Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_621 and Ac3H11_622 are separated by 665 nucleotides Ac3H11_622 and Ac3H11_623 are separated by 97 nucleotides
Ac3H11_621 - Thioredoxin reductase (EC 1.8.1.9), at 60,051 to 60,944
_621
Ac3H11_622 - Transcriptional regulator, LysR family, at 61,610 to 62,554
_622
Ac3H11_623 - Permeases of the major facilitator superfamily, at 62,652 to 63,863
_623
Position (kb) 61
62
63 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 60.779 kb on + strand, within Ac3H11_621 at 60.840 kb on + strand, within Ac3H11_621 at 60.840 kb on + strand, within Ac3H11_621 at 60.841 kb on - strand, within Ac3H11_621 at 60.841 kb on - strand, within Ac3H11_621 at 60.932 kb on + strand at 60.987 kb on - strand at 60.987 kb on - strand at 61.042 kb on + strand at 61.042 kb on + strand at 61.042 kb on + strand at 61.042 kb on + strand at 61.043 kb on - strand at 61.046 kb on + strand at 61.046 kb on + strand at 61.046 kb on + strand at 61.046 kb on + strand at 61.046 kb on + strand at 61.046 kb on + strand at 61.046 kb on + strand at 61.046 kb on + strand at 61.046 kb on + strand at 61.047 kb on - strand at 61.047 kb on - strand at 61.047 kb on - strand at 61.047 kb on - strand at 61.047 kb on - strand at 61.047 kb on - strand at 61.047 kb on - strand at 61.050 kb on + strand at 61.050 kb on + strand at 61.050 kb on + strand at 61.050 kb on + strand at 61.050 kb on + strand at 61.050 kb on + strand at 61.050 kb on + strand at 61.050 kb on + strand at 61.050 kb on + strand at 61.051 kb on - strand at 61.051 kb on - strand at 61.051 kb on - strand at 61.051 kb on - strand at 61.051 kb on - strand at 61.051 kb on - strand at 61.051 kb on - strand at 61.084 kb on + strand at 61.084 kb on + strand at 61.084 kb on + strand at 61.085 kb on - strand at 61.092 kb on + strand at 61.092 kb on + strand at 61.093 kb on - strand at 61.093 kb on - strand at 61.093 kb on - strand at 61.179 kb on - strand at 61.179 kb on - strand at 61.179 kb on - strand at 61.345 kb on + strand at 61.351 kb on + strand at 61.351 kb on + strand at 61.351 kb on + strand at 61.351 kb on + strand at 61.352 kb on - strand at 61.369 kb on + strand at 61.369 kb on + strand at 61.369 kb on + strand at 61.369 kb on + strand at 61.369 kb on + strand at 61.370 kb on - strand at 61.370 kb on - strand at 61.433 kb on + strand at 61.434 kb on - strand at 61.456 kb on + strand at 61.457 kb on - strand at 61.520 kb on - strand at 61.528 kb on + strand at 61.528 kb on + strand at 61.528 kb on + strand at 61.528 kb on + strand at 61.528 kb on + strand at 61.529 kb on - strand at 61.529 kb on - strand at 61.529 kb on - strand at 61.529 kb on - strand at 61.529 kb on - strand at 61.542 kb on - strand at 61.542 kb on - strand at 61.542 kb on - strand at 61.599 kb on - strand at 61.599 kb on - strand at 61.599 kb on - strand at 61.690 kb on - strand at 61.702 kb on - strand at 61.707 kb on + strand, within Ac3H11_622 at 61.716 kb on + strand, within Ac3H11_622 at 61.716 kb on + strand, within Ac3H11_622 at 61.716 kb on + strand, within Ac3H11_622 at 61.716 kb on + strand, within Ac3H11_622 at 61.717 kb on - strand, within Ac3H11_622 at 61.717 kb on - strand, within Ac3H11_622 at 61.807 kb on + strand, within Ac3H11_622 at 61.807 kb on + strand, within Ac3H11_622 at 61.807 kb on + strand, within Ac3H11_622 at 61.808 kb on - strand, within Ac3H11_622 at 61.848 kb on + strand, within Ac3H11_622 at 61.936 kb on - strand, within Ac3H11_622 at 62.181 kb on + strand, within Ac3H11_622 at 62.181 kb on + strand, within Ac3H11_622 at 62.181 kb on + strand, within Ac3H11_622 at 62.182 kb on - strand, within Ac3H11_622 at 62.182 kb on - strand, within Ac3H11_622 at 62.182 kb on - strand, within Ac3H11_622 at 62.182 kb on - strand, within Ac3H11_622 at 62.182 kb on - strand, within