Experiment: ICP1_phage_0.23_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_08510 and CSW01_08515 are separated by 46 nucleotides CSW01_08515 and CSW01_08520 are separated by 379 nucleotides CSW01_08520 and CSW01_08525 are separated by 63 nucleotides CSW01_08525 and CSW01_08530 are separated by 167 nucleotides CSW01_08530 and CSW01_08535 are separated by 13 nucleotides CSW01_08535 and CSW01_08540 are separated by 103 nucleotides
CSW01_08510: CSW01_08510 - pentaheme c-type cytochrome TorC, at 1,825,939 to 1,827,123
_08510
CSW01_08515: CSW01_08515 - trimethylamine N-oxide reductase system protein TorE, at 1,827,170 to 1,827,352
_08515
CSW01_08520: CSW01_08520 - hypothetical protein, at 1,827,732 to 1,827,968
_08520
CSW01_08525: CSW01_08525 - formate transporter FocA, at 1,828,032 to 1,828,874
_08525
CSW01_08530: CSW01_08530 - RNA helicase, at 1,829,042 to 1,829,212
_08530
CSW01_08535: CSW01_08535 - TIGR02647 family protein, at 1,829,226 to 1,829,459
_08535
CSW01_08540: CSW01_08540 - NAD(P)-dependent oxidoreductase, at 1,829,563 to 1,830,393
_08540
Position (kb) 1828
1829 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1827.132 kb on - strand at 1827.217 kb on - strand, within CSW01_08515 at 1827.282 kb on + strand, within CSW01_08515 at 1827.292 kb on + strand, within CSW01_08515 at 1827.313 kb on - strand, within CSW01_08515 at 1827.475 kb on + strand at 1827.525 kb on - strand at 1827.709 kb on + strand at 1827.731 kb on + strand at 1827.770 kb on - strand, within CSW01_08520 at 1827.910 kb on - strand, within CSW01_08520 at 1827.987 kb on - strand at 1828.002 kb on + strand at 1828.013 kb on + strand at 1828.029 kb on + strand at 1828.068 kb on + strand at 1828.078 kb on - strand at 1828.102 kb on - strand at 1828.148 kb on - strand, within CSW01_08525 at 1828.228 kb on + strand, within CSW01_08525 at 1828.296 kb on - strand, within CSW01_08525 at 1828.298 kb on - strand, within CSW01_08525 at 1828.298 kb on - strand, within CSW01_08525 at 1828.333 kb on - strand, within CSW01_08525 at 1828.646 kb on - strand, within CSW01_08525 at 1828.663 kb on - strand, within CSW01_08525 at 1828.663 kb on - strand, within CSW01_08525 at 1828.698 kb on - strand, within CSW01_08525 at 1828.716 kb on - strand, within CSW01_08525 at 1828.870 kb on - strand at 1828.979 kb on - strand at 1829.049 kb on + strand at 1829.049 kb on + strand at 1829.049 kb on + strand at 1829.049 kb on + strand at 1829.057 kb on - strand at 1829.140 kb on + strand, within CSW01_08530 at 1829.148 kb on - strand, within CSW01_08530 at 1829.185 kb on + strand, within CSW01_08530 at 1829.185 kb on + strand, within CSW01_08530 at 1829.193 kb on - strand, within CSW01_08530 at 1829.193 kb on - strand, within CSW01_08530 at 1829.216 kb on + strand at 1829.285 kb on - strand, within CSW01_08535 at 1829.287 kb on + strand, within CSW01_08535 at 1829.339 kb on - strand, within CSW01_08535 at 1829.362 kb on - strand, within CSW01_08535 at 1829.475 kb on + strand at 1829.483 kb on - strand at 1829.535 kb on + strand at 1829.560 kb on - strand at 1829.570 kb on - strand at 1829.570 kb on - strand at 1829.633 kb on + strand at 1829.680 kb on - strand, within CSW01_08540 at 1829.686 kb on - strand, within CSW01_08540 at 1829.736 kb on + strand, within CSW01_08540 at 1829.804 kb on + strand, within CSW01_08540 at 1829.817 kb on - strand, within CSW01_08540 at 1829.827 kb on - strand, within CSW01_08540
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_0.23_MOI_rep2 remove 1,827,132 - -3.3 1,827,217 - CSW01_08515 0.26 -2.0 1,827,282 + CSW01_08515 0.61 +1.2 1,827,292 + CSW01_08515 0.67 -1.2 1,827,313 - CSW01_08515 0.78 -1.0 1,827,475 + -1.3 1,827,525 - -3.3 1,827,709 + +1.1 1,827,731 + -2.6 1,827,770 - CSW01_08520 0.16 +0.7 1,827,910 - CSW01_08520 0.75 -1.5 1,827,987 - +0.5 1,828,002 + -2.4 1,828,013 + +0.5 1,828,029 + -0.9 1,828,068 + -0.5 1,828,078 - -3.0 1,828,102 - +0.1 1,828,148 - CSW01_08525 0.14 -0.9 1,828,228 + CSW01_08525 0.23 +0.4 1,828,296 - CSW01_08525 0.31 -0.3 1,828,298 - CSW01_08525 0.32 -1.1 1,828,298 - CSW01_08525 0.32 +2.0 1,828,333 - CSW01_08525 0.36 +0.8 1,828,646 - CSW01_08525 0.73 -0.3 1,828,663 - CSW01_08525 0.75 +1.0 1,828,663 - CSW01_08525 0.75 +0.1 1,828,698 - CSW01_08525 0.79 +1.9 1,828,716 - CSW01_08525 0.81 +0.5 1,828,870 - -0.5 1,828,979 - -3.2 1,829,049 + -0.9 1,829,049 + +0.2 1,829,049 + -0.3 1,829,049 + -2.5 1,829,057 - +2.6 1,829,140 + CSW01_08530 0.57 +1.7 1,829,148 - CSW01_08530 0.62 +0.4 1,829,185 + CSW01_08530 0.84 +0.8 1,829,185 + CSW01_08530 0.84 +0.3 1,829,193 - CSW01_08530 0.88 -0.3 1,829,193 - CSW01_08530 0.88 -1.6 1,829,216 + -0.5 1,829,285 - CSW01_08535 0.25 +0.3 1,829,287 + CSW01_08535 0.26 -0.9 1,829,339 - CSW01_08535 0.48 +1.1 1,829,362 - CSW01_08535 0.58 -0.4 1,829,475 + +0.0 1,829,483 - +0.5 1,829,535 + +0.4 1,829,560 - -1.1 1,829,570 - +1.3 1,829,570 - -3.7 1,829,633 + +0.2 1,829,680 - CSW01_08540 0.14 +1.4 1,829,686 - CSW01_08540 0.15 -0.3 1,829,736 + CSW01_08540 0.21 -1.5 1,829,804 + CSW01_08540 0.29 -1.6 1,829,817 - CSW01_08540 0.31 +0.7 1,829,827 - CSW01_08540 0.32 -0.1
Or see this region's nucleotide sequence