Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4248 and Pf1N1B4_4249 are separated by 111 nucleotides Pf1N1B4_4249 and Pf1N1B4_4250 are separated by 126 nucleotides
Pf1N1B4_4248 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 4,710,099 to 4,711,202
_4248
Pf1N1B4_4249 - transcriptional regulator, LuxR family, at 4,711,314 to 4,712,087
_4249
Pf1N1B4_4250 - Methyltransferase (EC 2.1.1.-), at 4,712,214 to 4,713,116
_4250
Position (kb)
4711
4712
4713 Strain fitness (log2 ratio)
-1
0
1
2 at 4710.320 kb on - strand, within Pf1N1B4_4248 at 4710.544 kb on + strand, within Pf1N1B4_4248 at 4710.593 kb on - strand at 4710.823 kb on + strand, within Pf1N1B4_4248 at 4711.090 kb on + strand, within Pf1N1B4_4248 at 4711.097 kb on - strand at 4711.551 kb on + strand, within Pf1N1B4_4249 at 4711.551 kb on + strand, within Pf1N1B4_4249 at 4711.650 kb on + strand, within Pf1N1B4_4249 at 4711.650 kb on + strand, within Pf1N1B4_4249 at 4711.650 kb on + strand, within Pf1N1B4_4249 at 4711.650 kb on + strand, within Pf1N1B4_4249 at 4711.653 kb on + strand, within Pf1N1B4_4249 at 4711.653 kb on + strand, within Pf1N1B4_4249 at 4711.653 kb on + strand, within Pf1N1B4_4249 at 4711.662 kb on + strand at 4711.663 kb on - strand, within Pf1N1B4_4249 at 4711.663 kb on - strand, within Pf1N1B4_4249 at 4711.985 kb on + strand, within Pf1N1B4_4249 at 4711.985 kb on + strand, within Pf1N1B4_4249 at 4712.036 kb on + strand at 4712.036 kb on + strand at 4712.036 kb on + strand at 4712.037 kb on - strand at 4712.037 kb on - strand at 4712.123 kb on + strand at 4712.123 kb on + strand at 4712.123 kb on + strand at 4712.123 kb on + strand at 4712.150 kb on - strand at 4712.370 kb on - strand, within Pf1N1B4_4250 at 4712.370 kb on - strand, within Pf1N1B4_4250 at 4712.370 kb on - strand, within Pf1N1B4_4250 at 4712.684 kb on - strand, within Pf1N1B4_4250 at 4712.875 kb on + strand, within Pf1N1B4_4250
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 4,710,320 - Pf1N1B4_4248 0.20 +1.3 4,710,544 + Pf1N1B4_4248 0.40 -0.2 4,710,593 - -1.0 4,710,823 + Pf1N1B4_4248 0.66 +0.1 4,711,090 + Pf1N1B4_4248 0.90 +0.7 4,711,097 - -0.3 4,711,551 + Pf1N1B4_4249 0.31 -1.2 4,711,551 + Pf1N1B4_4249 0.31 -1.1 4,711,650 + Pf1N1B4_4249 0.43 -1.2 4,711,650 + Pf1N1B4_4249 0.43 -1.1 4,711,650 + Pf1N1B4_4249 0.43 -0.5 4,711,650 + Pf1N1B4_4249 0.43 -1.7 4,711,653 + Pf1N1B4_4249 0.44 +1.5 4,711,653 + Pf1N1B4_4249 0.44 -0.2 4,711,653 + Pf1N1B4_4249 0.44 -1.1 4,711,662 + -0.5 4,711,663 - Pf1N1B4_4249 0.45 -0.5 4,711,663 - Pf1N1B4_4249 0.45 -1.5 4,711,985 + Pf1N1B4_4249 0.87 +0.1 4,711,985 + Pf1N1B4_4249 0.87 -1.2 4,712,036 + +1.2 4,712,036 + -0.5 4,712,036 + -0.5 4,712,037 - -1.5 4,712,037 - +0.5 4,712,123 + +0.2 4,712,123 + +1.9 4,712,123 + +2.1 4,712,123 + -0.5 4,712,150 - +1.9 4,712,370 - Pf1N1B4_4250 0.17 -0.5 4,712,370 - Pf1N1B4_4250 0.17 +0.3 4,712,370 - Pf1N1B4_4250 0.17 +0.5 4,712,684 - Pf1N1B4_4250 0.52 +0.1 4,712,875 + Pf1N1B4_4250 0.73 +0.8
Or see this region's nucleotide sequence