strand at 127.957 kb on - strand at 127.957 kb on - strand at 127.995 kb on + strand at 127.995 kb on + strand at 127.996 kb on - strand at 127.996 kb on - strand at 128.006 kb on + strand at 128.006 kb on + strand at 128.007 kb on - strand at 128.007 kb on - strand at 128.022 kb on + strand at 128.094 kb on + strand at 128.095 kb on - strand at 128.095 kb on - strand at 128.095 kb on - strand at 128.095 kb on - strand at 128.219 kb on + strand at 128.219 kb on + strand at 128.220 kb on - strand at 128.297 kb on + strand at 128.297 kb on + strand at 128.297 kb on + strand at 128.298 kb on - strand at 128.298 kb on - strand at 128.298 kb on - strand at 128.298 kb on - strand at 128.298 kb on - strand at 128.298 kb on - strand at 128.298 kb on - strand at 128.298 kb on - strand at 128.301 kb on + strand at 128.301 kb on + strand at 128.301 kb on + strand at 128.301 kb on + strand at 128.301 kb on + strand at 128.301 kb on + strand at 128.301 kb on + strand at 128.302 kb on - strand at 128.302 kb on - strand at 128.302 kb on - strand at 128.302 kb on - strand at 128.302 kb on - strand at 128.302 kb on - strand at 128.302 kb on - strand at 128.302 kb on - strand at 128.302 kb on - strand at 128.303 kb on + strand at 128.303 kb on + strand at 128.303 kb on + strand at 128.303 kb on + strand at 128.303 kb on + strand at 128.303 kb on + strand at 128.303 kb on + strand at 128.303 kb on + strand at 128.304 kb on - strand at 128.304 kb on - strand at 128.304 kb on - strand at 128.304 kb on - strand at 128.304 kb on - strand at 128.304 kb on - strand at 128.304 kb on - strand at 128.439 kb on - strand, within H281DRAFT_02011 at 128.439 kb on - strand, within H281DRAFT_02011 at 128.469 kb on + strand, within H281DRAFT_02011 at 128.469 kb on + strand, within H281DRAFT_02011 at 128.469 kb on + strand, within H281DRAFT_02011 at 128.469 kb on + strand, within H281DRAFT_02011 at 128.470 kb on - strand, within H281DRAFT_02011 at 128.470 kb on - strand, within H281DRAFT_02011 at 128.470 kb on - strand, within H281DRAFT_02011 at 128.470 kb on - strand, within H281DRAFT_02011 at 128.470 kb on - strand, within H281DRAFT_02011 at 128.471 kb on + strand, within H281DRAFT_02011 at 128.471 kb on + strand, within H281DRAFT_02011 at 128.471 kb on + strand, within H281DRAFT_02011 at 128.471 kb on + strand, within H281DRAFT_02011 at 128.472 kb on - strand, within H281DRAFT_02011 at 128.516 kb on + strand, within H281DRAFT_02011 at 128.516 kb on + strand, within H281DRAFT_02011 at 128.517 kb on - strand, within H281DRAFT_02011 at 128.517 kb on - strand, within H281DRAFT_02011 at 128.645 kb on + strand, within H281DRAFT_02011 at 128.646 kb on - strand, within H281DRAFT_02011 at 128.646 kb on - strand, within H281DRAFT_02011 at 128.646 kb on - strand, within H281DRAFT_02011 at 128.646 kb on - strand, within H281DRAFT_02011 at 128.675 kb on + strand, within H281DRAFT_02011 at 128.676 kb on - strand, within H281DRAFT_02011 at 128.676 kb on - strand, within H281DRAFT_02011 at 128.676 kb on - strand, within H281DRAFT_02011 at 128.805 kb on - strand at 128.868 kb on - strand, within H281DRAFT_02011 at 128.930 kb on + strand, within H281DRAFT_02011 at 128.931 kb on - strand, within H281DRAFT_02011 at 128.931 kb on - strand, within H281DRAFT_02011
