Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_515 and Ac3H11_516 are separated by 72 nucleotides Ac3H11_516 and Ac3H11_517 are separated by 41 nucleotides
Ac3H11_515 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 216,327 to 218,090
_515
Ac3H11_516 - COGs COG3146, at 218,163 to 219,503
_516
Ac3H11_517 - NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase, at 219,545 to 221,203
_517
Position (kb)
218
219
220 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 217.376 kb on + strand, within Ac3H11_515 at 217.376 kb on + strand, within Ac3H11_515 at 217.376 kb on + strand, within Ac3H11_515 at 217.376 kb on + strand, within Ac3H11_515 at 217.377 kb on - strand, within Ac3H11_515 at 217.377 kb on - strand, within Ac3H11_515 at 217.985 kb on + strand at 218.096 kb on + strand at 218.096 kb on + strand at 218.097 kb on - strand at 218.097 kb on - strand at 218.209 kb on + strand at 218.210 kb on - strand at 218.373 kb on + strand, within Ac3H11_516 at 218.373 kb on + strand, within Ac3H11_516 at 218.373 kb on + strand, within Ac3H11_516 at 218.373 kb on + strand, within Ac3H11_516 at 218.373 kb on + strand, within Ac3H11_516 at 218.373 kb on + strand, within Ac3H11_516 at 218.374 kb on - strand, within Ac3H11_516 at 218.374 kb on - strand, within Ac3H11_516 at 218.374 kb on - strand, within Ac3H11_516 at 218.374 kb on - strand, within Ac3H11_516 at 218.374 kb on - strand, within Ac3H11_516 at 218.395 kb on + strand, within Ac3H11_516 at 218.395 kb on + strand, within Ac3H11_516 at 218.395 kb on + strand, within Ac3H11_516 at 218.396 kb on - strand, within Ac3H11_516 at 218.396 kb on - strand, within Ac3H11_516 at 218.398 kb on + strand, within Ac3H11_516 at 218.398 kb on + strand, within Ac3H11_516 at 218.398 kb on + strand, within Ac3H11_516 at 218.398 kb on + strand, within Ac3H11_516 at 218.398 kb on + strand, within Ac3H11_516 at 218.398 kb on + strand, within Ac3H11_516 at 218.399 kb on - strand, within Ac3H11_516 at 218.399 kb on - strand, within Ac3H11_516 at 218.399 kb on - strand, within Ac3H11_516 at 218.399 kb on - strand, within Ac3H11_516 at 218.399 kb on - strand, within Ac3H11_516 at 218.399 kb on - strand, within Ac3H11_516 at 218.641 kb on + strand, within Ac3H11_516 at 218.653 kb on + strand, within Ac3H11_516 at 219.154 kb on + strand, within Ac3H11_516 at 219.154 kb on + strand, within Ac3H11_516 at 219.157 kb on + strand, within Ac3H11_516 at 219.158 kb on - strand, within Ac3H11_516 at 219.158 kb on - strand, within Ac3H11_516 at 219.158 kb on - strand, within Ac3H11_516 at 219.178 kb on + strand, within Ac3H11_516 at 219.178 kb on + strand, within Ac3H11_516 at 219.179 kb on - strand, within Ac3H11_516 at 219.208 kb on + strand, within Ac3H11_516 at 219.208 kb on + strand, within Ac3H11_516 at 219.208 kb on + strand, within Ac3H11_516 at 219.209 kb on - strand, within Ac3H11_516 at 219.209 kb on - strand, within Ac3H11_516 at 219.209 kb on - strand, within Ac3H11_516 at 219.218 kb on - strand, within Ac3H11_516 at 219.235 kb on + strand, within Ac3H11_516 at 219.236 kb on - strand, within Ac3H11_516 at 219.236 kb on - strand, within Ac3H11_516 at 219.236 kb on - strand, within Ac3H11_516 at 219.236 kb on - strand, within Ac3H11_516 at 219.343 kb on + strand, within Ac3H11_516 at 219.343 kb on + strand, within Ac3H11_516 at 219.343 kb on + strand, within Ac3H11_516 at 219.343 kb on + strand, within Ac3H11_516 at 219.344 kb on - strand, within Ac3H11_516 at 219.344 kb on - strand, within Ac3H11_516 at 219.411 kb on + strand at 219.411 kb on + strand at 219.638 kb on + strand at 219.639 kb on - strand at 219.689 kb on + strand at 219.893 kb on + strand, within Ac3H11_517 at 219.893 kb on + strand, within Ac3H11_517 at 219.893 kb on + strand, within Ac3H11_517 at 219.894 kb on - strand, within