Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_63 and Pf1N1B4_64 are separated by 109 nucleotides Pf1N1B4_64 and Pf1N1B4_65 are separated by 194 nucleotides Pf1N1B4_65 and Pf1N1B4_66 are separated by 61 nucleotides Pf1N1B4_66 and Pf1N1B4_67 are separated by 152 nucleotides
Pf1N1B4_63 - Phosphonoacetaldehyde phosphonohydrolase-related protein, at 66,737 to 67,357
_63
Pf1N1B4_64 - Chromosome segregation ATPases, at 67,467 to 67,730
_64
Pf1N1B4_65 - NADPH dependent preQ0 reductase (EC 1.7.1.13), at 67,925 to 68,755
_65
Pf1N1B4_66 - Copper tolerance protein, at 68,817 to 69,353
_66
Pf1N1B4_67 - Copper-sensing two-component system response regulator CusR, at 69,506 to 70,186
_67
Position (kb)
67
68
69 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 66.991 kb on + strand at 66.992 kb on - strand at 66.992 kb on - strand, within Pf1N1B4_63 at 67.322 kb on - strand at 67.390 kb on - strand at 67.394 kb on + strand at 67.394 kb on + strand at 67.395 kb on - strand at 67.395 kb on - strand at 67.408 kb on - strand at 67.408 kb on - strand at 67.433 kb on + strand at 67.434 kb on - strand at 67.451 kb on + strand at 67.741 kb on + strand at 67.741 kb on + strand at 67.838 kb on + strand at 67.838 kb on + strand at 67.839 kb on - strand at 67.839 kb on - strand at 67.923 kb on + strand at 67.923 kb on + strand at 67.923 kb on + strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.927 kb on - strand at 67.927 kb on - strand at 68.011 kb on - strand, within Pf1N1B4_65 at 68.011 kb on - strand, within Pf1N1B4_65 at 68.019 kb on + strand, within Pf1N1B4_65 at 68.019 kb on + strand, within Pf1N1B4_65 at 68.019 kb on + strand, within Pf1N1B4_65 at 68.020 kb on - strand, within Pf1N1B4_65 at 68.020 kb on - strand, within Pf1N1B4_65 at 68.020 kb on - strand, within Pf1N1B4_65 at 68.020 kb on - strand, within Pf1N1B4_65 at 68.122 kb on - strand, within Pf1N1B4_65 at 68.122 kb on - strand, within Pf1N1B4_65 at 68.232 kb on - strand, within Pf1N1B4_65 at 68.245 kb on + strand, within Pf1N1B4_65 at 68.246 kb on - strand at 68.292 kb on + strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.484 kb on + strand, within Pf1N1B4_65 at 68.485 kb on - strand, within Pf1N1B4_65 at 68.485 kb on - strand, within Pf1N1B4_65 at 68.485 kb on - strand, within Pf1N1B4_65 at 68.619 kb on + strand, within Pf1N1B4_65 at 68.619 kb on + strand, within Pf1N1B4_65 at 68.619 kb on + strand at 68.620 kb on - strand, within Pf1N1B4_65 at 68.620 kb on - strand, within Pf1N1B4_65 at 68.698 kb on - strand at 68.698 kb on - strand at 68.709 kb on + strand at 68.710 kb on - strand at 68.871 kb on - strand, within Pf1N1B4_66 at 69.038 kb on + strand, within Pf1N1B4_66 at 69.045 kb on - strand, within Pf1N1B4_66 at 69.045 kb on - strand, within Pf1N1B4_66 at 69.045 kb on - strand, within Pf1N1B4_66 at 69.045 kb on - strand, within Pf1N1B4_66 at 69.045 kb on - strand at 69.045 kb on - strand, within Pf1N1B4_66 at 69.129 kb on + strand, within Pf1N1B4_66 at 69.130 kb on - strand, within Pf1N1B4_66 at 69.130 kb on - strand, within Pf1N1B4_66 at 69.141 kb on - strand, within