Experiment: D-Glucose 20 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01593 and H281DRAFT_01594 overlap by 4 nucleotides H281DRAFT_01594 and H281DRAFT_01595 are separated by 5 nucleotides H281DRAFT_01595 and H281DRAFT_01596 are separated by 106 nucleotides H281DRAFT_01596 and H281DRAFT_01597 are separated by 13 nucleotides
H281DRAFT_01593: H281DRAFT_01593 - 4-carboxymuconolactone decarboxylase, at 472,270 to 472,662
_01593
H281DRAFT_01594: H281DRAFT_01594 - 3-oxoadipate enol-lactonase, at 472,659 to 473,450
_01594
H281DRAFT_01595: H281DRAFT_01595 - 3-carboxy-cis,cis-muconate cycloisomerase, at 473,456 to 474,835
_01595
H281DRAFT_01596: H281DRAFT_01596 - 3-oxoadipate CoA-transferase beta subunit, at 474,942 to 475,601
_01596
H281DRAFT_01597: H281DRAFT_01597 - 3-oxoadipate CoA-transferase alpha subunit, at 475,615 to 476,322
_01597
Position (kb)
473
474
475 Strain fitness (log2 ratio)
-1
0
1
2 at 472.727 kb on + strand at 472.904 kb on - strand, within H281DRAFT_01594 at 472.929 kb on + strand, within H281DRAFT_01594 at 473.444 kb on - strand at 473.517 kb on + strand at 473.517 kb on + strand at 473.517 kb on + strand at 473.518 kb on - strand at 473.538 kb on + strand at 473.912 kb on + strand, within H281DRAFT_01595 at 474.474 kb on - strand, within H281DRAFT_01595 at 474.531 kb on + strand, within H281DRAFT_01595 at 474.734 kb on + strand at 474.735 kb on - strand at 475.121 kb on + strand, within H281DRAFT_01596 at 475.122 kb on - strand, within H281DRAFT_01596 at 475.122 kb on - strand, within H281DRAFT_01596 at 475.177 kb on + strand, within H281DRAFT_01596 at 475.538 kb on - strand at 475.809 kb on + strand, within H281DRAFT_01597
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose 20 mM (C) remove 472,727 + +0.3 472,904 - H281DRAFT_01594 0.31 -0.8 472,929 + H281DRAFT_01594 0.34 +0.3 473,444 - +0.2 473,517 + +0.7 473,517 + +0.6 473,517 + -1.0 473,518 - +1.5 473,538 + +0.2 473,912 + H281DRAFT_01595 0.33 +0.4 474,474 - H281DRAFT_01595 0.74 +0.3 474,531 + H281DRAFT_01595 0.78 +0.9 474,734 + +0.4 474,735 - -0.8 475,121 + H281DRAFT_01596 0.27 +1.8 475,122 - H281DRAFT_01596 0.27 +0.4 475,122 - H281DRAFT_01596 0.27 -0.0 475,177 + H281DRAFT_01596 0.36 -0.5 475,538 - +0.2 475,809 + H281DRAFT_01597 0.27 -0.2
Or see this region's nucleotide sequence