Experiment: D-Glucose 20 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05717 and H281DRAFT_05718 are separated by 256 nucleotides H281DRAFT_05718 and H281DRAFT_05719 are separated by 228 nucleotides H281DRAFT_05719 and H281DRAFT_05720 are separated by 108 nucleotides
H281DRAFT_05717: H281DRAFT_05717 - ribulose-5-phosphate 3-epimerase, at 136,211 to 136,927
_05717
H281DRAFT_05718: H281DRAFT_05718 - ApaG protein, at 137,184 to 137,558
_05718
H281DRAFT_05719: H281DRAFT_05719 - membrane-bound lytic murein transglycosylase A, at 137,787 to 138,941
_05719
H281DRAFT_05720: H281DRAFT_05720 - phenylacetate-CoA ligase, at 139,050 to 140,354
_05720
Position (kb) 137
138
139 Strain fitness (log2 ratio) -2
-1
0
1
2 at 136.930 kb on + strand at 137.028 kb on - strand at 137.048 kb on + strand at 137.048 kb on + strand at 137.048 kb on + strand at 137.049 kb on - strand at 137.049 kb on - strand at 137.049 kb on - strand at 137.049 kb on - strand at 137.049 kb on - strand at 137.273 kb on + strand, within H281DRAFT_05718 at 137.273 kb on + strand, within H281DRAFT_05718 at 137.273 kb on + strand, within H281DRAFT_05718 at 137.274 kb on - strand, within H281DRAFT_05718 at 137.425 kb on - strand, within H281DRAFT_05718 at 137.426 kb on + strand, within H281DRAFT_05718 at 137.427 kb on - strand, within H281DRAFT_05718 at 137.427 kb on - strand, within H281DRAFT_05718 at 137.427 kb on - strand, within H281DRAFT_05718 at 137.427 kb on - strand, within H281DRAFT_05718 at 137.475 kb on + strand, within H281DRAFT_05718 at 137.475 kb on + strand at 137.475 kb on + strand, within H281DRAFT_05718 at 137.475 kb on + strand, within H281DRAFT_05718 at 137.475 kb on + strand, within H281DRAFT_05718 at 137.476 kb on - strand, within H281DRAFT_05718 at 137.476 kb on - strand, within H281DRAFT_05718 at 137.476 kb on - strand, within H281DRAFT_05718 at 137.592 kb on + strand at 137.593 kb on - strand at 137.801 kb on + strand at 137.816 kb on + strand at 139.051 kb on + strand at 139.169 kb on - strand at 139.169 kb on - strand at 139.197 kb on + strand, within H281DRAFT_05720 at 139.275 kb on + strand, within H281DRAFT_05720 at 139.275 kb on + strand, within H281DRAFT_05720 at 139.276 kb on - strand, within H281DRAFT_05720 at 139.285 kb on + strand, within H281DRAFT_05720 at 139.286 kb on - strand, within H281DRAFT_05720 at 139.286 kb on - strand, within H281DRAFT_05720 at 139.286 kb on - strand, within H281DRAFT_05720 at 139.397 kb on + strand at 139.398 kb on - strand, within H281DRAFT_05720 at 139.559 kb on - strand, within H281DRAFT_05720 at 139.645 kb on + strand, within H281DRAFT_05720 at 139.645 kb on + strand, within H281DRAFT_05720 at 139.646 kb on - strand, within H281DRAFT_05720 at 139.646 kb on - strand, within H281DRAFT_05720 at 139.646 kb on - strand, within H281DRAFT_05720 at 139.646 kb