Experiment: Choline Lysine 12 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_25305 and AO353_25310 are separated by 1 nucleotides AO353_25310 and AO353_25315 are separated by 64 nucleotides AO353_25315 and AO353_25320 overlap by 4 nucleotides AO353_25320 and AO353_25325 overlap by 7 nucleotides
AO353_25305: AO353_25305 - malonate transporter, at 5,583,257 to 5,584,021
_25305
AO353_25310: AO353_25310 - malonate carrier protein, at 5,584,023 to 5,584,439
_25310
AO353_25315: AO353_25315 - malonate decarboxylase subunit epsilon, at 5,584,504 to 5,585,424
_25315
AO353_25320: AO353_25320 - phosphoribosyl-dephospho-CoA transferase, at 5,585,421 to 5,586,041
_25320
AO353_25325: AO353_25325 - biotin-independent malonate decarboxylase subunit gamma, at 5,586,035 to 5,586,838
_25325
Position (kb) 5584
5585
5586 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5583.579 kb on + strand, within AO353_25305 at 5583.579 kb on + strand, within AO353_25305 at 5583.579 kb on + strand, within AO353_25305 at 5583.580 kb on - strand, within AO353_25305 at 5583.580 kb on - strand, within AO353_25305 at 5583.580 kb on - strand, within AO353_25305 at 5583.580 kb on - strand, within AO353_25305 at 5583.580 kb on - strand, within AO353_25305 at 5583.580 kb on - strand, within AO353_25305 at 5583.662 kb on - strand, within AO353_25305 at 5583.662 kb on - strand, within AO353_25305 at 5583.662 kb on - strand, within AO353_25305 at 5584.021 kb on + strand at 5584.021 kb on + strand at 5584.022 kb on - strand at 5584.022 kb on - strand at 5584.022 kb on - strand at 5584.022 kb on - strand at 5584.228 kb on + strand, within AO353_25310 at 5584.228 kb on + strand, within AO353_25310 at 5584.229 kb on - strand, within AO353_25310 at 5584.229 kb on - strand, within AO353_25310 at 5584.229 kb on - strand, within AO353_25310 at 5584.229 kb on - strand, within AO353_25310 at 5584.229 kb on - strand, within AO353_25310 at 5584.429 kb on + strand at 5584.429 kb on + strand at 5584.434 kb on - strand at 5584.434 kb on - strand at 5584.434 kb on - strand at 5584.434 kb on - strand at 5584.471 kb on + strand at 5584.471 kb on + strand at 5584.472 kb on - strand at 5584.675 kb on + strand, within AO353_25315 at 5584.826 kb on - strand, within AO353_25315 at 5585.199 kb on - strand, within AO353_25315 at 5585.208 kb on + strand, within AO353_25315 at 5585.208 kb on + strand, within AO353_25315 at 5585.208 kb on + strand, within AO353_25315 at 5585.208 kb on + strand, within AO353_25315 at 5585.208 kb on + strand, within AO353_25315 at 5585.209 kb on - strand, within AO353_25315 at 5585.209 kb on - strand, within AO353_25315 at 5585.209 kb on - strand, within AO353_25315 at 5585.209 kb on - strand, within AO353_25315 at 5585.209 kb on - strand, within AO353_25315 at 5585.308 kb on + strand, within AO353_25315 at 5585.858 kb on - strand, within AO353_25320 at 5585.858 kb on - strand, within AO353_25320 at 5585.858 kb on - strand, within AO353_25320 at 5585.883 kb on + strand, within AO353_25320 at 5585.883 kb on + strand, within AO353_25320 at 