Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_4918 and Ac3H11_4919 are separated by 169 nucleotides Ac3H11_4919 and Ac3H11_4920 are separated by 70 nucleotides Ac3H11_4920 and Ac3H11_4921 overlap by 18 nucleotides Ac3H11_4921 and Ac3H11_4922 overlap by 4 nucleotides
Ac3H11_4918 - Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2), at 80,342 to 81,928
_4918
Ac3H11_4919 - hypothetical protein, at 82,098 to 82,628
_4919
Ac3H11_4920 - hypothetical protein, at 82,699 to 82,917
_4920
Ac3H11_4921 - mRNA 3-end processing factor, at 82,900 to 83,868
_4921
Ac3H11_4922 - ATP-dependent DNA ligase (EC 6.5.1.1) LigC, at 83,865 to 85,676
_4922
Position (kb) 82
83 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 81.763 kb on - strand, within Ac3H11_4918 at 81.950 kb on + strand at 81.951 kb on - strand at 81.965 kb on + strand at 81.965 kb on + strand at 81.965 kb on + strand at 81.965 kb on + strand at 81.966 kb on - strand at 82.068 kb on + strand at 82.068 kb on + strand at 82.069 kb on - strand at 82.069 kb on - strand at 82.312 kb on + strand, within Ac3H11_4919 at 82.312 kb on + strand, within Ac3H11_4919 at 82.312 kb on + strand, within Ac3H11_4919 at 82.345 kb on + strand, within Ac3H11_4919 at 82.345 kb on + strand, within Ac3H11_4919 at 82.346 kb on - strand, within Ac3H11_4919 at 82.346 kb on - strand, within Ac3H11_4919 at 82.346 kb on - strand, within Ac3H11_4919 at 82.377 kb on + strand, within Ac3H11_4919 at 82.378 kb on - strand, within Ac3H11_4919 at 82.444 kb on + strand, within Ac3H11_4919 at 82.445 kb on - strand, within Ac3H11_4919 at 82.681 kb on + strand at 82.682 kb on - strand at 82.701 kb on - strand at 82.701 kb on - strand at 82.701 kb on - strand at 82.755 kb on - strand, within Ac3H11_4920 at 82.789 kb on + strand, within Ac3H11_4920 at 82.790 kb on - strand, within Ac3H11_4920 at 82.790 kb on - strand, within Ac3H11_4920 at 82.846 kb on + strand, within Ac3H11_4920 at 82.847 kb on - strand, within Ac3H11_4920 at 82.847 kb on - strand, within Ac3H11_4920 at 82.847 kb on - strand, within Ac3H11_4920 at 82.867 kb on + strand, within Ac3H11_4920 at 82.867 kb on + strand, within Ac3H11_4920 at 82.867 kb on + strand, within Ac3H11_4920 at 82.868 kb on - strand, within Ac3H11_4920 at 82.868 kb on - strand, within Ac3H11_4920 at 82.948 kb on + strand at 82.948 kb on + strand at 82.948 kb on + strand at 82.949 kb on - strand at 83.023 kb on + strand, within Ac3H11_4921 at 83.023 kb on + strand, within Ac3H11_4921 at 83.143 kb on + strand, within Ac3H11_4921 at 83.143 kb on + strand, within Ac3H11_4921 at 83.143 kb on + strand, within Ac3H11_4921 at 