Experiment: D-Cycloserine 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1330 and Synpcc7942_1331 are separated by 125 nucleotides Synpcc7942_1331 and Synpcc7942_1332 are separated by 4 nucleotides Synpcc7942_1332 and Synpcc7942_1333 overlap by 1 nucleotides Synpcc7942_1333 and trpE overlap by 4 nucleotides
Synpcc7942_1330: Synpcc7942_1330 - hypothetical protein, at 1,362,508 to 1,363,164
_1330
Synpcc7942_1331: Synpcc7942_1331 - CAB/ELIP/HLIP superfamily protein, at 1,363,290 to 1,363,466
_1331
Synpcc7942_1332: Synpcc7942_1332 - hypothetical protein, at 1,363,471 to 1,364,091
_1332
Synpcc7942_1333: Synpcc7942_1333 - ExsB, at 1,364,091 to 1,364,786
_1333
Synpcc7942_1334: trpE - aminodeoxychorismate synthase, subunit I, at 1,364,783 to 1,366,180
trpE
Position (kb)
1364
1365 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1363.113 kb on + strand at 1363.134 kb on + strand at 1363.142 kb on - strand at 1363.149 kb on + strand at 1363.157 kb on - strand at 1363.157 kb on - strand at 1363.157 kb on - strand at 1363.157 kb on - strand at 1363.166 kb on - strand at 1363.167 kb on - strand at 1363.212 kb on - strand at 1363.250 kb on + strand at 1363.354 kb on + strand, within Synpcc7942_1331 at 1363.362 kb on - strand, within Synpcc7942_1331 at 1363.402 kb on + strand, within Synpcc7942_1331 at 1363.467 kb on + strand at 1363.469 kb on - strand at 1363.475 kb on - strand at 1363.485 kb on - strand at 1363.485 kb on - strand at 1363.490 kb on - strand at 1363.531 kb on + strand at 1363.539 kb on - strand, within Synpcc7942_1332 at 1363.552 kb on - strand, within Synpcc7942_1332 at 1363.557 kb on + strand, within Synpcc7942_1332 at 1363.589 kb on + strand, within Synpcc7942_1332 at 1363.597 kb on - strand, within Synpcc7942_1332 at 1363.616 kb on - strand, within Synpcc7942_1332 at 1363.631 kb on - strand, within Synpcc7942_1332 at 1363.668 kb on + strand, within Synpcc7942_1332 at 1363.681 kb on + strand, within Synpcc7942_1332 at 1363.692 kb on + strand, within Synpcc7942_1332 at 1363.692 kb on + strand, within Synpcc7942_1332 at 1363.700 kb on - strand, within Synpcc7942_1332 at 1363.749 kb on + strand, within Synpcc7942_1332 at 1363.770 kb on + strand, within Synpcc7942_1332 at 1363.770 kb on + strand, within Synpcc7942_1332 at 1363.771 kb on + strand, within Synpcc7942_1332 at 1363.779 kb on - strand, within Synpcc7942_1332 at 1363.802 kb on - strand, within Synpcc7942_1332 at 1363.829 kb on + strand, within Synpcc7942_1332 at 1363.873 kb on + strand, within Synpcc7942_1332 at 1363.881 kb on - strand, within Synpcc7942_1332 at 1363.938 kb on + strand, within Synpcc7942_1332 at 1363.942 kb on + strand, within Synpcc7942_1332 at 1363.985 kb on - strand, within Synpcc7942_1332 at 1363.985 kb on - strand, within Synpcc7942_1332 at 1363.989 kb on + strand, within Synpcc7942_1332 at 1364.108 kb on - strand at 