5585.883 kb on + strand, within AO353_25320 at 5585.884 kb on - strand, within AO353_25320 at 5585.884 kb on - strand, within AO353_25320 at 5586.150 kb on - strand, within AO353_25325 at 5586.240 kb on + strand, within AO353_25325 at 5586.240 kb on + strand, within AO353_25325 at 5586.240 kb on + strand, within AO353_25325 at 5586.240 kb on + strand, within AO353_25325 at 5586.241 kb on - strand, within AO353_25325 at 5586.241 kb on - strand, within AO353_25325 at 5586.241 kb on - strand, within AO353_25325 at 5586.241 kb on - strand, within AO353_25325 at 5586.241 kb on - strand, within AO353_25325 at 5586.241 kb on - strand, within AO353_25325 at 5586.241 kb on - strand, within AO353_25325 at 5586.241 kb on - strand at 5586.255 kb on + strand, within AO353_25325 at 5586.256 kb on - strand, within AO353_25325
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 12 mM remove 5,583,579 + AO353_25305 0.42 +1.9 5,583,579 + AO353_25305 0.42 -0.5 5,583,579 + AO353_25305 0.42 +1.4 5,583,580 - AO353_25305 0.42 +0.8 5,583,580 - AO353_25305 0.42 +1.4 5,583,580 - AO353_25305 0.42 -1.3 5,583,580 - AO353_25305 0.42 +0.1 5,583,580 - AO353_25305 0.42 +0.8 5,583,580 - AO353_25305 0.42 +1.0 5,583,662 - AO353_25305 0.53 -0.1 5,583,662 - AO353_25305 0.53 +0.7 5,583,662 - AO353_25305 0.53 +0.9 5,584,021 + -0.7 5,584,021 + +0.3 5,584,022 - +0.1 5,584,022 - +0.9 5,584,022 - -0.3 5,584,022 - +1.0 5,584,228 + AO353_25310 0.49 +0.5 5,584,228 + AO353_25310 0.49 -1.7 5,584,229 - AO353_25310 0.49 -0.7 5,584,229 - AO353_25310 0.49 +0.1 5,584,229 - AO353_25310 0.49 -0.1 5,584,229 - AO353_25310 0.49 -2.1 5,584,229 - AO353_25310 0.49 -1.9 5,584,429 + -2.1 5,584,429 + -0.7 5,584,434 - +1.3 5,584,434 - +0.9 5,584,434 - -1.5 5,584,434 - -0.5 5,584,471 + +1.0 5,584,471 + -0.7 5,584,472 - +0.3 5,584,675 + AO353_25315 0.19 +0.3 5,584,826 - AO353_25315 0.35 +0.3 5,585,199 - AO353_25315 0.75 -1.3 5,585,208 + AO353_25315 0.76 -1.1 5,585,208 + AO353_25315 0.76 -1.3 5,585,208 + AO353_25315 0.76 -1.3 5,585,208 + AO353_25315 0.76 -2.2 5,585,208 + AO353_25315 0.76 -1.0 5,585,209 - AO353_25315 0.77 +1.3 5,585,209 - AO353_25315 0.77 +0.1 5,585,209 - AO353_25315 0.77 -1.7 5,585,209 - AO353_25315 0.77 +2.3 5,585,209 - AO353_25315 0.77 +0.3 5,585,308 + AO353_25315 0.87 -2.3 5,585,858 - AO353_25320 0.70 -1.0 5,585,858 - AO353_25320 0.70 +0.9 5,585,858 - AO353_25320 0.70 +1.0 5,585,883 + AO353_25320 0.74 +1.0 5,585,883 + AO353_25320 0.74 -1.3 5,585,883 + AO353_25320 0.74 +0.3 5,585,884 - AO353_25320 0.75 -0.3 5,585,884 - AO353_25320 0.75 -0.1 5,586,150 - AO353_25325 0.14 +1.0 5,586,240 + AO353_25325 0.25 -0.9 5,586,240 + AO353_25325 0.25 +1.7 5,586,240 + AO353_25325 0.25 -0.0 5,586,240 + AO353_25325 0.25 -0.1 5,586,241 - AO353_25325 0.26 +0.5 5,586,241 - AO353_25325 0.26 +1.6 5,586,241 - AO353_25325 0.26 +1.9 5,586,241 - AO353_25325 0.26 -0.0 5,586,241 - AO353_25325 0.26 +0.9 5,586,241 - AO353_25325 0.26 +0.7 5,586,241 - AO353_25325 0.26 +1.0 5,586,241 - +0.3 5,586,255 + AO353_25325 0.27 -0.1 5,586,256 - AO353_25325 0.27 +1.3
Or see this region's nucleotide sequence