Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1481 and Ac3H11_1482 are separated by 138 nucleotides Ac3H11_1482 and Ac3H11_1483 are separated by 10 nucleotides Ac3H11_1483 and Ac3H11_1484 are separated by 70 nucleotides
Ac3H11_1481 - putative exported protein, at 60,927 to 61,907
_1481
Ac3H11_1482 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 62,046 to 62,870
_1482
Ac3H11_1483 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 62,881 to 63,630
_1483
Ac3H11_1484 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 63,701 to 64,156
_1484
Position (kb) 62
63 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 61.064 kb on + strand, within Ac3H11_1481 at 61.064 kb on + strand, within Ac3H11_1481 at 61.065 kb on - strand, within Ac3H11_1481 at 61.065 kb on - strand, within Ac3H11_1481 at 61.435 kb on + strand, within Ac3H11_1481 at 61.435 kb on + strand, within Ac3H11_1481 at 61.435 kb on + strand, within Ac3H11_1481 at 61.435 kb on + strand, within Ac3H11_1481 at 61.435 kb on + strand, within Ac3H11_1481 at 61.435 kb on + strand, within Ac3H11_1481 at 61.435 kb on + strand, within Ac3H11_1481 at 61.436 kb on - strand, within Ac3H11_1481 at 61.436 kb on - strand, within Ac3H11_1481 at 61.436 kb on - strand, within Ac3H11_1481 at 61.436 kb on - strand, within Ac3H11_1481 at 61.573 kb on + strand, within Ac3H11_1481 at 61.574 kb on - strand, within Ac3H11_1481 at 61.574 kb on - strand, within Ac3H11_1481 at 61.589 kb on - strand, within Ac3H11_1481 at 61.666 kb on + strand, within Ac3H11_1481 at 61.666 kb on + strand, within Ac3H11_1481 at 61.666 kb on + strand, within Ac3H11_1481 at 62.028 kb on + strand at 62.028 kb on + strand at 62.028 kb on + strand at 62.029 kb on - strand at 62.029 kb on - strand at 62.029 kb on - strand at 62.029 kb on - strand at 62.029 kb on - strand at 62.029 kb on - strand at 62.029 kb on - strand at 62.029 kb on - strand at 62.050 kb on + strand at 62.130 kb on - strand, within Ac3H11_1482 at 62.194 kb on + strand, within Ac3H11_1482 at 62.194 kb on + strand, within Ac3H11_1482 at 62.459 kb on - strand, within Ac3H11_1482 at 62.459 kb on - strand, within Ac3H11_1482 at 62.530 kb on + strand, within Ac3H11_1482 at 62.531 kb on - strand, within Ac3H11_1482 at 62.531 kb on - strand, within Ac3H11_1482 at 62.614 kb on + strand, within Ac3H11_1482 at 62.614 kb on + strand, within Ac3H11_1482 at 62.614 kb on + strand, within Ac3H11_1482 at 62.918 kb on + strand at 63.287 kb on + strand, within Ac3H11_1483 at 63.287 kb on + strand, within Ac3H11_1483 at 63.288 kb on - strand, within Ac3H11_1483 at 63.288 kb on - strand, within Ac3H11_1483 at 63.402 kb on + strand, within Ac3H11_1483 at 63.402 kb on + strand, within Ac3H11_1483 at 63.402 kb on + strand, within Ac3H11_1483 at 63.403 kb on - strand, within Ac3H11_1483 at 63.403 kb on - strand, within Ac3H11_1483 at 63.446 kb on + strand, within Ac3H11_1483 at 63.447 kb on - strand, within Ac3H11_1483 at 63.630 kb on + strand at 63.630 kb on + strand at 63.630 kb on + strand at 63.630 kb on + strand at 63.630 kb on + strand at 63.630 kb on + strand at 63.630 kb on + strand at 63.631 kb on - strand at 63.631 kb on - strand at 63.631 kb on - strand at 63.631 kb on - strand at 63.631 kb on - strand at 63.631 kb on - strand at 63.631 kb on - strand at 63.631 kb on - strand at 63.692 kb on + strand at 63.692 kb on + strand at 63.693 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 61,064 + Ac3H11_1481 0.14 +0.5 61,064 + Ac3H11_1481 0.14 -1.4 61,065 - Ac3H11_1481 0.14 -1.5 61,065 - Ac3H11_1481 0.14 +1.2 61,435 + Ac3H11_1481 0.52 -0.4 61,435 + Ac3H11_1481 0.52 -0.0 61,435 + Ac3H11_1481 0.52 -1.3 61,435 + Ac3H11_1481 0.52 -3.1 61,435 + Ac3H11_1481 0.52 -1.0 61,435 + Ac3H11_1481 0.52 -0.3 61,435 + Ac3H11_1481 0.52 +1.7 61,436 - Ac3H11_1481 0.52 -2.8 61,436 - Ac3H11_1481 0.52 -0.0 61,436 - Ac3H11_1481 0.52 +1.5 61,436 - Ac3H11_1481 0.52 +0.6 61,573 + Ac3H11_1481 0.66 -0.8 61,574 - Ac3H11_1481 0.66 +0.2 61,574 - Ac3H11_1481 0.66 +0.4 61,589 - Ac3H11_1481 0.67 -0.2 61,666 + Ac3H11_1481 0.75 -0.5 61,666 + Ac3H11_1481 0.75 +0.7 61,666 + Ac3H11_1481 0.75 -0.8 62,028 + -0.7 62,028 + -0.8 62,028 + +0.8 62,029 - +1.8 62,029 - +0.2 62,029 - -0.1 62,029 - +2.4 62,029 - +0.7 62,029 - +2.1 62,029 - +0.5 62,029 - +0.5 62,050 + -1.4 62,130 - Ac3H11_1482 0.10 +0.4 62,194 + Ac3H11_1482 0.18 -0.7 62,194 + Ac3H11_1482 0.18 -1.8 62,459 - Ac3H11_1482 0.50 -0.1 62,459 - Ac3H11_1482 0.50 -0.7 62,530 + Ac3H11_1482 0.59 -3.3 62,531 - Ac3H11_1482 0.59 -2.8 62,531 - Ac3H11_1482 0.59 -1.1 62,614 + Ac3H11_1482 0.69 +0.3 62,614 + Ac3H11_1482 0.69 -1.1 62,614 + Ac3H11_1482 0.69 +0.1 62,918 + -1.8 63,287 + Ac3H11_1483 0.54 +0.4 63,287 + Ac3H11_1483 0.54 +0.7 63,288 - Ac3H11_1483 0.54 +0.3 63,288 - Ac3H11_1483 0.54 +0.3 63,402 + Ac3H11_1483 0.69 -0.1 63,402 + Ac3H11_1483 0.69 +0.2 63,402 + Ac3H11_1483 0.69 -0.4 63,403 - Ac3H11_1483 0.70 -2.7 63,403 - Ac3H11_1483 0.70 +0.3 63,446 + Ac3H11_1483 0.75 -0.7 63,447 - Ac3H11_1483 0.75 +0.6 63,630 + -2.8 63,630 + +0.6 63,630 + -0.1 63,630 + +0.5 63,630 + -1.4 63,630 + +0.2 63,630 + +1.4 63,631 - -0.1 63,631 - +1.2 63,631 - -1.0 63,631 - -0.4 63,631 - +0.4 63,631 - +0.5 63,631 - -0.0 63,631 - -0.1 63,692 + -0.8 63,692 + +0.2 63,693 - -1.5
Or see this region's nucleotide sequence