Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_884 and Ac3H11_885 are separated by 22 nucleotides Ac3H11_885 and Ac3H11_886 are separated by 11 nucleotides Ac3H11_886 and Ac3H11_887 are separated by 125 nucleotides
Ac3H11_884 - Export protein, at 168,004 to 168,984
_884
Ac3H11_885 - hypothetical protein, at 169,007 to 169,168
_885
Ac3H11_886 - hypothetical protein, at 169,180 to 169,728
_886
Ac3H11_887 - putative exported protein, at 169,854 to 170,822
_887
Position (kb)
169
170 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 168.014 kb on + strand at 168.014 kb on + strand at 168.015 kb on - strand at 168.015 kb on - strand at 168.187 kb on + strand, within Ac3H11_884 at 168.187 kb on + strand, within Ac3H11_884 at 168.187 kb on + strand, within Ac3H11_884 at 168.188 kb on - strand, within Ac3H11_884 at 168.188 kb on - strand, within Ac3H11_884 at 168.188 kb on - strand, within Ac3H11_884 at 168.250 kb on + strand, within Ac3H11_884 at 168.251 kb on - strand, within Ac3H11_884 at 168.251 kb on - strand, within Ac3H11_884 at 168.417 kb on + strand, within Ac3H11_884 at 168.417 kb on + strand, within Ac3H11_884 at 168.418 kb on - strand, within Ac3H11_884 at 168.436 kb on + strand, within Ac3H11_884 at 168.436 kb on + strand, within Ac3H11_884 at 168.436 kb on + strand, within Ac3H11_884 at 168.436 kb on + strand, within Ac3H11_884 at 168.436 kb on + strand, within Ac3H11_884 at 168.436 kb on + strand, within Ac3H11_884 at 168.437 kb on - strand, within Ac3H11_884 at 168.437 kb on - strand, within Ac3H11_884 at 168.437 kb on - strand, within Ac3H11_884 at 168.437 kb on - strand, within Ac3H11_884 at 168.437 kb on - strand, within Ac3H11_884 at 168.437 kb on - strand, within Ac3H11_884 at 168.437 kb on - strand, within Ac3H11_884 at 168.607 kb on + strand, within Ac3H11_884 at 168.607 kb on + strand, within Ac3H11_884 at 168.607 kb on + strand, within Ac3H11_884 at 168.607 kb on + strand, within Ac3H11_884 at 168.608 kb on - strand, within Ac3H11_884 at 168.608 kb on - strand, within Ac3H11_884 at 168.608 kb on - strand, within Ac3H11_884 at 168.608 kb on - strand, within Ac3H11_884 at 168.608 kb on - strand, within Ac3H11_884 at 168.608 kb on - strand, within Ac3H11_884 at 168.608 kb on - strand, within Ac3H11_884 at 168.608 kb on - strand, within Ac3H11_884 at 168.608 kb on - strand, within Ac3H11_884 at 168.608 kb on - strand, within Ac3H11_884 at 168.757 kb on + strand, within Ac3H11_884 at 168.757 kb on + strand, within Ac3H11_884 at 168.758 kb on - strand, within Ac3H11_884 at 168.758 kb on - strand, within Ac3H11_884 at 168.802 kb on + strand, within Ac3H11_884 at 168.803 kb on - strand, within Ac3H11_884 at 168.803 kb on - strand, within Ac3H11_884 at 169.444 kb on + strand, within Ac3H11_886 at 169.444 kb on + strand, within Ac3H11_886 at 169.444 kb on + strand, within Ac3H11_886 at 169.444 kb on + strand, within Ac3H11_886 at 169.445 kb on - strand, within Ac3H11_886 at 169.445 kb on - strand, within Ac3H11_886 at 169.445 kb on - strand, within Ac3H11_886 at 169.445 kb on - strand, within Ac3H11_886 at 169.447 kb on - strand, within Ac3H11_886 at 169.447 kb on - strand, within Ac3H11_886 at 169.474 kb on + strand, within Ac3H11_886 at 169.474 kb on + strand, within Ac3H11_886 at 169.474 