Experiment: ICP1_phage_0.23_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_07165 and CSW01_07170 overlap by 11 nucleotides CSW01_07170 and CSW01_07175 are separated by 1 nucleotides CSW01_07175 and CSW01_07180 are separated by 76 nucleotides
CSW01_07165: CSW01_07165 - hypothetical protein, at 1,525,956 to 1,526,801
_07165
CSW01_07170: CSW01_07170 - glucosaminidase, at 1,526,791 to 1,527,594
_07170
CSW01_07175: CSW01_07175 - DUF2987 domain-containing protein, at 1,527,596 to 1,528,255
_07175
CSW01_07180: CSW01_07180 - tRNA 2-thiocytidine(32) synthetase TtcA, at 1,528,332 to 1,529,264
_07180
Position (kb) 1527
1528
1529 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1526.637 kb on + strand, within CSW01_07165 at 1526.647 kb on + strand, within CSW01_07165 at 1526.652 kb on + strand, within CSW01_07165 at 1526.658 kb on + strand, within CSW01_07165 at 1526.666 kb on - strand, within CSW01_07165 at 1526.666 kb on - strand, within CSW01_07165 at 1526.671 kb on - strand, within CSW01_07165 at 1526.755 kb on - strand at 1526.810 kb on + strand at 1526.903 kb on + strand, within CSW01_07170 at 1526.903 kb on + strand, within CSW01_07170 at 1526.903 kb on + strand, within CSW01_07170 at 1526.903 kb on + strand, within CSW01_07170 at 1526.903 kb on + strand, within CSW01_07170 at 1526.903 kb on + strand, within CSW01_07170 at 1526.911 kb on - strand, within CSW01_07170 at 1526.989 kb on - strand, within CSW01_07170 at 1527.201 kb on - strand, within CSW01_07170 at 1527.272 kb on + strand, within CSW01_07170 at 1527.305 kb on + strand, within CSW01_07170 at 1527.348 kb on + strand, within CSW01_07170 at 1527.503 kb on - strand, within CSW01_07170 at 1527.766 kb on + strand, within CSW01_07175 at 1527.824 kb on + strand, within CSW01_07175 at 1527.863 kb on + strand, within CSW01_07175 at 1528.020 kb on + strand, within CSW01_07175 at 1528.028 kb on - strand, within CSW01_07175 at 1528.127 kb on + strand, within CSW01_07175 at 1528.135 kb on - strand, within CSW01_07175 at 1528.327 kb on - strand at 1528.346 kb on + strand at 1528.528 kb on + strand, within CSW01_07180 at 1528.528 kb on + strand, within CSW01_07180 at 1528.536 kb on - strand, within CSW01_07180 at 1528.536 kb on - strand, within CSW01_07180 at 1528.586 kb on + strand, within CSW01_07180 at 1528.606 kb on + strand, within CSW01_07180 at 1528.613 kb on + strand, within CSW01_07180 at 1528.614 kb on - strand, within CSW01_07180 at 1528.860 kb on + strand, within CSW01_07180 at 1528.868 kb on - strand, within CSW01_07180 at 1528.888 kb on - strand, within CSW01_07180 at 1528.980 kb on + strand, within CSW01_07180 at 1529.006 kb on - strand, within CSW01_07180 at 1529.193 kb on - strand at 1529.243 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_0.23_MOI_rep2 remove 1,526,637 + CSW01_07165 0.80 +1.4 1,526,647 + CSW01_07165 0.82 +0.1 1,526,652 + CSW01_07165 0.82 -0.7 1,526,658 + CSW01_07165 0.83 -0.6 1,526,666 - CSW01_07165 0.84 -1.2 1,526,666 - CSW01_07165 0.84 -1.1 1,526,671 - CSW01_07165 0.85 -3.8 1,526,755 - -0.3 1,526,810 + -1.1 1,526,903 + CSW01_07170 0.14 +0.3 1,526,903 + CSW01_07170 0.14 -1.0 1,526,903 + CSW01_07170 0.14 -2.0 1,526,903 + CSW01_07170 0.14 +0.5 1,526,903 + CSW01_07170 0.14 +1.0 1,526,903 + CSW01_07170 0.14 -0.8 1,526,911 - CSW01_07170 0.15 -0.2 1,526,989 - CSW01_07170 0.25 +1.7 1,527,201 - CSW01_07170 0.51 +1.2 1,527,272 + CSW01_07170 0.60 +1.4 1,527,305 + CSW01_07170 0.64 +3.0 1,527,348 + CSW01_07170 0.69 -2.9 1,527,503 - CSW01_07170 0.89 -1.8 1,527,766 + CSW01_07175 0.26 -1.2 1,527,824 + CSW01_07175 0.35 -1.8 1,527,863 + CSW01_07175 0.40 -1.8 1,528,020 + CSW01_07175 0.64 -0.6 1,528,028 - CSW01_07175 0.65 -0.5 1,528,127 + CSW01_07175 0.80 -1.0 1,528,135 - CSW01_07175 0.82 -2.9 1,528,327 - -0.4 1,528,346 + +1.2 1,528,528 + CSW01_07180 0.21 -0.6 1,528,528 + CSW01_07180 0.21 -1.8 1,528,536 - CSW01_07180 0.22 +0.2 1,528,536 - CSW01_07180 0.22 +0.3 1,528,586 + CSW01_07180 0.27 +0.2 1,528,606 + CSW01_07180 0.29 +1.0 1,528,613 + CSW01_07180 0.30 +0.8 1,528,614 - CSW01_07180 0.30 -3.0 1,528,860 + CSW01_07180 0.57 +1.6 1,528,868 - CSW01_07180 0.57 +1.1 1,528,888 - CSW01_07180 0.60 +1.4 1,528,980 + CSW01_07180 0.69 -0.5 1,529,006 - CSW01_07180 0.72 +0.6 1,529,193 - +0.5 1,529,243 + -2.5
Or see this region's nucleotide sequence