Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01343 and H281DRAFT_01344 are separated by 72 nucleotides H281DRAFT_01344 and H281DRAFT_01345 are separated by 28 nucleotides H281DRAFT_01345 and H281DRAFT_01346 are separated by 97 nucleotides
H281DRAFT_01343: H281DRAFT_01343 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit, at 183,959 to 185,992
_01343
H281DRAFT_01344: H281DRAFT_01344 - methylglutaconyl-CoA hydratase, at 186,065 to 186,850
_01344
H281DRAFT_01345: H281DRAFT_01345 - 3-methylcrotonyl-CoA carboxylase beta subunit, at 186,879 to 188,486
_01345
H281DRAFT_01346: H281DRAFT_01346 - isovaleryl-CoA dehydrogenase, at 188,584 to 189,765
_01346
Position (kb)
186
187
188
189 Strain fitness (log2 ratio)
-2
-1
0
1 at 186.843 kb on + strand at 186.905 kb on + strand at 186.906 kb on - strand at 187.010 kb on - strand at 187.012 kb on + strand at 187.033 kb on + strand at 187.034 kb on - strand at 187.222 kb on + strand, within H281DRAFT_01345 at 187.374 kb on + strand, within H281DRAFT_01345 at 187.487 kb on + strand, within H281DRAFT_01345 at 187.487 kb on + strand, within H281DRAFT_01345 at 187.619 kb on - strand, within H281DRAFT_01345 at 187.637 kb on - strand, within H281DRAFT_01345 at 187.772 kb on + strand, within H281DRAFT_01345 at 187.773 kb on - strand, within H281DRAFT_01345 at 187.773 kb on - strand, within H281DRAFT_01345 at 187.773 kb on - strand, within H281DRAFT_01345 at 187.773 kb on - strand, within H281DRAFT_01345 at 187.906 kb on + strand, within H281DRAFT_01345 at 187.984 kb on + strand, within H281DRAFT_01345 at 188.065 kb on + strand, within H281DRAFT_01345 at 188.065 kb on + strand, within H281DRAFT_01345 at 188.066 kb on - strand, within H281DRAFT_01345 at 188.137 kb on + strand, within H281DRAFT_01345 at 188.138 kb on - strand at 188.242 kb on + strand, within H281DRAFT_01345 at 188.242 kb on + strand, within H281DRAFT_01345 at 188.243 kb on - strand, within H281DRAFT_01345 at 188.585 kb on + strand at 188.658 kb on - strand at 188.769 kb on - strand, within H281DRAFT_01346 at 188.849 kb on + strand, within H281DRAFT_01346 at 189.204 kb on + strand, within H281DRAFT_01346 at 189.364 kb on - strand, within H281DRAFT_01346 at 189.419 kb on + strand, within H281DRAFT_01346 at 189.419 kb on + strand, within H281DRAFT_01346 at 189.470 kb on - strand, within H281DRAFT_01346
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 186,843 + -1.0 186,905 + -1.0 186,906 - +0.0 187,010 - -0.9 187,012 + -0.5 187,033 + -0.5 187,034 - +0.1 187,222 + H281DRAFT_01345 0.21 -0.4 187,374 + H281DRAFT_01345 0.31 -2.2 187,487 + H281DRAFT_01345 0.38 -0.8 187,487 + H281DRAFT_01345 0.38 +0.4 187,619 - H281DRAFT_01345 0.46 +1.0 187,637 - H281DRAFT_01345 0.47 -1.3 187,772 + H281DRAFT_01345 0.56 -1.8 187,773 - H281DRAFT_01345 0.56 -0.9 187,773 - H281DRAFT_01345 0.56 -0.5 187,773 - H281DRAFT_01345 0.56 -0.7 187,773 - H281DRAFT_01345 0.56 -1.4 187,906 + H281DRAFT_01345 0.64 -0.8 187,984 + H281DRAFT_01345 0.69 -1.0 188,065 + H281DRAFT_01345 0.74 -0.9 188,065 + H281DRAFT_01345 0.74 -1.0 188,066 - H281DRAFT_01345 0.74 +0.1 188,137 + H281DRAFT_01345 0.78 +0.9 188,138 - +0.5 188,242 + H281DRAFT_01345 0.85 +0.5 188,242 + H281DRAFT_01345 0.85 -1.9 188,243 - H281DRAFT_01345 0.85 -1.2 188,585 + -1.0 188,658 - -0.7 188,769 - H281DRAFT_01346 0.16 -0.3 188,849 + H281DRAFT_01346 0.22 -0.5 189,204 + H281DRAFT_01346 0.52 +0.2 189,364 - H281DRAFT_01346 0.66 +0.2 189,419 + H281DRAFT_01346 0.71 +0.0 189,419 + H281DRAFT_01346 0.71 -0.7 189,470 - H281DRAFT_01346 0.75 +0.8
Or see this region's nucleotide sequence