Experiment: ICP1_phage_0.23_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_09235 and CSW01_09240 are separated by 419 nucleotides CSW01_09240 and CSW01_09245 are separated by 55 nucleotides CSW01_09245 and CSW01_09250 are separated by 96 nucleotides
CSW01_09235: CSW01_09235 - cytochrome d terminal oxidase subunit 1, at 1,981,613 to 1,983,238
_09235
CSW01_09240: CSW01_09240 - Holliday junction branch migration DNA helicase RuvB, at 1,983,658 to 1,984,662
_09240
CSW01_09245: CSW01_09245 - Holliday junction branch migration protein RuvA, at 1,984,718 to 1,985,332
_09245
CSW01_09250: CSW01_09250 - crossover junction endodeoxyribonuclease RuvC, at 1,985,429 to 1,985,950
_09250
Position (kb) 1983
1984
1985 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1983.400 kb on - strand at 1983.400 kb on - strand at 1983.402 kb on + strand at 1983.437 kb on + strand at 1983.470 kb on + strand at 1983.500 kb on - strand at 1983.870 kb on + strand, within CSW01_09240 at 1983.871 kb on + strand, within CSW01_09240 at 1983.934 kb on - strand, within CSW01_09240 at 1983.952 kb on - strand, within CSW01_09240 at 1984.023 kb on + strand, within CSW01_09240 at 1984.031 kb on - strand, within CSW01_09240 at 1984.072 kb on + strand, within CSW01_09240 at 1984.080 kb on - strand, within CSW01_09240 at 1984.146 kb on + strand, within CSW01_09240 at 1984.148 kb on + strand, within CSW01_09240 at 1984.154 kb on - strand, within CSW01_09240 at 1984.154 kb on - strand, within CSW01_09240 at 1984.156 kb on - strand, within CSW01_09240 at 1984.175 kb on - strand, within CSW01_09240 at 1984.276 kb on + strand, within CSW01_09240 at 1984.276 kb on + strand, within CSW01_09240 at 1984.393 kb on + strand, within CSW01_09240 at 1984.459 kb on - strand, within CSW01_09240 at 1984.723 kb on + strand at 1984.723 kb on + strand at 1984.731 kb on - strand at 1984.758 kb on - strand at 1984.809 kb on + strand, within CSW01_09245 at 1984.906 kb on - strand, within CSW01_09245 at 1984.906 kb on - strand, within CSW01_09245 at 1984.913 kb on - strand, within CSW01_09245 at 1984.913 kb on - strand, within CSW01_09245 at 1984.913 kb on - strand, within CSW01_09245 at 1984.940 kb on - strand, within CSW01_09245 at 1984.995 kb on + strand, within CSW01_09245 at 1985.012 kb on + strand, within CSW01_09245 at 1985.081 kb on - strand, within CSW01_09245 at 1985.100 kb on + strand, within CSW01_09245 at 1985.112 kb on + strand, within CSW01_09245 at 1985.139 kb on - strand, within CSW01_09245 at 1985.161 kb on - strand, within CSW01_09245 at 1985.176 kb on + strand, within CSW01_09245 at 1985.219 kb on - strand, within CSW01_09245 at 1985.462 kb on - strand at 1985.515 kb on + strand, within CSW01_09250 at 1985.538 kb on + strand, within CSW01_09250 at 1985.538 kb on + strand, within CSW01_09250 at 1985.546 kb on - strand, within CSW01_09250 at 1985.587 kb on - strand, within CSW01_09250
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_0.23_MOI_rep2 remove 1,983,400 - +0.5 1,983,400 - +0.2 1,983,402 + +0.8 1,983,437 + +0.9 1,983,470 + +1.4 1,983,500 - -0.1 1,983,870 + CSW01_09240 0.21 -1.2 1,983,871 + CSW01_09240 0.21 +0.6 1,983,934 - CSW01_09240 0.27 -0.3 1,983,952 - CSW01_09240 0.29 +0.1 1,984,023 + CSW01_09240 0.36 +1.6 1,984,031 - CSW01_09240 0.37 -0.8 1,984,072 + CSW01_09240 0.41 -0.8 1,984,080 - CSW01_09240 0.42 -1.8 1,984,146 + CSW01_09240 0.49 -2.3 1,984,148 + CSW01_09240 0.49 -1.5 1,984,154 - CSW01_09240 0.49 -2.3 1,984,154 - CSW01_09240 0.49 -3.1 1,984,156 - CSW01_09240 0.50 -1.8 1,984,175 - CSW01_09240 0.51 -2.4 1,984,276 + CSW01_09240 0.61 -0.3 1,984,276 + CSW01_09240 0.61 -1.3 1,984,393 + CSW01_09240 0.73 -2.6 1,984,459 - CSW01_09240 0.80 -1.5 1,984,723 + -1.0 1,984,723 + +2.1 1,984,731 - +0.2 1,984,758 - -2.7 1,984,809 + CSW01_09245 0.15 +0.6 1,984,906 - CSW01_09245 0.31 +1.4 1,984,906 - CSW01_09245 0.31 +1.0 1,984,913 - CSW01_09245 0.32 -0.9 1,984,913 - CSW01_09245 0.32 -1.7 1,984,913 - CSW01_09245 0.32 -2.5 1,984,940 - CSW01_09245 0.36 -3.3 1,984,995 + CSW01_09245 0.45 -2.0 1,985,012 + CSW01_09245 0.48 -2.0 1,985,081 - CSW01_09245 0.59 -1.6 1,985,100 + CSW01_09245 0.62 -0.2 1,985,112 + CSW01_09245 0.64 -2.8 1,985,139 - CSW01_09245 0.68 -1.0 1,985,161 - CSW01_09245 0.72 -0.0 1,985,176 + CSW01_09245 0.74 -2.7 1,985,219 - CSW01_09245 0.81 -0.9 1,985,462 - -0.4 1,985,515 + CSW01_09250 0.16 +0.2 1,985,538 + CSW01_09250 0.21 -1.2 1,985,538 + CSW01_09250 0.21 -0.0 1,985,546 - CSW01_09250 0.22 +0.1 1,985,587 - CSW01_09250 0.30 -1.2
Or see this region's nucleotide sequence