Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00143 and H281DRAFT_00144 overlap by 8 nucleotides H281DRAFT_00144 and H281DRAFT_00145 overlap by 1 nucleotides H281DRAFT_00145 and H281DRAFT_00146 are separated by 89 nucleotides
H281DRAFT_00143: H281DRAFT_00143 - L-threonine O-3-phosphate decarboxylase, at 148,417 to 149,523
_00143
H281DRAFT_00144: H281DRAFT_00144 - adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 149,516 to 150,451
_00144
H281DRAFT_00145: H281DRAFT_00145 - adenosylcobinamide kinase /adenosylcobinamide-phosphate guanylyltransferase, at 150,451 to 151,005
_00145
H281DRAFT_00146: H281DRAFT_00146 - adenosylcobyric acid synthase (glutamine-hydrolysing), at 151,095 to 152,609
_00146
Position (kb)
149
150
151 Strain fitness (log2 ratio)
-1
0
1
2 at 149.062 kb on + strand, within H281DRAFT_00143 at 149.062 kb on + strand, within H281DRAFT_00143 at 149.062 kb on + strand, within H281DRAFT_00143 at 149.062 kb on + strand, within H281DRAFT_00143 at 149.063 kb on - strand, within H281DRAFT_00143 at 149.124 kb on - strand, within H281DRAFT_00143 at 149.243 kb on + strand, within H281DRAFT_00143 at 149.581 kb on + strand at 149.582 kb on - strand at 149.582 kb on - strand at 149.582 kb on - strand at 149.674 kb on - strand, within H281DRAFT_00144 at 149.674 kb on - strand, within H281DRAFT_00144 at 150.322 kb on - strand, within H281DRAFT_00144 at 150.560 kb on + strand, within H281DRAFT_00145 at 150.715 kb on + strand, within H281DRAFT_00145 at 150.715 kb on + strand, within H281DRAFT_00145 at 150.715 kb on + strand, within H281DRAFT_00145 at 150.716 kb on - strand, within H281DRAFT_00145 at 150.716 kb on - strand, within H281DRAFT_00145 at 150.733 kb on + strand, within H281DRAFT_00145 at 150.733 kb on + strand, within H281DRAFT_00145 at 150.733 kb on + strand, within H281DRAFT_00145 at 150.733 kb on + strand, within H281DRAFT_00145 at 150.905 kb on + strand, within H281DRAFT_00145 at 151.017 kb on - strand at 151.050 kb on - strand at 151.070 kb on + strand at 151.127 kb on - strand at 151.184 kb on + strand at 151.202 kb on - strand at 151.444 kb on - strand, within H281DRAFT_00146
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 149,062 + H281DRAFT_00143 0.58 +0.7 149,062 + H281DRAFT_00143 0.58 +1.0 149,062 + H281DRAFT_00143 0.58 +0.8 149,062 + H281DRAFT_00143 0.58 +0.5 149,063 - H281DRAFT_00143 0.58 +2.1 149,124 - H281DRAFT_00143 0.64 -0.5 149,243 + H281DRAFT_00143 0.75 +1.3 149,581 + +0.5 149,582 - -1.4 149,582 - +0.1 149,582 - -0.1 149,674 - H281DRAFT_00144 0.17 +0.4 149,674 - H281DRAFT_00144 0.17 +1.1 150,322 - H281DRAFT_00144 0.86 -0.2 150,560 + H281DRAFT_00145 0.20 +0.5 150,715 + H281DRAFT_00145 0.48 +0.6 150,715 + H281DRAFT_00145 0.48 +0.7 150,715 + H281DRAFT_00145 0.48 +1.1 150,716 - H281DRAFT_00145 0.48 +0.9 150,716 - H281DRAFT_00145 0.48 +0.6 150,733 + H281DRAFT_00145 0.51 +2.1 150,733 + H281DRAFT_00145 0.51 +1.3 150,733 + H281DRAFT_00145 0.51 +0.6 150,733 + H281DRAFT_00145 0.51 +0.3 150,905 + H281DRAFT_00145 0.82 +0.0 151,017 - +0.1 151,050 - -0.4 151,070 + +1.4 151,127 - -0.4 151,184 + +0.2 151,202 - -0.1 151,444 - H281DRAFT_00146 0.23 +0.2
Or see this region's nucleotide sequence