Experiment: D-Cycloserine 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt coaE and Synpcc7942_0274 are separated by 37 nucleotides Synpcc7942_0274 and Synpcc7942_0275 overlap by 11 nucleotides Synpcc7942_0275 and glcD are separated by 11 nucleotides glcD and ligA are separated by 19 nucleotides
Synpcc7942_0273: coaE - Dephospho-CoA kinase, at 268,662 to 269,291
coaE
Synpcc7942_0274: Synpcc7942_0274 - hypothetical protein, at 269,329 to 269,667
_0274
Synpcc7942_0275: Synpcc7942_0275 - hypothetical protein, at 269,657 to 269,989
_0275
Synpcc7942_0276: glcD - glycolate oxidase subunit GlcD, at 270,001 to 271,473
glcD
Synpcc7942_0277: ligA - NAD-dependent DNA ligase LigA, at 271,493 to 273,532
ligA
Position (kb) 270
271
272 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 269.351 kb on - strand at 269.804 kb on + strand, within Synpcc7942_0275 at 270.162 kb on + strand, within glcD at 270.219 kb on + strand, within glcD at 270.273 kb on - strand, within glcD at 270.395 kb on - strand, within glcD at 270.411 kb on + strand, within glcD at 270.419 kb on - strand, within glcD at 270.431 kb on + strand, within glcD at 270.497 kb on - strand, within glcD at 270.598 kb on + strand, within glcD at 270.598 kb on + strand, within glcD at 270.701 kb on - strand, within glcD at 270.701 kb on - strand, within glcD at 270.730 kb on + strand, within glcD at 270.738 kb on - strand, within glcD at 270.753 kb on + strand, within glcD at 270.854 kb on - strand, within glcD at 270.881 kb on - strand, within glcD at 270.881 kb on - strand, within glcD at 270.881 kb on - strand, within glcD at 270.881 kb on - strand, within glcD at 270.881 kb on - strand, within glcD at 270.891 kb on - strand, within glcD at 270.897 kb on - strand, within glcD at 270.931 kb on - strand, within glcD at 270.941 kb on - strand, within glcD at 270.953 kb on - strand, within glcD at 270.982 kb on + strand, within glcD at 270.988 kb on - strand, within glcD at 271.032 kb on + strand, within glcD at 271.218 kb on + strand, within glcD at 271.342 kb on - strand at 271.344 kb on + strand at 271.352 kb on - strand at 271.352 kb on - strand at 271.399 kb on + strand at 271.399 kb on + strand at 271.407 kb on - strand at 271.407 kb on - strand at 271.457 kb on + strand at 271.474 kb on + strand at 271.493 kb on + strand at 271.498 kb on + strand at 271.509 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.03125 mg/ml remove 269,351 - -2.3 269,804 + Synpcc7942_0275 0.44 +0.5 270,162 + glcD Synpcc7942_0276 0.11 +0.2 270,219 + glcD Synpcc7942_0276 0.15 -0.9 270,273 - glcD Synpcc7942_0276 0.18 -1.2 270,395 - glcD Synpcc7942_0276 0.27 +1.1 270,411 + glcD Synpcc7942_0276 0.28 -0.7 270,419 - glcD Synpcc7942_0276 0.28 +0.5 270,431 + glcD Synpcc7942_0276 0.29 +0.3 270,497 - glcD Synpcc7942_0276 0.34 -0.9 270,598 + glcD Synpcc7942_0276 0.41 -3.7 270,598 + glcD Synpcc7942_0276 0.41 -0.7 270,701 - glcD Synpcc7942_0276 0.48 -2.9 270,701 - glcD Synpcc7942_0276 0.48 +1.6 270,730 + glcD Synpcc7942_0276 0.49 -3.1 270,738 - glcD Synpcc7942_0276 0.50 +0.6 270,753 + glcD Synpcc7942_0276 0.51 -1.2 270,854 - glcD Synpcc7942_0276 0.58 +1.9 270,881 - glcD Synpcc7942_0276 0.60 -1.7 270,881 - glcD Synpcc7942_0276 0.60 -1.4 270,881 - glcD Synpcc7942_0276 0.60 +0.9 270,881 - glcD Synpcc7942_0276 0.60 +0.6 270,881 - glcD Synpcc7942_0276 0.60 -0.5 270,891 - glcD Synpcc7942_0276 0.60 -1.0 270,897 - glcD Synpcc7942_0276 0.61 -1.1 270,931 - glcD Synpcc7942_0276 0.63 -1.2 270,941 - glcD Synpcc7942_0276 0.64 +0.7 270,953 - glcD Synpcc7942_0276 0.65 -2.7 270,982 + glcD Synpcc7942_0276 0.67 -1.1 270,988 - glcD Synpcc7942_0276 0.67 +1.7 271,032 + glcD Synpcc7942_0276 0.70 -1.1 271,218 + glcD Synpcc7942_0276 0.83 +0.3 271,342 - +0.2 271,344 + -1.1 271,352 - -1.4 271,352 - +2.2 271,399 + -1.3 271,399 + -0.5 271,407 - -1.4 271,407 - -2.1 271,457 + +0.7 271,474 + +0.5 271,493 + +0.5 271,498 + +0.1 271,509 - +0.9
Or see this region's nucleotide sequence