Experiment: D-Glucose 20 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06307 and H281DRAFT_06308 are separated by 96 nucleotides H281DRAFT_06308 and H281DRAFT_06309 are separated by 420 nucleotides H281DRAFT_06309 and H281DRAFT_06310 are separated by 16 nucleotides
H281DRAFT_06307: H281DRAFT_06307 - thiamine-phosphate kinase, at 89,725 to 90,723
_06307
H281DRAFT_06308: H281DRAFT_06308 - malate dehydrogenase (oxaloacetate-decarboxylating)(NADP+), at 90,820 to 93,159
_06308
H281DRAFT_06309: H281DRAFT_06309 - ribonuclease T1, at 93,580 to 93,996
_06309
H281DRAFT_06310: H281DRAFT_06310 - Barstar (barnase inhibitor), at 94,013 to 94,573
_06310
Position (kb) 90
91
92
93
94 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 90.759 kb on - strand at 90.759 kb on - strand at 90.817 kb on - strand at 90.817 kb on - strand at 90.841 kb on - strand at 90.864 kb on + strand at 90.911 kb on - strand at 91.156 kb on - strand, within H281DRAFT_06308 at 91.973 kb on - strand, within H281DRAFT_06308 at 92.120 kb on - strand, within H281DRAFT_06308 at 92.146 kb on + strand, within H281DRAFT_06308 at 92.146 kb on + strand, within H281DRAFT_06308 at 92.422 kb on + strand, within H281DRAFT_06308 at 92.425 kb on + strand, within H281DRAFT_06308 at 92.426 kb on - strand at 92.580 kb on + strand at 92.621 kb on - strand, within H281DRAFT_06308 at 92.832 kb on + strand at 93.001 kb on + strand at 93.002 kb on - strand at 93.170 kb on - strand at 93.208 kb on + strand at 93.397 kb on + strand at 93.404 kb on - strand at 93.522 kb on + strand at 93.546 kb on - strand at 93.559 kb on + strand at 93.560 kb on - strand at 93.560 kb on - strand at 93.625 kb on - strand, within H281DRAFT_06309 at 93.625 kb on - strand, within H281DRAFT_06309 at 93.676 kb on + strand at 93.753 kb on + strand, within H281DRAFT_06309 at 93.753 kb on + strand, within H281DRAFT_06309 at 93.753 kb on + strand, within H281DRAFT_06309 at 93.753 kb on + strand at 93.753 kb on + strand, within H281DRAFT_06309 at 93.754 kb on - strand, within H281DRAFT_06309 at 93.754 kb on - strand, within H281DRAFT_06309 at 93.754 kb on - strand, within H281DRAFT_06309 at 93.754 kb on - strand, within H281DRAFT_06309 at 93.754 kb on - strand, within H281DRAFT_06309 at 93.754 kb on - strand, within H281DRAFT_06309 at 93.754 kb on - strand, within H281DRAFT_06309 at 93.784 kb on + strand at 93.865 kb on + strand, within H281DRAFT_06309 at 93.865 kb on + strand, within H281DRAFT_06309 at 93.865 kb on + strand, within H281DRAFT_06309 at 93.865 kb on + strand, within H281DRAFT_06309 at 93.866 kb on - strand, within H281DRAFT_06309 at 93.866 kb on - strand, within H281DRAFT_06309 at 93.892 kb on - strand, within H281DRAFT_06309 at 93.949 kb on + strand, within H281DRAFT_06309 at 93.953 kb on - strand, within H281DRAFT_06309 at 93.967 kb on + strand at 93.968 kb on - strand at 93.968 kb on - strand at 93.968 kb on - strand at 93.979 kb on - strand at 93.984 kb on + strand at 93.984 kb on + strand at 93.984 kb on + strand at 94.028 kb on + strand at 94.028 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose 20 mM (C) remove 90,759 - -0.9 90,759 - -0.3 90,817 - +0.4 90,817 - +0.4 90,841 - +0.1 90,864 + +0.5 90,911 - +1.6 91,156 - H281DRAFT_06308 0.14 +0.7 91,973 - H281DRAFT_06308 0.49 +3.2 92,120 - H281DRAFT_06308 0.56 +0.7 92,146 + H281DRAFT_06308 0.57 +0.1 92,146 + H281DRAFT_06308 0.57 +0.3 92,422 + H281DRAFT_06308 0.68 -0.7 92,425 + H281DRAFT_06308 0.69 +1.9 92,426 - +3.9 92,580 + +0.1 92,621 - H281DRAFT_06308 0.77 +0.1 92,832 + +1.8 93,001 + +3.4 93,002 - -1.0 93,170 - -1.0 93,208 + +0.0 93,397 + +0.3 93,404 - -0.0 93,522 + -0.5 93,546 - -1.4 93,559 + -0.2 93,560 - +2.1 93,560 - -0.5 93,625 - H281DRAFT_06309 0.11 -1.1 93,625 - H281DRAFT_06309 0.11 -1.5 93,676 + +1.1 93,753 + H281DRAFT_06309 0.41 -0.2 93,753 + H281DRAFT_06309 0.41 +0.2 93,753 + H281DRAFT_06309 0.41 -1.4 93,753 + +0.1 93,753 + H281DRAFT_06309 0.41 -0.9 93,754 - H281DRAFT_06309 0.42 +0.9 93,754 - H281DRAFT_06309 0.42 -2.5 93,754 - H281DRAFT_06309 0.42 -1.4 93,754 - H281DRAFT_06309 0.42 +1.1 93,754 - H281DRAFT_06309 0.42 -0.5 93,754 - H281DRAFT_06309 0.42 +0.4 93,754 - H281DRAFT_06309 0.42 -0.4 93,784 + +1.1 93,865 + H281DRAFT_06309 0.68 -1.3 93,865 + H281DRAFT_06309 0.68 -0.8 93,865 + H281DRAFT_06309 0.68 -0.1 93,865 + H281DRAFT_06309 0.68 -0.3 93,866 - H281DRAFT_06309 0.69 -1.3 93,866 - H281DRAFT_06309 0.69 -0.5 93,892 - H281DRAFT_06309 0.75 -0.6 93,949 + H281DRAFT_06309 0.88 -0.2 93,953 - H281DRAFT_06309 0.89 -0.9 93,967 + -0.2 93,968 - -0.9 93,968 - -0.2 93,968 - +0.6 93,979 - -1.6 93,984 + +0.7 93,984 + +0.6 93,984 + +0.5 94,028 + -0.3 94,028 + -1.2
Or see this region's nucleotide sequence