on - strand, within H281DRAFT_05720 at 139.646 kb on - strand, within H281DRAFT_05720 at 139.728 kb on + strand, within H281DRAFT_05720 at 139.728 kb on + strand, within H281DRAFT_05720 at 139.728 kb on + strand, within H281DRAFT_05720 at 139.729 kb on - strand, within H281DRAFT_05720 at 139.729 kb on - strand, within H281DRAFT_05720 at 139.795 kb on + strand, within H281DRAFT_05720 at 139.795 kb on + strand, within H281DRAFT_05720 at 139.795 kb on + strand, within H281DRAFT_05720 at 139.796 kb on - strand, within H281DRAFT_05720 at 139.796 kb on - strand, within H281DRAFT_05720 at 139.796 kb on - strand, within H281DRAFT_05720 at 139.909 kb on + strand, within H281DRAFT_05720 at 139.910 kb on - strand, within H281DRAFT_05720
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose 20 mM (C) remove 136,930 + +0.7 137,028 - -0.2 137,048 + -0.2 137,048 + +1.0 137,048 + +1.8 137,049 - +1.1 137,049 - -0.6 137,049 - -1.0 137,049 - +0.4 137,049 - -0.2 137,273 + H281DRAFT_05718 0.24 +0.2 137,273 + H281DRAFT_05718 0.24 -0.9 137,273 + H281DRAFT_05718 0.24 +0.6 137,274 - H281DRAFT_05718 0.24 -0.9 137,425 - H281DRAFT_05718 0.64 -0.4 137,426 + H281DRAFT_05718 0.65 +0.1 137,427 - H281DRAFT_05718 0.65 +1.1 137,427 - H281DRAFT_05718 0.65 -0.1 137,427 - H281DRAFT_05718 0.65 -1.2 137,427 - H281DRAFT_05718 0.65 -0.4 137,475 + H281DRAFT_05718 0.78 -0.1 137,475 + +0.4 137,475 + H281DRAFT_05718 0.78 +1.3 137,475 + H281DRAFT_05718 0.78 -0.4 137,475 + H281DRAFT_05718 0.78 +0.3 137,476 - H281DRAFT_05718 0.78 +0.3 137,476 - H281DRAFT_05718 0.78 +0.4 137,476 - H281DRAFT_05718 0.78 -0.3 137,592 + +0.4 137,593 - -1.9 137,801 + +0.1 137,816 + -0.0 139,051 + +1.3 139,169 - -0.2 139,169 - +0.4 139,197 + H281DRAFT_05720 0.11 +0.1 139,275 + H281DRAFT_05720 0.17 +0.6 139,275 + H281DRAFT_05720 0.17 +1.1 139,276 - H281DRAFT_05720 0.17 +0.3 139,285 + H281DRAFT_05720 0.18 -0.4 139,286 - H281DRAFT_05720 0.18 +1.1 139,286 - H281DRAFT_05720 0.18 +0.3 139,286 - H281DRAFT_05720 0.18 +0.7 139,397 + +2.1 139,398 - H281DRAFT_05720 0.27 +0.6 139,559 - H281DRAFT_05720 0.39 -0.7 139,645 + H281DRAFT_05720 0.46 +0.3 139,645 + H281DRAFT_05720 0.46 -0.4 139,646 - H281DRAFT_05720 0.46 +0.4 139,646 - H281DRAFT_05720 0.46 +0.0 139,646 - H281DRAFT_05720 0.46 -1.7 139,646 - H281DRAFT_05720 0.46 +0.2 139,646 - H281DRAFT_05720 0.46 +0.8 139,728 + H281DRAFT_05720 0.52 +0.4 139,728 + H281DRAFT_05720 0.52 +0.3 139,728 + H281DRAFT_05720 0.52 +0.0 139,729 - H281DRAFT_05720 0.52 +0.7 139,729 - H281DRAFT_05720 0.52 +0.5 139,795 + H281DRAFT_05720 0.57 +0.7 139,795 + H281DRAFT_05720 0.57 +0.6 139,795 + H281DRAFT_05720 0.57 +0.1 139,796 - H281DRAFT_05720 0.57 +0.3 139,796 - H281DRAFT_05720 0.57 +0.5 139,796 - H281DRAFT_05720 0.57 +1.2 139,909 + H281DRAFT_05720 0.66 +0.1 139,910 - H281DRAFT_05720 0.66 +1.1
Or see this region's nucleotide sequence