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 126,100 + H281DRAFT_02008 0.68 -3.3 126,100 + H281DRAFT_02008 0.68 -2.4 126,100 + H281DRAFT_02008 0.68 -1.8 126,100 + H281DRAFT_02008 0.68 +0.5 126,101 - H281DRAFT_02008 0.69 -5.5 126,101 - H281DRAFT_02008 0.69 -2.8 126,101 - H281DRAFT_02008 0.69 -2.6 126,101 - H281DRAFT_02008 0.69 -3.9 126,101 - H281DRAFT_02008 0.69 -3.3 126,101 - H281DRAFT_02008 0.69 -1.6 126,164 + -3.7 126,206 + -0.9 126,206 + -0.3 126,207 - -0.8 126,207 - -0.9 126,240 + -0.5 126,241 - +0.9 126,241 - +1.2 126,241 - +0.4 126,244 + -0.9 126,245 - +1.9 126,245 - -0.1 126,245 - +1.3 126,245 - +0.7 126,245 - +0.9 126,684 + H281DRAFT_02009 0.85 -2.1 126,956 + +0.6 126,957 - +1.0 126,957 - +0.9 126,990 + +0.9 126,990 + +0.1 126,990 + +1.2 126,991 - +0.3 126,991 - +0.5 126,991 - +0.2 127,012 - +0.7 127,057 + +0.5 127,057 + +0.2 127,058 - -0.9 127,058 - +0.8 127,058 - +0.5 127,058 - -0.0 127,058 - -0.3 127,532 - H281DRAFT_02010 0.46 -0.9 127,757 + H281DRAFT_02010 0.69 +0.6 127,757 + H281DRAFT_02010 0.69 -0.1 127,757 + H281DRAFT_02010 0.69 -2.8 127,757 + H281DRAFT_02010 0.69 -0.2 127,757 + H281DRAFT_02010 0.69 -0.5 127,757 + H281DRAFT_02010 0.69 -1.8 127,758 - H281DRAFT_02010 0.69 -0.1 127,758 - H281DRAFT_02010 0.69 -0.2 127,758 - H281DRAFT_02010 0.69 +0.2 127,758 - H281DRAFT_02010 0.69 +1.7 127,849 - H281DRAFT_02010 0.79 +0.2 127,949 + H281DRAFT_02010 0.89 +0.1 127,949 + H281DRAFT_02010 0.89 -0.1 127,949 + H281DRAFT_02010 0.89 -2.3 127,949 + H281DRAFT_02010 0.89 -0.6 127,949 + H281DRAFT_02010 0.89 +0.7 127,949 + H281DRAFT_02010 0.89 +0.0 127,950 - H281DRAFT_02010 0.89 +0.0 127,950 - H281DRAFT_02010 0.89 +0.2 127,956 + H281DRAFT_02010 0.90 -0.1 127,957 - -1.6 127,957 - -0.8 127,957 - +0.8 127,957 - +0.7 127,957 - -0.2 127,995 + -0.9 127,995 + -0.2 127,996 - +0.3 127,996 - -0.5 128,006 + +0.4 128,006 + -0.8 128,007 - -0.3 128,007 - -0.0 128,022 + +0.7 128,094 + -2.1 128,095 - -0.1 128,095 - -0.1 128,095 - -0.7 128,095 - +0.5 128,219 + -0.3 128,219 + -0.9 128,220 - -0.2 128,297 + -0.8 128,297 + +0.0 128,297 + +0.9 128,298 - -0.6 128,298 - +0.0 128,298 - -2.1 128,298 - -0.2 128,298 - +1.7 128,298 - -0.4 128,298 - -2.5 128,298 - -0.1 128,301 + +0.3 128,301 + -0.3 128,301 + -0.1 128,301 + -1.3 128,301 + +0.9 128,301 + -1.4 128,301 + -0.8 128,302 - -1.5 128,302 - +0.1 128,302 - -0.3 128,302 - -0.3 128,302 - +0.4 128,302 - +0.9 128,302 - -1.7 128,302 - -0.9 128,302 - +0.9 128,303 + -0.7 128,303 + -0.8 128,303 + -0.3 128,303 + -1.2 128,303 + -0.1 128,303 + -0.1 128,303 + -0.8 128,303 + -0.5 128,304 - +0.4 128,304 - +0.3 128,304 - +1.0 128,304 - -0.5 128,304 - -0.6 128,304 - +0.4 128,304 - -0.1 128,439 - H281DRAFT_02011 0.17 +0.8 128,439 - H281DRAFT_02011 0.17 -1.7 128,469 + H281DRAFT_02011 0.19 +1.0 128,469 + H281DRAFT_02011 0.19 -0.2 128,469 + H281DRAFT_02011 0.19 -0.0 128,469 + H281DRAFT_02011 0.19 +0.5 128,470 - H281DRAFT_02011 0.20 +0.4 128,470 - H281DRAFT_02011 0.20 +0.1 128,470 - H281DRAFT_02011 0.20 +0.2 128,470 - H281DRAFT_02011 0.20 -0.7 128,470 - H281DRAFT_02011 0.20 +0.1 128,471 + H281DRAFT_02011 0.20 +0.1 128,471 + H281DRAFT_02011 0.20 +0.2 128,471 + H281DRAFT_02011 0.20 -0.6 128,471 + H281DRAFT_02011 0.20 -0.3 128,472 - H281DRAFT_02011 0.20 -0.9 128,516 + H281DRAFT_02011 0.23 +0.1 128,516 + H281DRAFT_02011 0.23 -0.1 128,517 - H281DRAFT_02011 0.23 -0.1 128,517 - H281DRAFT_02011 0.23 -1.1 128,645 + H281DRAFT_02011 0.33 -1.3 128,646 - H281DRAFT_02011 0.33 -0.7 128,646 - H281DRAFT_02011 0.33 -0.3 128,646 - H281DRAFT_02011 0.33 +0.4 128,646 - H281DRAFT_02011 0.33 -0.0 128,675 + H281DRAFT_02011 0.35 +0.6 128,676 - H281DRAFT_02011 0.36 +0.7 128,676 - H281DRAFT_02011 0.36 -0.5 128,676 - H281DRAFT_02011 0.36 -0.6 128,805 - +0.9 128,868 - H281DRAFT_02011 0.50 -0.1 128,930 + H281DRAFT_02011 0.55 -0.8 128,931 - H281DRAFT_02011 0.55 +0.0 128,931 - H281DRAFT_02011 0.55 -1.3
Or see this region's nucleotide sequence