Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4934 and Pf1N1B4_4935 overlap by 4 nucleotides Pf1N1B4_4935 and Pf1N1B4_4936 are separated by 128 nucleotides
Pf1N1B4_4934 - DNA damage response nuclease <small>(from data)</small>, at 5,468,400 to 5,470,052
_4934
Pf1N1B4_4935 - DinG family ATP-dependent helicase CPE1197, at 5,470,049 to 5,472,328
_4935
Pf1N1B4_4936 - hypothetical protein, at 5,472,457 to 5,473,362
_4936
Position (kb) 5470
5471
5472
5473 Strain fitness (log2 ratio) -2
-1
0
1 at 5469.349 kb on + strand, within Pf1N1B4_4934 at 5469.519 kb on + strand, within Pf1N1B4_4934 at 5469.519 kb on + strand at 5469.520 kb on - strand, within Pf1N1B4_4934 at 5469.705 kb on + strand, within Pf1N1B4_4934 at 5469.706 kb on - strand, within Pf1N1B4_4934 at 5469.706 kb on - strand, within Pf1N1B4_4934 at 5469.730 kb on - strand at 5469.764 kb on - strand at 5470.055 kb on + strand at 5470.295 kb on + strand, within Pf1N1B4_4935 at 5470.296 kb on - strand at 5470.361 kb on + strand, within Pf1N1B4_4935 at 5470.479 kb on + strand at 5470.479 kb on + strand, within Pf1N1B4_4935 at 5470.929 kb on - strand at 5471.031 kb on - strand at 5471.031 kb on - strand, within Pf1N1B4_4935 at 5471.067 kb on + strand, within Pf1N1B4_4935 at 5471.067 kb on + strand at 5471.079 kb on - strand, within Pf1N1B4_4935 at 5471.079 kb on - strand at 5471.084 kb on + strand, within Pf1N1B4_4935 at 5471.085 kb on - strand, within Pf1N1B4_4935 at 5471.088 kb on - strand, within Pf1N1B4_4935 at 5471.132 kb on - strand, within Pf1N1B4_4935 at 5471.195 kb on + strand, within Pf1N1B4_4935 at 5471.196 kb on - strand at 5471.196 kb on - strand, within Pf1N1B4_4935 at 5471.196 kb on - strand, within Pf1N1B4_4935 at 5471.548 kb on - strand, within Pf1N1B4_4935 at 5471.756 kb on + strand, within Pf1N1B4_4935 at 5471.831 kb on + strand, within Pf1N1B4_4935 at 5471.832 kb on - strand, within Pf1N1B4_4935 at 5471.977 kb on + strand, within Pf1N1B4_4935 at 5471.977 kb on + strand, within Pf1N1B4_4935 at 5472.149 kb on + strand at 5472.150 kb on - strand at 5472.150 kb on - strand at 5472.457 kb on - strand at 5472.475 kb on + strand at 5472.597 kb on + strand at 5472.649 kb on + strand, within Pf1N1B4_4936 at 5472.649 kb on + strand, within Pf1N1B4_4936 at 5472.662 kb on - strand, within Pf1N1B4_4936 at 5473.135 kb on + strand, within Pf1N1B4_4936 at 5473.135 kb on + strand, within Pf1N1B4_4936 at 5473.187 kb on - strand at 5473.187 kb on - strand, within Pf1N1B4_4936 at 5473.188 kb on + strand, within Pf1N1B4_4936 at 5473.188 kb on + strand, within Pf1N1B4_4936 at 5473.188 kb on + strand, within Pf1N1B4_4936 at 5473.189 kb on - strand, within Pf1N1B4_4936 at 5473.225 kb on + strand, within Pf1N1B4_4936 at 5473.226 kb on - strand, within Pf1N1B4_4936 at 5473.226 kb on - strand, within Pf1N1B4_4936 at 5473.292 kb on - strand at 5473.318 kb on + strand at 5473.318 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 5,469,349 + Pf1N1B4_4934 0.57 +1.0 5,469,519 + Pf1N1B4_4934 0.68 +1.1 5,469,519 + +0.4 5,469,520 - Pf1N1B4_4934 0.68 +0.3 5,469,705 + Pf1N1B4_4934 0.79 -0.9 5,469,706 - Pf1N1B4_4934 0.79 -0.3 5,469,706 - Pf1N1B4_4934 0.79 -0.2 5,469,730 - -0.6 5,469,764 - +1.0 5,470,055 + -1.6 5,470,295 + Pf1N1B4_4935 0.11 -0.6 5,470,296 - -0.6 5,470,361 + Pf1N1B4_4935 0.14 +0.1 5,470,479 + +0.4 5,470,479 + Pf1N1B4_4935 0.19 +1.7 5,470,929 - +0.4 5,471,031 - +0.0 5,471,031 - Pf1N1B4_4935 0.43 +0.4 5,471,067 + Pf1N1B4_4935 0.45 -0.6 5,471,067 + +0.0 5,471,079 - Pf1N1B4_4935 0.45 -0.6 5,471,079 - -0.6 5,471,084 + Pf1N1B4_4935 0.45 +1.0 5,471,085 - Pf1N1B4_4935 0.45 -1.7 5,471,088 - Pf1N1B4_4935 0.46 +0.4 5,471,132 - Pf1N1B4_4935 0.47 -0.1 5,471,195 + Pf1N1B4_4935 0.50 +0.3 5,471,196 - +1.7 5,471,196 - Pf1N1B4_4935 0.50 -0.9 5,471,196 - Pf1N1B4_4935 0.50 -0.3 5,471,548 - Pf1N1B4_4935 0.66 -1.0 5,471,756 + Pf1N1B4_4935 0.75 -0.1 5,471,831 + Pf1N1B4_4935 0.78 -1.2 5,471,832 - Pf1N1B4_4935 0.78 -1.2 5,471,977 + Pf1N1B4_4935 0.85 -1.3 5,471,977 + Pf1N1B4_4935 0.85 +0.4 5,472,149 + +1.0 5,472,150 - +1.2 5,472,150 - +0.1 5,472,457 - +1.1 5,472,475 + -0.6 5,472,597 + +0.4 5,472,649 + Pf1N1B4_4936 0.21 -0.8 5,472,649 + Pf1N1B4_4936 0.21 +0.2 5,472,662 - Pf1N1B4_4936 0.23 -2.1 5,473,135 + Pf1N1B4_4936 0.75 -0.1 5,473,135 + Pf1N1B4_4936 0.75 -0.1 5,473,187 - -0.6 5,473,187 - Pf1N1B4_4936 0.81 -1.1 5,473,188 + Pf1N1B4_4936 0.81 +0.8 5,473,188 + Pf1N1B4_4936 0.81 -0.3 5,473,188 + Pf1N1B4_4936 0.81 -0.6 5,473,189 - Pf1N1B4_4936 0.81 +0.4 5,473,225 + Pf1N1B4_4936 0.85 -0.2 5,473,226 - Pf1N1B4_4936 0.85 +0.4 5,473,226 - Pf1N1B4_4936 0.85 +0.8 5,473,292 - +0.5 5,473,318 + +1.0 5,473,318 + +0.0
Or see this region's nucleotide sequence