Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01996 and H281DRAFT_01997 are separated by 167 nucleotides H281DRAFT_01997 and H281DRAFT_01998 are separated by 18 nucleotides H281DRAFT_01998 and H281DRAFT_01999 overlap by 28 nucleotides H281DRAFT_01999 and H281DRAFT_02000 are separated by 114 nucleotides
H281DRAFT_01996: H281DRAFT_01996 - malonyl-CoA O-methyltransferase, at 114,892 to 115,857
_01996
H281DRAFT_01997: H281DRAFT_01997 - comF family protein, at 116,025 to 116,726
_01997
H281DRAFT_01998: H281DRAFT_01998 - tRNA (cytidine/uridine-2'-O-)-methyltransferase, at 116,745 to 117,215
_01998
H281DRAFT_01999: H281DRAFT_01999 - O-acetyl-ADP-ribose deacetylase (regulator of RNase III), contains Macro domain, at 117,188 to 117,736
_01999
H281DRAFT_02000: H281DRAFT_02000 - glycerol-3-phosphate dehydrogenase (NAD(P)+), at 117,851 to 118,849
_02000
Position (kb)
116
117
118 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 115.886 kb on + strand at 115.886 kb on + strand at 115.886 kb on + strand at 115.887 kb on - strand at 115.887 kb on - strand at 115.956 kb on + strand at 115.956 kb on + strand at 115.957 kb on - strand at 115.957 kb on - strand at 116.106 kb on + strand, within H281DRAFT_01997 at 116.184 kb on + strand, within H281DRAFT_01997 at 116.184 kb on + strand, within H281DRAFT_01997 at 116.184 kb on + strand, within H281DRAFT_01997 at 116.427 kb on + strand, within H281DRAFT_01997 at 116.428 kb on - strand, within H281DRAFT_01997 at 116.429 kb on + strand, within H281DRAFT_01997 at 116.429 kb on + strand, within H281DRAFT_01997 at 116.605 kb on + strand, within H281DRAFT_01997 at 116.605 kb on + strand, within H281DRAFT_01997 at 116.606 kb on - strand, within H281DRAFT_01997 at 116.606 kb on - strand, within H281DRAFT_01997 at 116.606 kb on - strand, within H281DRAFT_01997 at 116.606 kb on - strand, within H281DRAFT_01997 at 116.623 kb on + strand, within H281DRAFT_01997 at 116.623 kb on + strand, within H281DRAFT_01997 at 116.623 kb on + strand, within H281DRAFT_01997 at 116.623 kb on + strand, within H281DRAFT_01997 at 116.624 kb on - strand, within H281DRAFT_01997 at 116.624 kb on - strand, within H281DRAFT_01997 at 116.624 kb on - strand, within H281DRAFT_01997 at 116.624 kb on - strand, within H281DRAFT_01997 at 116.725 kb on - strand at 116.731 kb on + strand at 116.732 kb on - strand at 116.744 kb on + strand at 116.744 kb on + strand at 116.744 kb on + strand at 116.744 kb on + strand at 116.744 kb on + strand at 116.745 kb on - strand at 116.745 kb on - strand at 116.745 kb on - strand at 116.745 kb on - strand at 116.806 kb on + strand, within H281DRAFT_01998 at 116.895 kb on + strand, within H281DRAFT_01998 at 116.896 kb on - strand, within H281DRAFT_01998 at 116.905 kb on - strand, within H281DRAFT_01998 at 116.945 kb on - strand, within