kb on + strand, within Ac3H11_886 at 169.474 kb on + strand, within Ac3H11_886 at 169.474 kb on + strand, within Ac3H11_886 at 169.474 kb on + strand, within Ac3H11_886 at 169.474 kb on + strand, within Ac3H11_886 at 169.475 kb on - strand, within Ac3H11_886 at 169.475 kb on - strand, within Ac3H11_886 at 169.475 kb on - strand, within Ac3H11_886 at 169.475 kb on - strand, within Ac3H11_886 at 169.475 kb on - strand, within Ac3H11_886 at 169.475 kb on - strand, within Ac3H11_886 at 169.475 kb on - strand, within Ac3H11_886 at 169.475 kb on - strand, within Ac3H11_886 at 169.489 kb on + strand, within Ac3H11_886 at 169.489 kb on + strand, within Ac3H11_886 at 169.658 kb on - strand, within Ac3H11_886 at 169.866 kb on + strand at 169.866 kb on + strand at 169.880 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 168,014 + +0.2 168,014 + -2.8 168,015 - +0.1 168,015 - +0.2 168,187 + Ac3H11_884 0.19 -0.2 168,187 + Ac3H11_884 0.19 +1.4 168,187 + Ac3H11_884 0.19 +0.2 168,188 - Ac3H11_884 0.19 +0.6 168,188 - Ac3H11_884 0.19 +2.1 168,188 - Ac3H11_884 0.19 +1.2 168,250 + Ac3H11_884 0.25 -0.3 168,251 - Ac3H11_884 0.25 +1.5 168,251 - Ac3H11_884 0.25 +0.5 168,417 + Ac3H11_884 0.42 -0.3 168,417 + Ac3H11_884 0.42 +1.3 168,418 - Ac3H11_884 0.42 -2.8 168,436 + Ac3H11_884 0.44 +1.0 168,436 + Ac3H11_884 0.44 +1.1 168,436 + Ac3H11_884 0.44 +0.4 168,436 + Ac3H11_884 0.44 -0.2 168,436 + Ac3H11_884 0.44 +1.2 168,436 + Ac3H11_884 0.44 -1.2 168,437 - Ac3H11_884 0.44 -1.1 168,437 - Ac3H11_884 0.44 +1.3 168,437 - Ac3H11_884 0.44 -2.9 168,437 - Ac3H11_884 0.44 -2.4 168,437 - Ac3H11_884 0.44 +0.1 168,437 - Ac3H11_884 0.44 +0.1 168,437 - Ac3H11_884 0.44 +0.5 168,607 + Ac3H11_884 0.61 +0.5 168,607 + Ac3H11_884 0.61 -0.5 168,607 + Ac3H11_884 0.61 +1.0 168,607 + Ac3H11_884 0.61 +0.4 168,608 - Ac3H11_884 0.62 +1.9 168,608 - Ac3H11_884 0.62 -2.5 168,608 - Ac3H11_884 0.62 +0.1 168,608 - Ac3H11_884 0.62 -1.3 168,608 - Ac3H11_884 0.62 -0.4 168,608 - Ac3H11_884 0.62 -0.5 168,608 - Ac3H11_884 0.62 +1.1 168,608 - Ac3H11_884 0.62 +1.5 168,608 - Ac3H11_884 0.62 -0.2 168,608 - Ac3H11_884 0.62 -0.5 168,757 + Ac3H11_884 0.77 -0.5 168,757 + Ac3H11_884 0.77 -1.4 168,758 - Ac3H11_884 0.77 +1.2 168,758 - Ac3H11_884 0.77 -0.7 168,802 + Ac3H11_884 0.81 +0.4 168,803 - Ac3H11_884 0.81 -0.6 168,803 - Ac3H11_884 0.81 -0.9 169,444 + Ac3H11_886 0.48 +0.5 169,444 + Ac3H11_886 0.48 +1.0 169,444 + Ac3H11_886 0.48 +0.1 169,444 + Ac3H11_886 0.48 +2.7 169,445 - Ac3H11_886 0.48 -0.3 169,445 - Ac3H11_886 0.48 +0.4 169,445 - Ac3H11_886 0.48 +0.7 169,445 - Ac3H11_886 0.48 -0.7 169,447 - Ac3H11_886 0.49 -0.7 169,447 - Ac3H11_886 0.49 -0.5 169,474 + Ac3H11_886 0.54 +1.4 169,474 + Ac3H11_886 0.54 -0.3 169,474 + Ac3H11_886 0.54 +0.6 169,474 + Ac3H11_886 0.54 +1.3 169,474 + Ac3H11_886 0.54 +0.7 169,474 + Ac3H11_886 0.54 -0.8 169,474 + Ac3H11_886 0.54 -0.3 169,475 - Ac3H11_886 0.54 +0.6 169,475 - Ac3H11_886 0.54 +1.3 169,475 - Ac3H11_886 0.54 -0.0 169,475 - Ac3H11_886 0.54 +2.3 169,475 - Ac3H11_886 0.54 -0.0 169,475 - Ac3H11_886 0.54 -0.2 169,475 - Ac3H11_886 0.54 +0.8 169,475 - Ac3H11_886 0.54 -1.0 169,489 + Ac3H11_886 0.56 +0.5 169,489 + Ac3H11_886 0.56 -0.7 169,658 - Ac3H11_886 0.87 +0.9 169,866 + +0.6 169,866 + -0.6 169,880 + -1.7
Or see this region's nucleotide sequence