Experiment: No stressor control+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0475 and Dsui_0476 are separated by 45 nucleotides Dsui_0476 and Dsui_0477 are separated by 115 nucleotides Dsui_0477 and Dsui_0478 are separated by 2 nucleotides Dsui_0478 and Dsui_0479 are separated by 39 nucleotides
Dsui_0475: Dsui_0475 - PilZ domain-containing protein, at 481,299 to 481,670
_0475
Dsui_0476: Dsui_0476 - organic radical activating enzyme, at 481,716 to 482,363
_0476
Dsui_0477: Dsui_0477 - tol-pal system protein YbgF, at 482,479 to 483,255
_0477
Dsui_0478: Dsui_0478 - peptidoglycan-associated lipoprotein, at 483,258 to 483,773
_0478
Dsui_0479: Dsui_0479 - tol-pal system beta propeller repeat protein TolB, at 483,813 to 485,096
_0479
Position (kb)
482
483
484 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 481.485 kb on + strand, within Dsui_0475 at 481.611 kb on + strand, within Dsui_0475 at 481.700 kb on + strand at 481.822 kb on - strand, within Dsui_0476 at 482.045 kb on + strand, within Dsui_0476 at 482.121 kb on + strand, within Dsui_0476 at 482.135 kb on - strand, within Dsui_0476 at 482.179 kb on + strand, within Dsui_0476 at 482.284 kb on + strand at 482.302 kb on - strand at 482.306 kb on + strand at 482.575 kb on - strand, within Dsui_0477 at 482.722 kb on - strand, within Dsui_0477 at 482.804 kb on + strand, within Dsui_0477 at 482.853 kb on + strand, within Dsui_0477 at 483.264 kb on - strand at 483.798 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction No stressor control+ 10 mM ClO4 and 20 mM lactate remove 481,485 + Dsui_0475 0.50 +0.9 481,611 + Dsui_0475 0.84 +1.3 481,700 + -0.4 481,822 - Dsui_0476 0.16 +0.3 482,045 + Dsui_0476 0.51 -0.4 482,121 + Dsui_0476 0.62 +0.3 482,135 - Dsui_0476 0.65 -0.8 482,179 + Dsui_0476 0.71 -0.3 482,284 + -0.6 482,302 - -0.2 482,306 + +1.4 482,575 - Dsui_0477 0.12 -3.8 482,722 - Dsui_0477 0.31 -0.8 482,804 + Dsui_0477 0.42 -3.1 482,853 + Dsui_0477 0.48 -4.1 483,264 - +1.3 483,798 - -2.0
Or see this region's nucleotide sequence