Pf1N1B4_66 at 69.141 kb on - strand, within Pf1N1B4_66 at 69.141 kb on - strand, within Pf1N1B4_66 at 69.141 kb on - strand, within Pf1N1B4_66 at 69.141 kb on - strand at 69.150 kb on + strand, within Pf1N1B4_66 at 69.207 kb on - strand, within Pf1N1B4_66 at 69.207 kb on - strand, within Pf1N1B4_66 at 69.271 kb on + strand, within Pf1N1B4_66 at 69.330 kb on + strand at 69.330 kb on + strand at 69.395 kb on + strand at 69.432 kb on + strand at 69.432 kb on + strand at 69.433 kb on - strand at 69.433 kb on - strand at 69.433 kb on - strand at 69.433 kb on - strand at 69.473 kb on + strand at 69.474 kb on - strand at 69.474 kb on - strand at 69.476 kb on - strand at 69.476 kb on - strand at 69.639 kb on - strand, within Pf1N1B4_67 at 69.729 kb on + strand, within Pf1N1B4_67
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 66,991 + -0.3 66,992 - -0.3 66,992 - Pf1N1B4_63 0.41 +0.6 67,322 - -1.3 67,390 - -0.0 67,394 + +0.5 67,394 + +0.3 67,395 - +1.5 67,395 - -1.9 67,408 - +0.2 67,408 - +0.3 67,433 + +0.3 67,434 - -0.9 67,451 + +0.7 67,741 + +0.9 67,741 + +1.5 67,838 + +0.3 67,838 + +0.2 67,839 - +0.4 67,839 - -0.3 67,923 + +0.2 67,923 + +1.3 67,923 + +2.1 67,924 - +1.7 67,924 - -0.1 67,924 - -0.3 67,924 - +0.7 67,924 - -0.1 67,924 - -1.9 67,924 - +0.1 67,924 - -0.3 67,927 - -0.3 67,927 - -1.1 68,011 - Pf1N1B4_65 0.10 -1.0 68,011 - Pf1N1B4_65 0.10 +0.4 68,019 + Pf1N1B4_65 0.11 -1.9 68,019 + Pf1N1B4_65 0.11 -0.7 68,019 + Pf1N1B4_65 0.11 -1.6 68,020 - Pf1N1B4_65 0.11 +0.1 68,020 - Pf1N1B4_65 0.11 +1.1 68,020 - Pf1N1B4_65 0.11 +0.3 68,020 - Pf1N1B4_65 0.11 -0.3 68,122 - Pf1N1B4_65 0.24 +0.7 68,122 - Pf1N1B4_65 0.24 -0.8 68,232 - Pf1N1B4_65 0.37 -1.1 68,245 + Pf1N1B4_65 0.39 -1.9 68,246 - -1.3 68,292 + Pf1N1B4_65 0.44 +0.7 68,293 - Pf1N1B4_65 0.44 +1.1 68,293 - Pf1N1B4_65 0.44 -0.3 68,293 - Pf1N1B4_65 0.44 -2.3 68,293 - Pf1N1B4_65 0.44 -1.5 68,293 - Pf1N1B4_65 0.44 -0.9 68,293 - Pf1N1B4_65 0.44 +0.1 68,484 + Pf1N1B4_65 0.67 -0.0 68,485 - Pf1N1B4_65 0.67 -0.0 68,485 - Pf1N1B4_65 0.67 -0.9 68,485 - Pf1N1B4_65 0.67 -0.4 68,619 + Pf1N1B4_65 0.84 -0.3 68,619 + Pf1N1B4_65 0.84 -0.3 68,619 + -0.3 68,620 - Pf1N1B4_65 0.84 +1.3 68,620 - Pf1N1B4_65 0.84 -1.3 68,698 - -0.9 68,698 - -1.4 68,709 + +1.1 68,710 - -0.9 68,871 - Pf1N1B4_66 0.10 -0.3 69,038 + Pf1N1B4_66 0.41 -0.3 69,045 - Pf1N1B4_66 0.42 +0.7 69,045 - Pf1N1B4_66 0.42 -1.3 69,045 - Pf1N1B4_66 0.42 +1.3 69,045 - Pf1N1B4_66 0.42 -0.3 69,045 - +2.3 69,045 - Pf1N1B4_66 0.42 +0.9 69,129 + Pf1N1B4_66 0.58 -2.6 69,130 - Pf1N1B4_66 0.58 -0.1 69,130 - Pf1N1B4_66 0.58 +1.7 69,141 - Pf1N1B4_66 0.60 -2.4 69,141 - Pf1N1B4_66 0.60 +0.4 69,141 - Pf1N1B4_66 0.60 +0.7 69,141 - Pf1N1B4_66 0.60 -2.3 69,141 - -0.3 69,150 + Pf1N1B4_66 0.62 -0.0 69,207 - Pf1N1B4_66 0.73 -0.3 69,207 - Pf1N1B4_66 0.73 +1.3 69,271 + Pf1N1B4_66 0.85 +1.7 69,330 + -0.3 69,330 + +1.1 69,395 + -0.3 69,432 + -1.5 69,432 + +0.0 69,433 - +1.5 69,433 - +0.3 69,433 - +0.1 69,433 - +0.5 69,473 + +0.7 69,474 - -1.3 69,474 - -2.9 69,476 - -0.3 69,476 - -1.3 69,639 - Pf1N1B4_67 0.20 -1.7 69,729 + Pf1N1B4_67 0.33 +1.1
Or see this region's nucleotide sequence