Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1294 and Ac3H11_1295 are separated by 108 nucleotides Ac3H11_1295 and Ac3H11_1296 are separated by 20 nucleotides Ac3H11_1296 and Ac3H11_1297 are separated by 281 nucleotides
Ac3H11_1294 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 109,833 to 110,744
_1294
Ac3H11_1295 - hypothetical protein, at 110,853 to 111,386
_1295
Ac3H11_1296 - FIG00794195: hypothetical protein, at 111,407 to 112,180
_1296
Ac3H11_1297 - Transcriptional regulator, DeoR family, at 112,462 to 113,487
_1297
Position (kb)
111
112
113 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 110.422 kb on - strand, within Ac3H11_1294 at 110.484 kb on + strand, within Ac3H11_1294 at 110.746 kb on + strand at 110.769 kb on + strand at 110.770 kb on - strand at 111.103 kb on + strand, within Ac3H11_1295 at 111.103 kb on + strand, within Ac3H11_1295 at 111.104 kb on - strand, within Ac3H11_1295 at 111.104 kb on - strand, within Ac3H11_1295 at 111.392 kb on + strand at 111.393 kb on - strand at 111.630 kb on + strand, within Ac3H11_1296 at 111.631 kb on - strand, within Ac3H11_1296 at 111.906 kb on + strand, within Ac3H11_1296 at 111.906 kb on + strand, within Ac3H11_1296 at 111.907 kb on - strand, within Ac3H11_1296 at 112.092 kb on + strand, within Ac3H11_1296 at 112.093 kb on - strand, within Ac3H11_1296 at 112.093 kb on - strand, within Ac3H11_1296 at 112.093 kb on - strand, within Ac3H11_1296 at 112.242 kb on - strand at 112.242 kb on - strand at 112.253 kb on + strand at 112.253 kb on + strand at 112.254 kb on - strand at 112.262 kb on + strand at 112.263 kb on - strand at 112.263 kb on - strand at 112.348 kb on + strand at 112.357 kb on + strand at 112.358 kb on - strand at 112.367 kb on - strand at 112.367 kb on - strand at 112.376 kb on - strand at 112.376 kb on - strand at 112.609 kb on + strand, within Ac3H11_1297 at 112.609 kb on + strand, within Ac3H11_1297 at 112.610 kb on - strand, within Ac3H11_1297 at 112.610 kb on - strand, within Ac3H11_1297 at 112.610 kb on - strand, within Ac3H11_1297 at 112.610 kb on - strand, within Ac3H11_1297 at 112.934 kb on - strand, within Ac3H11_1297 at 113.005 kb on + strand, within Ac3H11_1297 at 113.005 kb on + strand, within Ac3H11_1297 at 113.005 kb on + strand, within Ac3H11_1297 at 113.092 kb on + strand, within Ac3H11_1297 at 113.092 kb on + strand, within Ac3H11_1297 at 113.093 kb on - strand, within Ac3H11_1297 at 113.093 kb on - strand, within Ac3H11_1297
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 110,422 - Ac3H11_1294 0.65 -2.2 110,484 + Ac3H11_1294 0.71 +0.7 110,746 + -2.5 110,769 + +0.8 110,770 - +3.2 111,103 + Ac3H11_1295 0.47 -2.9 111,103 + Ac3H11_1295 0.47 +0.6 111,104 - Ac3H11_1295 0.47 -1.8 111,104 - Ac3H11_1295 0.47 -0.4 111,392 + -0.1 111,393 - -0.9 111,630 + Ac3H11_1296 0.29 -0.5 111,631 - Ac3H11_1296 0.29 -1.5 111,906 + Ac3H11_1296 0.64 +1.4 111,906 + Ac3H11_1296 0.64 -0.2 111,907 - Ac3H11_1296 0.65 +1.4 112,092 + Ac3H11_1296 0.89 +0.8 112,093 - Ac3H11_1296 0.89 +1.7 112,093 - Ac3H11_1296 0.89 +0.1 112,093 - Ac3H11_1296 0.89 +0.0 112,242 - +1.2 112,242 - +0.6 112,253 + -1.2 112,253 + -0.4 112,254 - +1.2 112,262 + -3.6 112,263 - +0.7 112,263 - +1.1 112,348 + +0.1 112,357 + -0.4 112,358 - +0.3 112,367 - -1.9 112,367 - -1.9 112,376 - +0.9 112,376 - +0.8 112,609 + Ac3H11_1297 0.14 +0.6 112,609 + Ac3H11_1297 0.14 +1.1 112,610 - Ac3H11_1297 0.14 +1.1 112,610 - Ac3H11_1297 0.14 -2.2 112,610 - Ac3H11_1297 0.14 +0.4 112,610 - Ac3H11_1297 0.14 +1.3 112,934 - Ac3H11_1297 0.46 -0.0 113,005 + Ac3H11_1297 0.53 +0.7 113,005 + Ac3H11_1297 0.53 +1.3 113,005 + Ac3H11_1297 0.53 -0.0 113,092 + Ac3H11_1297 0.61 -0.4 113,092 + Ac3H11_1297 0.61 -0.0 113,093 - Ac3H11_1297 0.62 +0.2 113,093 - Ac3H11_1297 0.62 +0.9
Or see this region's nucleotide sequence