Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00582 and H281DRAFT_00583 are separated by 188 nucleotides H281DRAFT_00583 and H281DRAFT_00584 overlap by 4 nucleotides H281DRAFT_00584 and H281DRAFT_00585 overlap by 14 nucleotides
H281DRAFT_00582: H281DRAFT_00582 - Acyl-CoA-binding protein, at 649,954 to 650,223
_00582
H281DRAFT_00583: H281DRAFT_00583 - tRNA threonylcarbamoyladenosine biosynthesis protein TsaB, at 650,412 to 651,215
_00583
H281DRAFT_00584: H281DRAFT_00584 - [SSU ribosomal protein S18P]-alanine acetyltransferase, at 651,212 to 651,706
_00584
H281DRAFT_00585: H281DRAFT_00585 - DNA polymerase, at 651,693 to 652,901
_00585
Position (kb) 651
652 Strain fitness (log2 ratio) -1
0
1
2 at 650.248 kb on + strand at 650.248 kb on + strand at 650.265 kb on + strand at 650.265 kb on + strand at 650.265 kb on + strand at 650.266 kb on - strand at 650.298 kb on + strand at 650.299 kb on - strand at 650.299 kb on - strand at 650.399 kb on + strand at 650.399 kb on + strand at 650.400 kb on - strand at 650.400 kb on - strand at 650.400 kb on - strand at 650.404 kb on + strand at 650.404 kb on + strand at 651.239 kb on + strand at 651.239 kb on + strand at 651.350 kb on + strand, within H281DRAFT_00584 at 651.395 kb on + strand, within H281DRAFT_00584 at 651.508 kb on + strand, within H281DRAFT_00584 at 651.578 kb on + strand, within H281DRAFT_00584 at 651.621 kb on - strand, within H281DRAFT_00584
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 650,248 + -0.4 650,248 + +1.4 650,265 + -0.1 650,265 + +1.9 650,265 + +0.3 650,266 - +0.1 650,298 + +2.4 650,299 - -0.3 650,299 - +0.2 650,399 + -0.5 650,399 + +0.7 650,400 - +0.7 650,400 - +0.6 650,400 - -0.2 650,404 + +0.1 650,404 + -0.4 651,239 + +0.5 651,239 + +0.7 651,350 + H281DRAFT_00584 0.28 +0.0 651,395 + H281DRAFT_00584 0.37 +0.3 651,508 + H281DRAFT_00584 0.60 -0.4 651,578 + H281DRAFT_00584 0.74 +1.9 651,621 - H281DRAFT_00584 0.83 +0.7
Or see this region's nucleotide sequence