83.143 kb on + strand, within Ac3H11_4921 at 83.144 kb on - strand, within Ac3H11_4921 at 83.144 kb on - strand, within Ac3H11_4921 at 83.234 kb on - strand, within Ac3H11_4921 at 83.234 kb on - strand, within Ac3H11_4921 at 83.320 kb on + strand, within Ac3H11_4921 at 83.320 kb on + strand, within Ac3H11_4921 at 83.320 kb on + strand, within Ac3H11_4921 at 83.320 kb on + strand, within Ac3H11_4921 at 83.321 kb on - strand, within Ac3H11_4921 at 83.327 kb on - strand, within Ac3H11_4921 at 83.636 kb on + strand, within Ac3H11_4921 at 83.636 kb on + strand, within Ac3H11_4921 at 83.636 kb on + strand, within Ac3H11_4921 at 83.637 kb on - strand, within Ac3H11_4921 at 83.637 kb on - strand, within Ac3H11_4921 at 83.637 kb on - strand, within Ac3H11_4921
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 81,763 - Ac3H11_4918 0.90 -0.9 81,950 + -2.5 81,951 - +0.4 81,965 + +0.2 81,965 + +0.6 81,965 + -2.9 81,965 + -2.7 81,966 - +0.5 82,068 + +1.6 82,068 + -2.3 82,069 - -2.1 82,069 - -2.8 82,312 + Ac3H11_4919 0.40 -1.3 82,312 + Ac3H11_4919 0.40 +1.0 82,312 + Ac3H11_4919 0.40 -0.2 82,345 + Ac3H11_4919 0.47 +0.5 82,345 + Ac3H11_4919 0.47 -0.5 82,346 - Ac3H11_4919 0.47 -1.4 82,346 - Ac3H11_4919 0.47 +0.0 82,346 - Ac3H11_4919 0.47 -1.0 82,377 + Ac3H11_4919 0.53 -0.8 82,378 - Ac3H11_4919 0.53 +0.0 82,444 + Ac3H11_4919 0.65 +0.7 82,445 - Ac3H11_4919 0.65 -1.3 82,681 + +0.3 82,682 - -2.7 82,701 - +1.6 82,701 - -1.0 82,701 - +0.6 82,755 - Ac3H11_4920 0.26 -1.8 82,789 + Ac3H11_4920 0.41 -0.2 82,790 - Ac3H11_4920 0.42 +0.5 82,790 - Ac3H11_4920 0.42 -0.6 82,846 + Ac3H11_4920 0.67 +1.0 82,847 - Ac3H11_4920 0.68 -1.4 82,847 - Ac3H11_4920 0.68 -0.2 82,847 - Ac3H11_4920 0.68 +1.5 82,867 + Ac3H11_4920 0.77 +1.3 82,867 + Ac3H11_4920 0.77 +0.6 82,867 + Ac3H11_4920 0.77 -2.0 82,868 - Ac3H11_4920 0.77 -1.0 82,868 - Ac3H11_4920 0.77 +0.8 82,948 + -1.8 82,948 + +0.5 82,948 + -0.7 82,949 - -0.3 83,023 + Ac3H11_4921 0.13 -3.1 83,023 + Ac3H11_4921 0.13 +1.1 83,143 + Ac3H11_4921 0.25 -0.8 83,143 + Ac3H11_4921 0.25 -1.2 83,143 + Ac3H11_4921 0.25 +0.5 83,143 + Ac3H11_4921 0.25 -0.5 83,144 - Ac3H11_4921 0.25 +0.4 83,144 - Ac3H11_4921 0.25 +1.5 83,234 - Ac3H11_4921 0.34 -0.4 83,234 - Ac3H11_4921 0.34 +0.5 83,320 + Ac3H11_4921 0.43 -1.8 83,320 + Ac3H11_4921 0.43 -2.9 83,320 + Ac3H11_4921 0.43 -2.0 83,320 + Ac3H11_4921 0.43 -2.3 83,321 - Ac3H11_4921 0.43 -1.3 83,327 - Ac3H11_4921 0.44 +1.4 83,636 + Ac3H11_4921 0.76 -2.2 83,636 + Ac3H11_4921 0.76 -0.2 83,636 + Ac3H11_4921 0.76 -0.1 83,637 - Ac3H11_4921 0.76 +0.0 83,637 - Ac3H11_4921 0.76 +0.8 83,637 - Ac3H11_4921 0.76 +0.2
Or see this region's nucleotide sequence