H281DRAFT_01998 at 117.508 kb on - strand, within H281DRAFT_01999 at 117.508 kb on - strand, within H281DRAFT_01999 at 117.649 kb on + strand, within H281DRAFT_01999 at 117.649 kb on + strand, within H281DRAFT_01999 at 117.649 kb on + strand, within H281DRAFT_01999 at 117.650 kb on - strand, within H281DRAFT_01999 at 117.725 kb on + strand at 117.725 kb on + strand at 117.725 kb on + strand at 117.725 kb on + strand at 117.725 kb on + strand at 117.725 kb on + strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.727 kb on + strand at 117.728 kb on - strand at 117.728 kb on - strand at 117.736 kb on + strand at 117.736 kb on + strand at 117.736 kb on + strand at 117.737 kb on - strand at 117.737 kb on - strand at 117.805 kb on + strand at 117.805 kb on + strand at 117.806 kb on - strand at 117.852 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 115,886 + -2.2 115,886 + -0.2 115,886 + -0.3 115,887 - -0.4 115,887 - +3.5 115,956 + +0.9 115,956 + +1.2 115,957 - -0.1 115,957 - +1.7 116,106 + H281DRAFT_01997 0.12 +1.0 116,184 + H281DRAFT_01997 0.23 +1.9 116,184 + H281DRAFT_01997 0.23 -0.1 116,184 + H281DRAFT_01997 0.23 +0.8 116,427 + H281DRAFT_01997 0.57 +1.0 116,428 - H281DRAFT_01997 0.57 +0.9 116,429 + H281DRAFT_01997 0.58 +0.1 116,429 + H281DRAFT_01997 0.58 +0.2 116,605 + H281DRAFT_01997 0.83 -0.3 116,605 + H281DRAFT_01997 0.83 -0.5 116,606 - H281DRAFT_01997 0.83 +0.9 116,606 - H281DRAFT_01997 0.83 +0.1 116,606 - H281DRAFT_01997 0.83 -0.1 116,606 - H281DRAFT_01997 0.83 +0.1 116,623 + H281DRAFT_01997 0.85 -0.3 116,623 + H281DRAFT_01997 0.85 -0.4 116,623 + H281DRAFT_01997 0.85 +0.2 116,623 + H281DRAFT_01997 0.85 +0.5 116,624 - H281DRAFT_01997 0.85 -0.2 116,624 - H281DRAFT_01997 0.85 +0.3 116,624 - H281DRAFT_01997 0.85 +0.3 116,624 - H281DRAFT_01997 0.85 +0.2 116,725 - +1.0 116,731 + +0.4 116,732 - -0.1 116,744 + +0.9 116,744 + +0.1 116,744 + -0.1 116,744 + -0.1 116,744 + +0.3 116,745 - +0.2 116,745 - +0.3 116,745 - -0.2 116,745 - -0.1 116,806 + H281DRAFT_01998 0.13 +0.3 116,895 + H281DRAFT_01998 0.32 -0.4 116,896 - H281DRAFT_01998 0.32 +0.1 116,905 - H281DRAFT_01998 0.34 +0.2 116,945 - H281DRAFT_01998 0.42 -0.5 117,508 - H281DRAFT_01999 0.58 +1.1 117,508 - H281DRAFT_01999 0.58 -0.1 117,649 + H281DRAFT_01999 0.84 +0.0 117,649 + H281DRAFT_01999 0.84 -0.1 117,649 + H281DRAFT_01999 0.84 +0.5 117,650 - H281DRAFT_01999 0.84 +0.2 117,725 + +0.2 117,725 + +0.5 117,725 + +0.1 117,725 + -0.4 117,725 + +0.5 117,725 + -0.2 117,726 - -2.4 117,726 - +0.1 117,726 - +0.0 117,726 - -1.0 117,726 - -0.2 117,726 - +0.5 117,726 - +0.7 117,726 - +0.1 117,726 - -0.8 117,726 - +0.3 117,727 + +1.4 117,728 - +0.2 117,728 - -0.0 117,736 + +0.8 117,736 + +0.2 117,736 + +0.0 117,737 - -0.5 117,737 - +0.8 117,805 + +1.3 117,805 + +0.6 117,806 - -0.4 117,852 + +0.7
Or see this region's nucleotide sequence