Experiment: ICP1_phage_0.23_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_04920 and CSW01_04925 are separated by 112 nucleotides CSW01_04925 and CSW01_04930 are separated by 108 nucleotides CSW01_04930 and CSW01_04935 are separated by 133 nucleotides CSW01_04935 and CSW01_04940 are separated by 84 nucleotides CSW01_04940 and CSW01_04945 are separated by 3 nucleotides
CSW01_04920: CSW01_04920 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 1,026,576 to 1,027,769
_04920
CSW01_04925: CSW01_04925 - PTS glucose transporter subunit IIA, at 1,027,882 to 1,028,391
_04925
CSW01_04930: CSW01_04930 - phosphoenolpyruvate-protein phosphotransferase PtsI, at 1,028,500 to 1,030,221
_04930
CSW01_04935: CSW01_04935 - phosphocarrier protein HPr, at 1,030,355 to 1,030,612
_04935
CSW01_04940: CSW01_04940 - hypothetical protein, at 1,030,697 to 1,030,906
_04940
CSW01_04945: CSW01_04945 - cysteine synthase A, at 1,030,910 to 1,031,878
_04945
Position (kb)
1028
1029
1030
1031 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1027.742 kb on + strand at 1027.757 kb on - strand at 1027.833 kb on + strand at 1027.969 kb on - strand, within CSW01_04925 at 1028.138 kb on - strand, within CSW01_04925 at 1028.393 kb on + strand at 1028.401 kb on + strand at 1028.433 kb on + strand at 1028.518 kb on + strand at 1028.549 kb on + strand at 1028.842 kb on + strand, within CSW01_04930 at 1028.850 kb on - strand, within CSW01_04930 at 1028.907 kb on - strand, within CSW01_04930 at 1028.990 kb on + strand, within CSW01_04930 at 1028.998 kb on - strand, within CSW01_04930 at 1028.998 kb on - strand, within CSW01_04930 at 1029.075 kb on + strand, within CSW01_04930 at 1029.075 kb on + strand, within CSW01_04930 at 1029.075 kb on + strand, within CSW01_04930 at 1029.114 kb on + strand, within CSW01_04930 at 1029.126 kb on + strand, within CSW01_04930 at 1029.134 kb on - strand, within CSW01_04930 at 1029.134 kb on - strand, within CSW01_04930 at 1029.144 kb on + strand, within CSW01_04930 at 1029.237 kb on - strand, within CSW01_04930 at 1029.419 kb on - strand, within CSW01_04930 at 1029.627 kb on + strand, within CSW01_04930 at 1029.635 kb on - strand, within CSW01_04930 at 1029.635 kb on - strand, within CSW01_04930 at 1029.635 kb on - strand, within CSW01_04930 at 1029.635 kb on - strand, within CSW01_04930 at 1029.635 kb on - strand, within CSW01_04930 at 1029.635 kb on - strand, within CSW01_04930 at 1029.635 kb on - strand, within CSW01_04930 at 1029.704 kb on + strand, within CSW01_04930 at 1030.088 kb on + strand at 1030.096 kb on - strand at 1030.096 kb on - strand at 1030.096 kb on - strand at 1030.170 kb on + strand at 1030.228 kb on + strand at 1030.236 kb on - strand at 1030.281 kb on - strand at 1030.309 kb on - strand at 1030.440 kb on + strand, within CSW01_04935 at 1030.450 kb on + strand, within CSW01_04935 at 1030.725 kb on - strand, within CSW01_04940 at 1030.778 kb on + strand, within CSW01_04940 at 1031.061 kb on + strand, within CSW01_04945 at 1031.084 kb on - strand, within CSW01_04945 at 1031.217 kb on + strand, within CSW01_04945
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_0.23_MOI_rep2 remove 1,027,742 + +0.6 1,027,757 - -0.2 1,027,833 + +1.0 1,027,969 - CSW01_04925 0.17 +1.2 1,028,138 - CSW01_04925 0.50 +0.6 1,028,393 + +2.8 1,028,401 + +3.0 1,028,433 + -1.2 1,028,518 + +0.1 1,028,549 + -0.7 1,028,842 + CSW01_04930 0.20 -1.4 1,028,850 - CSW01_04930 0.20 -1.7 1,028,907 - CSW01_04930 0.24 -1.4 1,028,990 + CSW01_04930 0.28 -2.0 1,028,998 - CSW01_04930 0.29 -0.9 1,028,998 - CSW01_04930 0.29 -0.3 1,029,075 + CSW01_04930 0.33 -0.2 1,029,075 + CSW01_04930 0.33 +0.9 1,029,075 + CSW01_04930 0.33 -1.0 1,029,114 + CSW01_04930 0.36 -0.1 1,029,126 + CSW01_04930 0.36 -2.2 1,029,134 - CSW01_04930 0.37 -0.9 1,029,134 - CSW01_04930 0.37 -2.5 1,029,144 + CSW01_04930 0.37 +0.7 1,029,237 - CSW01_04930 0.43 -0.9 1,029,419 - CSW01_04930 0.53 -0.4 1,029,627 + CSW01_04930 0.65 -0.6 1,029,635 - CSW01_04930 0.66 -2.7 1,029,635 - CSW01_04930 0.66 -1.1 1,029,635 - CSW01_04930 0.66 -0.4 1,029,635 - CSW01_04930 0.66 +0.5 1,029,635 - CSW01_04930 0.66 -1.2 1,029,635 - CSW01_04930 0.66 -0.3 1,029,635 - CSW01_04930 0.66 -1.1 1,029,704 + CSW01_04930 0.70 -1.8 1,030,088 + +0.3 1,030,096 - -1.7 1,030,096 - +1.2 1,030,096 - +1.6 1,030,170 + +0.4 1,030,228 + +1.3 1,030,236 - -2.0 1,030,281 - +0.9 1,030,309 - +1.5 1,030,440 + CSW01_04935 0.33 +2.5 1,030,450 + CSW01_04935 0.37 +3.1 1,030,725 - CSW01_04940 0.13 -1.7 1,030,778 + CSW01_04940 0.39 -3.9 1,031,061 + CSW01_04945 0.16 +1.1 1,031,084 - CSW01_04945 0.18 -2.3 1,031,217 + CSW01_04945 0.32 +0.2
Or see this region's nucleotide sequence