Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_01595

Experiment: D-Glucose 20 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_01593 and H281DRAFT_01594 overlap by 4 nucleotidesH281DRAFT_01594 and H281DRAFT_01595 are separated by 5 nucleotidesH281DRAFT_01595 and H281DRAFT_01596 are separated by 106 nucleotidesH281DRAFT_01596 and H281DRAFT_01597 are separated by 13 nucleotides H281DRAFT_01593: H281DRAFT_01593 - 4-carboxymuconolactone decarboxylase, at 472,270 to 472,662 _01593 H281DRAFT_01594: H281DRAFT_01594 - 3-oxoadipate enol-lactonase, at 472,659 to 473,450 _01594 H281DRAFT_01595: H281DRAFT_01595 - 3-carboxy-cis,cis-muconate cycloisomerase, at 473,456 to 474,835 _01595 H281DRAFT_01596: H281DRAFT_01596 - 3-oxoadipate CoA-transferase beta subunit, at 474,942 to 475,601 _01596 H281DRAFT_01597: H281DRAFT_01597 - 3-oxoadipate CoA-transferase alpha subunit, at 475,615 to 476,322 _01597 Position (kb) 473 474 475Strain fitness (log2 ratio) -1 0 1 2at 472.727 kb on + strandat 472.904 kb on - strand, within H281DRAFT_01594at 472.929 kb on + strand, within H281DRAFT_01594at 473.444 kb on - strandat 473.517 kb on + strandat 473.517 kb on + strandat 473.517 kb on + strandat 473.518 kb on - strandat 473.538 kb on + strandat 473.912 kb on + strand, within H281DRAFT_01595at 474.474 kb on - strand, within H281DRAFT_01595at 474.531 kb on + strand, within H281DRAFT_01595at 474.734 kb on + strandat 474.735 kb on - strandat 475.121 kb on + strand, within H281DRAFT_01596at 475.122 kb on - strand, within H281DRAFT_01596at 475.122 kb on - strand, within H281DRAFT_01596at 475.177 kb on + strand, within H281DRAFT_01596at 475.538 kb on - strandat 475.809 kb on + strand, within H281DRAFT_01597

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose 20 mM (C)
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472,727 + +0.3
472,904 - H281DRAFT_01594 0.31 -0.8
472,929 + H281DRAFT_01594 0.34 +0.3
473,444 - +0.2
473,517 + +0.7
473,517 + +0.6
473,517 + -1.0
473,518 - +1.5
473,538 + +0.2
473,912 + H281DRAFT_01595 0.33 +0.4
474,474 - H281DRAFT_01595 0.74 +0.3
474,531 + H281DRAFT_01595 0.78 +0.9
474,734 + +0.4
474,735 - -0.8
475,121 + H281DRAFT_01596 0.27 +1.8
475,122 - H281DRAFT_01596 0.27 +0.4
475,122 - H281DRAFT_01596 0.27 -0.0
475,177 + H281DRAFT_01596 0.36 -0.5
475,538 - +0.2
475,809 + H281DRAFT_01597 0.27 -0.2

Or see this region's nucleotide sequence