Experiment: D-Cycloserine 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0191 and ycf60 are separated by 89 nucleotides ycf60 and Synpcc7942_0193 are separated by 60 nucleotides Synpcc7942_0193 and polA are separated by 183 nucleotides
Synpcc7942_0191: Synpcc7942_0191 - Serine--glyoxylate transaminase, at 190,472 to 191,620
_0191
Synpcc7942_0192: ycf60 - conserved hypothetical protein YCF60, at 191,710 to 192,186
ycf60
Synpcc7942_0193: Synpcc7942_0193 - hypothetical protein, at 192,247 to 192,471
_0193
Synpcc7942_0194: polA - DNA polymerase I, at 192,655 to 195,516
polA
Position (kb)
192
193 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 191.661 kb on + strand at 191.710 kb on + strand at 191.806 kb on - strand, within ycf60 at 191.818 kb on - strand, within ycf60 at 191.832 kb on - strand, within ycf60 at 191.859 kb on + strand, within ycf60 at 191.866 kb on + strand, within ycf60 at 191.866 kb on + strand, within ycf60 at 191.867 kb on - strand, within ycf60 at 191.874 kb on - strand, within ycf60 at 191.941 kb on - strand, within ycf60 at 192.062 kb on - strand, within ycf60 at 192.097 kb on + strand, within ycf60 at 192.098 kb on - strand, within ycf60 at 192.165 kb on + strand at 192.165 kb on + strand at 192.173 kb on - strand at 192.174 kb on - strand at 192.190 kb on - strand at 192.190 kb on - strand at 192.191 kb on + strand at 192.191 kb on + strand at 192.199 kb on - strand at 192.211 kb on - strand at 192.220 kb on + strand at 192.221 kb on - strand at 192.231 kb on + strand at 192.249 kb on + strand at 192.328 kb on + strand, within Synpcc7942_0193 at 192.328 kb on + strand, within Synpcc7942_0193 at 192.335 kb on - strand, within Synpcc7942_0193 at 192.336 kb on - strand, within Synpcc7942_0193 at 192.369 kb on + strand, within Synpcc7942_0193 at 192.377 kb on - strand, within Synpcc7942_0193 at 192.478 kb on + strand at 192.548 kb on + strand at 192.548 kb on + strand at 192.548 kb on + strand at 192.556 kb on - strand at 192.570 kb on - strand at 192.571 kb on - strand at 192.582 kb on + strand at 192.583 kb on - strand at 192.583 kb on - strand at 192.597 kb on + strand at 192.605 kb on - strand at 192.673 kb on + strand at 192.673 kb on + strand at 192.681 kb on - strand at 192.698 kb on - strand at 192.700 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.03125 mg/ml remove 191,661 + -0.1 191,710 + -1.7 191,806 - ycf60 Synpcc7942_0192 0.20 -1.4 191,818 - ycf60 Synpcc7942_0192 0.23 -0.7 191,832 - ycf60 Synpcc7942_0192 0.26 +0.7 191,859 + ycf60 Synpcc7942_0192 0.31 -1.0 191,866 + ycf60 Synpcc7942_0192 0.33 +0.6 191,866 + ycf60 Synpcc7942_0192 0.33 +0.4 191,867 - ycf60 Synpcc7942_0192 0.33 -0.1 191,874 - ycf60 Synpcc7942_0192 0.34 +0.6 191,941 - ycf60 Synpcc7942_0192 0.48 -0.5 192,062 - ycf60 Synpcc7942_0192 0.74 +0.2 192,097 + ycf60 Synpcc7942_0192 0.81 -1.7 192,098 - ycf60 Synpcc7942_0192 0.81 +1.2 192,165 + +2.7 192,165 + -0.9 192,173 - +1.5 192,174 - +0.8 192,190 - -0.5 192,190 - -0.7 192,191 + -1.6 192,191 + -0.1 192,199 - +0.2 192,211 - -0.4 192,220 + -0.9 192,221 - -2.9 192,231 + -0.7 192,249 + +1.1 192,328 + Synpcc7942_0193 0.36 +0.3 192,328 + Synpcc7942_0193 0.36 -2.1 192,335 - Synpcc7942_0193 0.39 -2.3 192,336 - Synpcc7942_0193 0.40 +1.4 192,369 + Synpcc7942_0193 0.54 -0.9 192,377 - Synpcc7942_0193 0.58 -2.6 192,478 + +0.2 192,548 + +0.6 192,548 + -1.0 192,548 + -3.8 192,556 - +0.2 192,570 - +1.7 192,571 - -0.7 192,582 + -3.6 192,583 - +0.3 192,583 - +0.2 192,597 + +0.1 192,605 - +0.1 192,673 + +1.8 192,673 + +0.5 192,681 - -0.3 192,698 - -0.1 192,700 - -3.6
Or see this region's nucleotide sequence