Experiment: D-Cycloserine 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaK1 and dnaJ1 are separated by 141 nucleotides dnaJ1 and Synpcc7942_2075 overlap by 4 nucleotides Synpcc7942_2075 and rsgA overlap by 4 nucleotides
Synpcc7942_2073: dnaK1 - Heat shock protein Hsp70, at 2,150,530 to 2,152,497
dnaK1
Synpcc7942_2074: dnaJ1 - Heat shock protein DnaJ, at 2,152,639 to 2,153,769
dnaJ1
Synpcc7942_2075: Synpcc7942_2075 - hypothetical protein, at 2,153,766 to 2,154,017
_2075
Synpcc7942_2076: rsgA - ribosome-associated GTPase, at 2,154,014 to 2,155,066
rsgA
Position (kb)
2152
2153
2154 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 2151.712 kb on - strand, within dnaK1 at 2151.781 kb on - strand, within dnaK1 at 2151.781 kb on - strand, within dnaK1 at 2151.798 kb on + strand, within dnaK1 at 2151.809 kb on - strand, within dnaK1 at 2152.007 kb on + strand, within dnaK1 at 2152.017 kb on + strand, within dnaK1 at 2152.029 kb on + strand, within dnaK1 at 2152.050 kb on - strand, within dnaK1 at 2152.050 kb on - strand, within dnaK1 at 2152.087 kb on - strand, within dnaK1 at 2152.202 kb on - strand, within dnaK1 at 2152.202 kb on - strand, within dnaK1 at 2152.323 kb on - strand at 2152.323 kb on - strand at 2152.329 kb on - strand at 2152.366 kb on - strand at 2152.383 kb on + strand at 2152.389 kb on - strand at 2152.396 kb on - strand at 2152.413 kb on + strand at 2152.487 kb on - strand at 2152.492 kb on + strand at 2152.495 kb on - strand at 2152.506 kb on + strand at 2152.540 kb on + strand at 2152.542 kb on + strand at 2152.548 kb on - strand at 2152.550 kb on - strand at 2152.566 kb on + strand at 2152.566 kb on + strand at 2152.574 kb on - strand at 2152.579 kb on - strand at 2152.585 kb on + strand at 2152.625 kb on + strand at 2152.625 kb on + strand at 2152.633 kb on - strand at 2152.635 kb on + strand at 2152.635 kb on + strand at 2152.638 kb on - strand at 2152.643 kb on - strand at 2153.149 kb on - strand, within dnaJ1 at 2153.449 kb on - strand, within dnaJ1 at 2153.451 kb on + strand, within dnaJ1 at 2153.484 kb on - strand, within dnaJ1 at 2153.522 kb on + strand, within dnaJ1 at 2153.580 kb on + strand, within dnaJ1 at 2153.580 kb on + strand, within dnaJ1 at 2153.580 kb on + strand, within dnaJ1 at 2153.729 kb on - strand at 2154.008 kb on - strand at 2154.031 kb on - strand at 2154.058 kb on + strand at 2154.058 kb on + strand at 2154.058 kb on + strand at 2154.066 kb on - strand at 2154.079 kb on - strand at 2154.150 kb on + strand, within rsgA at 2154.151 kb on - strand, within rsgA at 2154.192 kb on + strand, within rsgA at 2154.250 kb on - strand, within rsgA at 2154.315 kb on + strand, within rsgA at 2154.354 kb on + strand, within rsgA at 2154.360 kb on + strand, within rsgA at 2154.360 kb on + strand, within rsgA at 2154.360 kb on + strand, within rsgA at 2154.360 kb on + strand, within rsgA at 2154.365 kb on + strand, within rsgA at 2154.365 kb on + strand, within rsgA at 2154.368 kb on - strand, within rsgA at 2154.455 kb on - strand, within rsgA at 2154.464 kb on + strand, within rsgA at 2154.472 kb on - strand, within rsgA at 2154.582 kb on + strand, within rsgA at 2154.604 kb on + strand, within rsgA at 2154.615 kb on + strand, within rsgA at 2154.616 kb on + strand, within rsgA at 2154.664 kb on + strand, within rsgA at 2154.673 kb on - strand, within rsgA at 2154.715 kb on + strand, within rsgA at 2154.718 kb on - strand, within rsgA at 2154.723 kb on - strand, within rsgA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.03125 mg/ml remove 2,151,712 - dnaK1 Synpcc7942_2073 0.60 +0.4 2,151,781 - dnaK1 Synpcc7942_2073 0.64 +1.1 2,151,781 - dnaK1 Synpcc7942_2073 0.64 +0.8 2,151,798 + dnaK1 Synpcc7942_2073 0.64 +2.0 2,151,809 - dnaK1 Synpcc7942_2073 0.65 -0.1 2,152,007 + dnaK1 Synpcc7942_2073 0.75 +0.5 2,152,017 + dnaK1 Synpcc7942_2073 0.76 +0.2 2,152,029 + dnaK1 Synpcc7942_2073 0.76 -1.1 2,152,050 - dnaK1 Synpcc7942_2073 0.77 -2.1 2,152,050 - dnaK1 Synpcc7942_2073 0.77 -0.1 2,152,087 - dnaK1 Synpcc7942_2073 0.79 +0.2 2,152,202 - dnaK1 Synpcc7942_2073 0.85 +1.6 2,152,202 - dnaK1 Synpcc7942_2073 0.85 +0.3 2,152,323 - +1.5 2,152,323 - +0.7 2,152,329 - -0.7 2,152,366 - +0.6 2,152,383 + +2.7 2,152,389 - +1.5 2,152,396 - -0.1 2,152,413 + +0.0 2,152,487 - +2.5 2,152,492 + -0.5 2,152,495 - +1.6 2,152,506 + -0.3 2,152,540 + +0.3 2,152,542 + +0.1 2,152,548 - -0.1 2,152,550 - +1.1 2,152,566 + +1.1 2,152,566 + +0.9 2,152,574 - +0.3 2,152,579 - +0.8 2,152,585 + +1.3 2,152,625 + -1.1 2,152,625 + +0.6 2,152,633 - -1.7 2,152,635 + +0.9 2,152,635 + -0.0 2,152,638 - +0.7 2,152,643 - -1.1 2,153,149 - dnaJ1 Synpcc7942_2074 0.45 -2.1 2,153,449 - dnaJ1 Synpcc7942_2074 0.72 -0.1 2,153,451 + dnaJ1 Synpcc7942_2074 0.72 -1.2 2,153,484 - dnaJ1 Synpcc7942_2074 0.75 -0.4 2,153,522 + dnaJ1 Synpcc7942_2074 0.78 -2.9 2,153,580 + dnaJ1 Synpcc7942_2074 0.83 +2.9 2,153,580 + dnaJ1 Synpcc7942_2074 0.83 -3.3 2,153,580 + dnaJ1 Synpcc7942_2074 0.83 -2.4 2,153,729 - -0.1 2,154,008 - -1.7 2,154,031 - +0.6 2,154,058 + -2.7 2,154,058 + -4.2 2,154,058 + -1.7 2,154,066 - -5.2 2,154,079 - -2.5 2,154,150 + rsgA Synpcc7942_2076 0.13 -3.2 2,154,151 - rsgA Synpcc7942_2076 0.13 -3.7 2,154,192 + rsgA Synpcc7942_2076 0.17 -3.7 2,154,250 - rsgA Synpcc7942_2076 0.22 -5.5 2,154,315 + rsgA Synpcc7942_2076 0.29 -1.1 2,154,354 + rsgA Synpcc7942_2076 0.32 -3.2 2,154,360 + rsgA Synpcc7942_2076 0.33 -4.4 2,154,360 + rsgA Synpcc7942_2076 0.33 -3.4 2,154,360 + rsgA Synpcc7942_2076 0.33 -2.7 2,154,360 + rsgA Synpcc7942_2076 0.33 -4.2 2,154,365 + rsgA Synpcc7942_2076 0.33 -4.7 2,154,365 + rsgA Synpcc7942_2076 0.33 -2.9 2,154,368 - rsgA Synpcc7942_2076 0.34 -3.8 2,154,455 - rsgA Synpcc7942_2076 0.42 -3.7 2,154,464 + rsgA Synpcc7942_2076 0.43 -4.5 2,154,472 - rsgA Synpcc7942_2076 0.43 -3.5 2,154,582 + rsgA Synpcc7942_2076 0.54 -5.0 2,154,604 + rsgA Synpcc7942_2076 0.56 -3.2 2,154,615 + rsgA Synpcc7942_2076 0.57 -6.2 2,154,616 + rsgA Synpcc7942_2076 0.57 -5.1 2,154,664 + rsgA Synpcc7942_2076 0.62 -2.1 2,154,673 - rsgA Synpcc7942_2076 0.63 -2.4 2,154,715 + rsgA Synpcc7942_2076 0.67 -3.0 2,154,718 - rsgA Synpcc7942_2076 0.67 -4.0 2,154,723 - rsgA Synpcc7942_2076 0.67 -3.5
Or see this region's nucleotide sequence