Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4101 and Pf1N1B4_4102 are separated by 96 nucleotides Pf1N1B4_4102 and Pf1N1B4_4103 are separated by 284 nucleotides
Pf1N1B4_4101 - Transcriptional regulator, AraC family, at 4,549,784 to 4,550,575
_4101
Pf1N1B4_4102 - Mannose-6-phosphate isomerase (EC 5.3.1.8), at 4,550,672 to 4,551,058
_4102
Pf1N1B4_4103 - Flagellar synthesis regulator FleN, at 4,551,343 to 4,552,164
_4103
Position (kb)
4550
4551
4552 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4549.877 kb on - strand, within Pf1N1B4_4101 at 4549.881 kb on - strand, within Pf1N1B4_4101 at 4549.909 kb on - strand, within Pf1N1B4_4101 at 4550.033 kb on + strand, within Pf1N1B4_4101 at 4550.033 kb on + strand, within Pf1N1B4_4101 at 4550.034 kb on - strand, within Pf1N1B4_4101 at 4550.061 kb on + strand, within Pf1N1B4_4101 at 4550.062 kb on - strand, within Pf1N1B4_4101 at 4550.099 kb on - strand at 4550.099 kb on - strand, within Pf1N1B4_4101 at 4550.099 kb on - strand, within Pf1N1B4_4101 at 4550.099 kb on - strand, within Pf1N1B4_4101 at 4550.099 kb on - strand, within Pf1N1B4_4101 at 4550.099 kb on - strand at 4550.121 kb on - strand, within Pf1N1B4_4101 at 4550.381 kb on + strand, within Pf1N1B4_4101 at 4550.382 kb on - strand, within Pf1N1B4_4101 at 4550.387 kb on - strand, within Pf1N1B4_4101 at 4550.402 kb on + strand, within Pf1N1B4_4101 at 4550.402 kb on + strand, within Pf1N1B4_4101 at 4550.402 kb on + strand, within Pf1N1B4_4101 at 4550.403 kb on - strand, within Pf1N1B4_4101 at 4550.403 kb on - strand, within Pf1N1B4_4101 at 4550.590 kb on + strand at 4550.630 kb on + strand at 4550.921 kb on + strand, within Pf1N1B4_4102 at 4550.921 kb on + strand, within Pf1N1B4_4102 at 4550.921 kb on + strand, within Pf1N1B4_4102 at 4550.922 kb on - strand, within Pf1N1B4_4102 at 4550.922 kb on - strand, within Pf1N1B4_4102 at 4550.979 kb on + strand, within Pf1N1B4_4102 at 4551.220 kb on + strand at 4551.461 kb on + strand, within Pf1N1B4_4103 at 4551.462 kb on - strand, within Pf1N1B4_4103 at 4551.462 kb on - strand, within Pf1N1B4_4103 at 4551.499 kb on - strand, within Pf1N1B4_4103 at 4551.653 kb on - strand at 4551.675 kb on + strand, within Pf1N1B4_4103
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 4,549,877 - Pf1N1B4_4101 0.12 +0.1 4,549,881 - Pf1N1B4_4101 0.12 +2.0 4,549,909 - Pf1N1B4_4101 0.16 +1.1 4,550,033 + Pf1N1B4_4101 0.31 -1.9 4,550,033 + Pf1N1B4_4101 0.31 -1.1 4,550,034 - Pf1N1B4_4101 0.32 +0.8 4,550,061 + Pf1N1B4_4101 0.35 -1.3 4,550,062 - Pf1N1B4_4101 0.35 -0.3 4,550,099 - +1.2 4,550,099 - Pf1N1B4_4101 0.40 -0.3 4,550,099 - Pf1N1B4_4101 0.40 +0.3 4,550,099 - Pf1N1B4_4101 0.40 +0.1 4,550,099 - Pf1N1B4_4101 0.40 -0.3 4,550,099 - +0.2 4,550,121 - Pf1N1B4_4101 0.43 +1.1 4,550,381 + Pf1N1B4_4101 0.75 -0.9 4,550,382 - Pf1N1B4_4101 0.76 +1.2 4,550,387 - Pf1N1B4_4101 0.76 +0.9 4,550,402 + Pf1N1B4_4101 0.78 -0.1 4,550,402 + Pf1N1B4_4101 0.78 +0.8 4,550,402 + Pf1N1B4_4101 0.78 -0.1 4,550,403 - Pf1N1B4_4101 0.78 -3.2 4,550,403 - Pf1N1B4_4101 0.78 -0.1 4,550,590 + +0.1 4,550,630 + -1.8 4,550,921 + Pf1N1B4_4102 0.64 -0.3 4,550,921 + Pf1N1B4_4102 0.64 -0.9 4,550,921 + Pf1N1B4_4102 0.64 -0.6 4,550,922 - Pf1N1B4_4102 0.65 +0.5 4,550,922 - Pf1N1B4_4102 0.65 -0.9 4,550,979 + Pf1N1B4_4102 0.79 -0.0 4,551,220 + +0.7 4,551,461 + Pf1N1B4_4103 0.14 +0.1 4,551,462 - Pf1N1B4_4103 0.14 +0.9 4,551,462 - Pf1N1B4_4103 0.14 -0.4 4,551,499 - Pf1N1B4_4103 0.19 -1.9 4,551,653 - -0.9 4,551,675 + Pf1N1B4_4103 0.40 +1.5
Or see this region's nucleotide sequence