1364.131 kb on - strand at 1364.145 kb on + strand at 1364.172 kb on - strand, within Synpcc7942_1333 at 1364.189 kb on + strand, within Synpcc7942_1333 at 1364.204 kb on - strand, within Synpcc7942_1333 at 1364.259 kb on - strand, within Synpcc7942_1333 at 1364.307 kb on - strand, within Synpcc7942_1333 at 1364.312 kb on - strand, within Synpcc7942_1333 at 1364.465 kb on + strand, within Synpcc7942_1333 at 1364.553 kb on + strand, within Synpcc7942_1333 at 1364.561 kb on - strand, within Synpcc7942_1333 at 1364.630 kb on - strand, within Synpcc7942_1333 at 1364.676 kb on - strand, within Synpcc7942_1333 at 1365.024 kb on + strand, within trpE at 1365.121 kb on + strand, within trpE at 1365.489 kb on + strand, within trpE
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.03125 mg/ml remove 1,363,113 + -0.5 1,363,134 + +0.2 1,363,142 - +0.3 1,363,149 + -0.6 1,363,157 - -1.2 1,363,157 - +1.2 1,363,157 - +1.4 1,363,157 - +0.8 1,363,166 - -2.1 1,363,167 - -0.1 1,363,212 - +2.1 1,363,250 + -0.1 1,363,354 + Synpcc7942_1331 0.36 -3.1 1,363,362 - Synpcc7942_1331 0.41 -1.7 1,363,402 + Synpcc7942_1331 0.63 -1.1 1,363,467 + -0.5 1,363,469 - +1.7 1,363,475 - -0.8 1,363,485 - -2.9 1,363,485 - -1.3 1,363,490 - +1.2 1,363,531 + -2.1 1,363,539 - Synpcc7942_1332 0.11 -3.7 1,363,552 - Synpcc7942_1332 0.13 -2.9 1,363,557 + Synpcc7942_1332 0.14 -3.4 1,363,589 + Synpcc7942_1332 0.19 -4.3 1,363,597 - Synpcc7942_1332 0.20 -4.5 1,363,616 - Synpcc7942_1332 0.23 -4.1 1,363,631 - Synpcc7942_1332 0.26 -2.1 1,363,668 + Synpcc7942_1332 0.32 -2.9 1,363,681 + Synpcc7942_1332 0.34 -4.2 1,363,692 + Synpcc7942_1332 0.36 -3.6 1,363,692 + Synpcc7942_1332 0.36 -2.1 1,363,700 - Synpcc7942_1332 0.37 -3.1 1,363,749 + Synpcc7942_1332 0.45 -3.6 1,363,770 + Synpcc7942_1332 0.48 -3.8 1,363,770 + Synpcc7942_1332 0.48 -3.3 1,363,771 + Synpcc7942_1332 0.48 -2.9 1,363,779 - Synpcc7942_1332 0.50 -2.9 1,363,802 - Synpcc7942_1332 0.53 -2.7 1,363,829 + Synpcc7942_1332 0.58 -1.7 1,363,873 + Synpcc7942_1332 0.65 -3.8 1,363,881 - Synpcc7942_1332 0.66 -3.3 1,363,938 + Synpcc7942_1332 0.75 -2.7 1,363,942 + Synpcc7942_1332 0.76 -2.1 1,363,985 - Synpcc7942_1332 0.83 -3.1 1,363,985 - Synpcc7942_1332 0.83 -4.1 1,363,989 + Synpcc7942_1332 0.83 -3.3 1,364,108 - +0.1 1,364,131 - -4.0 1,364,145 + -3.8 1,364,172 - Synpcc7942_1333 0.12 +2.5 1,364,189 + Synpcc7942_1333 0.14 -3.1 1,364,204 - Synpcc7942_1333 0.16 -2.4 1,364,259 - Synpcc7942_1333 0.24 -4.7 1,364,307 - Synpcc7942_1333 0.31 -3.7 1,364,312 - Synpcc7942_1333 0.32 -2.3 1,364,465 + Synpcc7942_1333 0.54 -3.7 1,364,553 + Synpcc7942_1333 0.66 -2.4 1,364,561 - Synpcc7942_1333 0.68 -4.2 1,364,630 - Synpcc7942_1333 0.77 -2.7 1,364,676 - Synpcc7942_1333 0.84 -3.3 1,365,024 + trpE Synpcc7942_1334 0.17 -2.2 1,365,121 + trpE Synpcc7942_1334 0.24 -2.7 1,365,489 + trpE Synpcc7942_1334 0.51 -0.7
Or see this region's nucleotide sequence