Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2649 and Pf1N1B4_2650 are separated by 47 nucleotides Pf1N1B4_2650 and Pf1N1B4_2651 are separated by 31 nucleotides Pf1N1B4_2651 and Pf1N1B4_2652 are separated by 91 nucleotides Pf1N1B4_2652 and Pf1N1B4_2653 overlap by 4 nucleotides
Pf1N1B4_2649 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 2,986,677 to 2,987,810
_2649
Pf1N1B4_2650 - Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9), at 2,987,858 to 2,988,520
_2650
Pf1N1B4_2651 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25), at 2,988,552 to 2,989,643
_2651
Pf1N1B4_2652 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 2,989,735 to 2,990,211
_2652
Pf1N1B4_2653 - Transcription termination protein NusB, at 2,990,208 to 2,990,708
_2653
Position (kb)
2988
2989
2990 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2987.806 kb on + strand at 2987.806 kb on + strand at 2987.806 kb on + strand at 2987.807 kb on - strand at 2987.807 kb on - strand at 2987.807 kb on - strand at 2987.808 kb on + strand at 2987.808 kb on + strand at 2987.808 kb on + strand at 2987.808 kb on + strand at 2988.563 kb on + strand at 2988.845 kb on + strand, within Pf1N1B4_2651 at 2989.163 kb on + strand, within Pf1N1B4_2651 at 2989.163 kb on + strand, within Pf1N1B4_2651 at 2989.163 kb on + strand, within Pf1N1B4_2651 at 2989.227 kb on + strand, within Pf1N1B4_2651 at 2989.227 kb on + strand, within Pf1N1B4_2651 at 2989.228 kb on - strand, within Pf1N1B4_2651 at 2989.228 kb on - strand, within Pf1N1B4_2651 at 2989.447 kb on + strand, within Pf1N1B4_2651 at 2989.447 kb on + strand, within Pf1N1B4_2651 at 2989.447 kb on + strand, within Pf1N1B4_2651 at 2989.447 kb on + strand, within Pf1N1B4_2651 at 2989.447 kb on + strand, within Pf1N1B4_2651 at 2989.447 kb on + strand at 2989.447 kb on + strand, within Pf1N1B4_2651 at 2989.447 kb on + strand, within Pf1N1B4_2651 at 2989.447 kb on + strand, within Pf1N1B4_2651 at 2989.506 kb on + strand, within Pf1N1B4_2651 at 2989.506 kb on + strand, within Pf1N1B4_2651 at 2989.506 kb on + strand, within Pf1N1B4_2651 at 2989.506 kb on + strand, within Pf1N1B4_2651 at 2989.506 kb on + strand, within Pf1N1B4_2651 at 2989.589 kb on + strand at 2989.621 kb on + strand at 2989.626 kb on + strand at 2989.626 kb on + strand at 2989.626 kb on + strand at 2989.626 kb on + strand at 2989.698 kb on + strand at 2989.698 kb on + strand at 2989.720 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 2,987,806 + -1.2 2,987,806 + +1.3 2,987,806 + +2.7 2,987,807 - +0.6 2,987,807 - -3.1 2,987,807 - +0.3 2,987,808 + +1.3 2,987,808 + +0.3 2,987,808 + -0.7 2,987,808 + -0.3 2,988,563 + -0.3 2,988,845 + Pf1N1B4_2651 0.27 +0.3 2,989,163 + Pf1N1B4_2651 0.56 +1.4 2,989,163 + Pf1N1B4_2651 0.56 -0.4 2,989,163 + Pf1N1B4_2651 0.56 -2.4 2,989,227 + Pf1N1B4_2651 0.62 -2.0 2,989,227 + Pf1N1B4_2651 0.62 +0.8 2,989,228 - Pf1N1B4_2651 0.62 -0.3 2,989,228 - Pf1N1B4_2651 0.62 +0.3 2,989,447 + Pf1N1B4_2651 0.82 -1.2 2,989,447 + Pf1N1B4_2651 0.82 -0.7 2,989,447 + Pf1N1B4_2651 0.82 -0.3 2,989,447 + Pf1N1B4_2651 0.82 +0.8 2,989,447 + Pf1N1B4_2651 0.82 -1.2 2,989,447 + -0.7 2,989,447 + Pf1N1B4_2651 0.82 -0.3 2,989,447 + Pf1N1B4_2651 0.82 +0.3 2,989,447 + Pf1N1B4_2651 0.82 -1.3 2,989,506 + Pf1N1B4_2651 0.87 -0.7 2,989,506 + Pf1N1B4_2651 0.87 +0.3 2,989,506 + Pf1N1B4_2651 0.87 -0.7 2,989,506 + Pf1N1B4_2651 0.87 +0.5 2,989,506 + Pf1N1B4_2651 0.87 +0.3 2,989,589 + +1.3 2,989,621 + +0.3 2,989,626 + +1.6 2,989,626 + +0.5 2,989,626 + +0.3 2,989,626 + +0.3 2,989,698 + +0.3 2,989,698 + -1.1 2,989,720 + +0.7
Or see this region's nucleotide sequence