Ac3H11_517 at 219.894 kb on - strand, within Ac3H11_517 at 219.894 kb on - strand, within Ac3H11_517 at 219.894 kb on - strand, within Ac3H11_517 at 219.894 kb on - strand, within Ac3H11_517 at 219.918 kb on - strand, within Ac3H11_517 at 219.942 kb on - strand, within Ac3H11_517 at 219.942 kb on - strand, within Ac3H11_517 at 220.167 kb on - strand, within Ac3H11_517 at 220.167 kb on - strand, within Ac3H11_517 at 220.391 kb on + strand, within Ac3H11_517 at 220.391 kb on + strand, within Ac3H11_517 at 220.391 kb on + strand, within Ac3H11_517 at 220.392 kb on - strand, within Ac3H11_517 at 220.392 kb on - strand, within Ac3H11_517 at 220.392 kb on - strand, within Ac3H11_517 at 220.473 kb on + strand, within Ac3H11_517 at 220.473 kb on + strand, within Ac3H11_517 at 220.474 kb on - strand, within Ac3H11_517 at 220.474 kb on - strand, within Ac3H11_517
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 217,376 + Ac3H11_515 0.59 +0.3 217,376 + Ac3H11_515 0.59 +0.3 217,376 + Ac3H11_515 0.59 +0.8 217,376 + Ac3H11_515 0.59 -0.4 217,377 - Ac3H11_515 0.60 +1.1 217,377 - Ac3H11_515 0.60 -2.4 217,985 + +1.0 218,096 + -0.4 218,096 + -1.0 218,097 - +0.2 218,097 - +0.1 218,209 + +1.6 218,210 - -2.3 218,373 + Ac3H11_516 0.16 +0.3 218,373 + Ac3H11_516 0.16 -0.2 218,373 + Ac3H11_516 0.16 -0.6 218,373 + Ac3H11_516 0.16 +1.2 218,373 + Ac3H11_516 0.16 +0.9 218,373 + Ac3H11_516 0.16 -0.3 218,374 - Ac3H11_516 0.16 -0.3 218,374 - Ac3H11_516 0.16 +0.2 218,374 - Ac3H11_516 0.16 +1.6 218,374 - Ac3H11_516 0.16 +2.4 218,374 - Ac3H11_516 0.16 +0.7 218,395 + Ac3H11_516 0.17 +0.1 218,395 + Ac3H11_516 0.17 +1.6 218,395 + Ac3H11_516 0.17 +0.3 218,396 - Ac3H11_516 0.17 +0.7 218,396 - Ac3H11_516 0.17 -0.4 218,398 + Ac3H11_516 0.18 -0.2 218,398 + Ac3H11_516 0.18 -1.7 218,398 + Ac3H11_516 0.18 +1.1 218,398 + Ac3H11_516 0.18 -0.3 218,398 + Ac3H11_516 0.18 +0.1 218,398 + Ac3H11_516 0.18 -1.0 218,399 - Ac3H11_516 0.18 -1.0 218,399 - Ac3H11_516 0.18 +0.5 218,399 - Ac3H11_516 0.18 +0.8 218,399 - Ac3H11_516 0.18 +0.3 218,399 - Ac3H11_516 0.18 +0.2 218,399 - Ac3H11_516 0.18 -0.7 218,641 + Ac3H11_516 0.36 -0.1 218,653 + Ac3H11_516 0.37 +0.6 219,154 + Ac3H11_516 0.74 -0.9 219,154 + Ac3H11_516 0.74 -0.0 219,157 + Ac3H11_516 0.74 -0.8 219,158 - Ac3H11_516 0.74 +0.3 219,158 - Ac3H11_516 0.74 +0.7 219,158 - Ac3H11_516 0.74 +0.9 219,178 + Ac3H11_516 0.76 +0.5 219,178 + Ac3H11_516 0.76 +0.1 219,179 - Ac3H11_516 0.76 -0.1 219,208 + Ac3H11_516 0.78 -0.8 219,208 + Ac3H11_516 0.78 +0.6 219,208 + Ac3H11_516 0.78 -1.3 219,209 - Ac3H11_516 0.78 +0.1 219,209 - Ac3H11_516 0.78 -2.4 219,209 - Ac3H11_516 0.78 -0.5 219,218 - Ac3H11_516 0.79 +0.3 219,235 + Ac3H11_516 0.80 +1.3 219,236 - Ac3H11_516 0.80 -0.2 219,236 - Ac3H11_516 0.80 +0.5 219,236 - Ac3H11_516 0.80 -0.0 219,236 - Ac3H11_516 0.80 -0.8 219,343 + Ac3H11_516 0.88 -0.0 219,343 + Ac3H11_516 0.88 +0.6 219,343 + Ac3H11_516 0.88 -1.5 219,343 + Ac3H11_516 0.88 -0.2 219,344 - Ac3H11_516 0.88 +1.3 219,344 - Ac3H11_516 0.88 -0.2 219,411 + +0.3 219,411 + -0.2 219,638 + -2.5 219,639 - +0.8 219,689 + +0.1 219,893 + Ac3H11_517 0.21 +0.2 219,893 + Ac3H11_517 0.21 +0.4 219,893 + Ac3H11_517 0.21 -1.2 219,894 - Ac3H11_517 0.21 +3.0 219,894 - Ac3H11_517 0.21 +0.1 219,894 - Ac3H11_517 0.21 +1.9 219,894 - Ac3H11_517 0.21 +0.6 219,894 - Ac3H11_517 0.21 +1.4 219,918 - Ac3H11_517 0.22 +0.4 219,942 - Ac3H11_517 0.24 +2.0 219,942 - Ac3H11_517 0.24 -2.6 220,167 - Ac3H11_517 0.37 +1.3 220,167 - Ac3H11_517 0.37 +4.5 220,391 + Ac3H11_517 0.51 +0.5 220,391 + Ac3H11_517 0.51 -0.3 220,391 + Ac3H11_517 0.51 +1.0 220,392 - Ac3H11_517 0.51 +0.6 220,392 - Ac3H11_517 0.51 +0.0 220,392 - Ac3H11_517 0.51 +2.1 220,473 + Ac3H11_517 0.56 +0.6 220,473 + Ac3H11_517 0.56 +0.5 220,474 - Ac3H11_517 0.56 -1.6 220,474 - Ac3H11_517 0.56 +1.2
Or see this region's nucleotide sequence