Ac3H11_622 at 62.365 kb on + strand, within Ac3H11_622 at 62.365 kb on + strand, within Ac3H11_622 at 62.365 kb on + strand, within Ac3H11_622 at 62.365 kb on + strand, within Ac3H11_622 at 62.365 kb on + strand, within Ac3H11_622 at 62.365 kb on + strand, within Ac3H11_622 at 62.366 kb on - strand, within Ac3H11_622 at 62.366 kb on - strand, within Ac3H11_622 at 62.588 kb on + strand at 62.591 kb on + strand at 62.629 kb on - strand at 62.763 kb on + strand at 62.764 kb on - strand at 62.767 kb on - strand at 62.767 kb on - strand at 62.830 kb on + strand, within Ac3H11_623 at 62.830 kb on + strand, within Ac3H11_623 at 63.180 kb on + strand, within Ac3H11_623 at 63.180 kb on + strand, within Ac3H11_623 at 63.180 kb on + strand, within Ac3H11_623 at 63.180 kb on + strand, within Ac3H11_623 at 63.180 kb on + strand, within Ac3H11_623 at 63.180 kb on + strand, within Ac3H11_623 at 63.181 kb on - strand, within Ac3H11_623 at 63.181 kb on - strand, within Ac3H11_623 at 63.181 kb on - strand, within Ac3H11_623
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 60,779 + Ac3H11_621 0.81 -1.1 60,840 + Ac3H11_621 0.88 -0.4 60,840 + Ac3H11_621 0.88 +0.2 60,841 - Ac3H11_621 0.88 -0.1 60,841 - Ac3H11_621 0.88 +0.6 60,932 + -0.3 60,987 - +0.4 60,987 - +0.7 61,042 + -0.6 61,042 + +0.7 61,042 + +0.3 61,042 + +0.3 61,043 - -0.1 61,046 + -0.6 61,046 + -2.2 61,046 + +1.7 61,046 + +0.1 61,046 + -1.2 61,046 + +0.5 61,046 + +0.8 61,046 + +0.0 61,046 + -0.1 61,047 - -0.0 61,047 - +0.1 61,047 - +1.0 61,047 - -0.7 61,047 - +0.9 61,047 - -1.3 61,047 - -1.2 61,050 + +0.5 61,050 + +0.1 61,050 + -3.0 61,050 + -1.0 61,050 + +1.0 61,050 + -0.7 61,050 + -0.1 61,050 + -0.4 61,050 + +1.4 61,051 - +1.2 61,051 - +0.6 61,051 - +1.0 61,051 - -1.2 61,051 - +1.1 61,051 - +0.0 61,051 - -1.1 61,084 + +0.6 61,084 + +0.5 61,084 + +2.2 61,085 - +0.0 61,092 + -1.0 61,092 + -0.9 61,093 - -2.8 61,093 - +0.2 61,093 - -1.2 61,179 - -0.2 61,179 - +0.2 61,179 - +0.9 61,345 + -1.4 61,351 + -2.8 61,351 + +0.7 61,351 + -0.5 61,351 + +0.8 61,352 - -0.2 61,369 + +0.3 61,369 + -0.0 61,369 + +1.8 61,369 + +1.0 61,369 + +0.5 61,370 - +0.9 61,370 - -0.6 61,433 + -1.9 61,434 - +0.6 61,456 + -0.0 61,457 - +0.6 61,520 - -1.2 61,528 + +0.3 61,528 + +0.8 61,528 + -0.9 61,528 + -0.3 61,528 + +1.4 61,529 - -0.4 61,529 - -0.9 61,529 - -1.0 61,529 - -1.7 61,529 - +1.0 61,542 - -0.7 61,542 - +0.7 61,542 - -0.0 61,599 - +0.7 61,599 - -0.2 61,599 - -1.6 61,690 - +0.6 61,702 - +0.1 61,707 + Ac3H11_622 0.10 -0.6 61,716 + Ac3H11_622 0.11 +1.0 61,716 + Ac3H11_622 0.11 -0.1 61,716 + Ac3H11_622 0.11 +0.2 61,716 + Ac3H11_622 0.11 -0.4 61,717 - Ac3H11_622 0.11 +1.2 61,717 - Ac3H11_622 0.11 +0.2 61,807 + Ac3H11_622 0.21 -1.0 61,807 + Ac3H11_622 0.21 +0.3 61,807 + Ac3H11_622 0.21 -0.7 61,808 - Ac3H11_622 0.21 +0.5 61,848 + Ac3H11_622 0.25 -0.1 61,936 - Ac3H11_622 0.34 +0.2 62,181 + Ac3H11_622 0.60 -3.1 62,181 + Ac3H11_622 0.60 +1.3 62,181 + Ac3H11_622 0.60 +0.1 62,182 - Ac3H11_622 0.61 +0.0 62,182 - Ac3H11_622 0.61 +0.2 62,182 - Ac3H11_622 0.61 -1.1 62,182 - Ac3H11_622 0.61 +0.4 62,182 - Ac3H11_622 0.61 +0.6 62,365 + Ac3H11_622 0.80 -1.1 62,365 + Ac3H11_622 0.80 -1.5 62,365 + Ac3H11_622 0.80 +0.5 62,365 + Ac3H11_622 0.80 +1.2 62,365 + Ac3H11_622 0.80 +1.0 62,365 + Ac3H11_622 0.80 -0.6 62,366 - Ac3H11_622 0.80 +0.2 62,366 - Ac3H11_622 0.80 +1.2 62,588 + +0.6 62,591 + -3.8 62,629 - -2.4 62,763 + +0.3 62,764 - +1.5 62,767 - -0.1 62,767 - -0.5 62,830 + Ac3H11_623 0.15 +0.9 62,830 + Ac3H11_623 0.15 +0.5 63,180 + Ac3H11_623 0.44 +0.5 63,180 + Ac3H11_623 0.44 -0.8 63,180 + Ac3H11_623 0.44 -0.6 63,180 + Ac3H11_623 0.44 +2.4 63,180 + Ac3H11_623 0.44 +0.8 63,180 + Ac3H11_623 0.44 +0.9 63,181 - Ac3H11_623 0.44 -0.2 63,181 - Ac3H11_623 0.44 +1.6 63,181 - Ac3H11_623 0.44 -0.7
Or see this region's nucleotide sequence