Experiment: Choline Lysine 12 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_02025 and AO353_02030 are separated by 831 nucleotides AO353_02030 and AO353_02035 overlap by 1 nucleotides AO353_02035 and AO353_02040 are separated by 24 nucleotides AO353_02040 and AO353_02045 are separated by 174 nucleotides
AO353_02025: AO353_02025 - mannose-1-phosphate guanyltransferase, at 431,568 to 433,028
_02025
AO353_02030: AO353_02030 - hypothetical protein, at 433,860 to 434,039
_02030
AO353_02035: AO353_02035 - hypothetical protein, at 434,039 to 434,278
_02035
AO353_02040: AO353_02040 - integration host factor subunit beta, at 434,303 to 434,599
_02040
AO353_02045: AO353_02045 - hypothetical protein, at 434,774 to 435,055
_02045
Position (kb) 433
434
435 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 432.864 kb on - strand, within AO353_02025 at 432.873 kb on + strand, within AO353_02025 at 432.873 kb on + strand, within AO353_02025 at 432.873 kb on + strand, within AO353_02025 at 432.873 kb on + strand, within AO353_02025 at 432.874 kb on - strand, within AO353_02025 at 432.874 kb on - strand, within AO353_02025 at 432.918 kb on + strand at 432.918 kb on + strand at 432.919 kb on - strand at 433.042 kb on - strand at 433.064 kb on - strand at 433.067 kb on + strand at 433.067 kb on + strand at 433.067 kb on + strand at 433.067 kb on + strand at 433.068 kb on - strand at 433.068 kb on - strand at 433.068 kb on - strand at 433.087 kb on + strand at 433.087 kb on + strand at 433.087 kb on + strand at 433.087 kb on + strand at 433.087 kb on + strand at 433.087 kb on + strand at 433.088 kb on - strand at 433.088 kb on - strand at 433.089 kb on + strand at 433.089 kb on + strand at 433.089 kb on + strand at 433.090 kb on - strand at 433.172 kb on - strand at 433.189 kb on - strand at 433.198 kb on + strand at 433.198 kb on + strand at 433.198 kb on + strand at 433.199 kb on - strand at 433.199 kb on - strand at 433.216 kb on + strand at 433.217 kb on - strand at 433.217 kb on - strand at 433.217 kb on - strand at 433.255 kb on + strand at 433.255 kb on + strand at 433.255 kb on + strand at 433.256 kb on - strand at 433.261 kb on + strand at 433.261 kb on + strand at 433.261 kb on + strand at 433.360 kb on + strand at 433.360 kb on + strand at 433.360 kb on + strand at 433.451 kb on + strand at 433.451 kb on + strand at 433.510 kb on + strand at 433.511 kb on - strand at 433.555 kb on + strand at 433.555 kb on + strand at 433.555 kb on + strand at 433.556 kb on - strand at 433.556 kb on - strand at 433.556 kb on - strand at 433.565 kb on - strand at 433.565 kb on - strand at 433.565 kb on - strand at 433.565 kb on - strand at 433.565 kb on - strand at 433.565 kb on - strand at 433.628 kb on + strand at 433.628 kb on + strand at 433.629 kb on - strand at 433.629 kb on - strand at 433.629 kb on - strand at 433.629 kb on - strand at 433.630 kb on + strand at 433.630 kb on + strand at 433.630 kb on + strand at 433.631 kb on - strand at 433.737 kb on + strand at 433.738 kb on - strand at 433.738 kb on - strand at 433.752 kb on + strand at 433.766 kb on + strand at 433.853 kb on + strand at 433.853 kb on + strand at 433.854 kb on - strand at 433.858 kb on + strand at 433.859 kb on - strand at 433.883 kb on + strand, within AO353_02030 at 433.883 kb on + strand, within AO353_02030 at 433.883 kb on + strand, within AO353_02030 at 433.883 kb on + strand, within AO353_02030 at 433.884 kb on - strand, within AO353_02030 at 433.884 kb on - strand, within AO353_02030 at 433.884 kb on - strand, within AO353_02030 at 433.884 kb on - strand, within AO353_02030 at 433.890 kb on - strand, within AO353_02030 at 433.890 kb on - strand, within AO353_02030 at 433.961 kb on + strand, within AO353_02030 at 434.005 kb on + strand, within AO353_02030 at 434.020 kb on + strand, within AO353_02030 at 434.021 kb on - strand, within AO353_02030 at 434.053 kb on + strand at 434.053 kb on + strand at 434.054 kb on - strand at 434.258 kb on + strand at 434.258 kb on + strand at 434.259 kb on - strand at 434.272 kb on + strand at 434.272 kb on + strand at 434.273 kb on - strand at 434.273 kb on - strand at 434.273 kb on - strand at 434.378 kb on - strand, within AO353_02040 at 434.414 kb on - strand, within AO353_02040 at 434.436 kb on + strand, within AO353_02040 at 434.441 kb on + strand, within AO353_02040 at 434.553 kb on - strand, within AO353_02040 at 434.553 kb on - strand, within AO353_02040 at 434.600 kb on - strand at 434.625 kb on + strand at 434.625 kb on + strand at 434.648 kb on - strand at 434.648 kb on - strand at 434.675 kb on + strand at 434.675 kb on + strand at 434.675 kb on + strand at 434.676 kb on - strand at 434.676 kb on - strand at 434.677 kb on + strand at 434.677 kb on + strand at 434.677 kb on + strand at 434.677 kb on + strand at 434.677 kb on + strand at 434.677 kb on + strand at 434.677 kb on + strand at 434.678 kb on - strand at 434.678 kb on - strand at 434.678 kb on - strand at 434.678 kb on - strand at 434.678 kb on - strand at 434.678 kb on - strand at 434.679 kb on + strand at 434.679 kb on + strand at 434.679 kb on + strand at 434.679 kb on + strand at 434.679 kb on + strand at 434.679 kb on + strand at 434.680 kb on - strand at 434.680 kb on - strand at 434.680 kb on - strand at 434.680 kb on - strand at 434.680 kb on - strand at 434.680 kb on - strand at 434.680 kb on - strand at 434.680 kb on - strand at 434.687 kb on + strand at 434.687 kb on + strand at 434.693 kb on + strand at 434.693 kb on + strand at 434.693 kb on + strand at 434.693 kb on + strand at 434.831 kb on + strand, within AO353_02045 at 434.831 kb on + strand, within AO353_02045 at 434.832 kb on - strand, within AO353_02045 at 434.832 kb on - strand, within AO353_02045 at 434.832 kb on - strand, within AO353_02045 at 434.832 kb on - strand, within AO353_02045 at 434.835 kb on + strand, within AO353_02045 at 434.835 kb on + strand, within AO353_02045 at 434.835 kb on + strand, within AO353_02045 at 434.835 kb on + strand, within AO353_02045 at 434.835 kb on + strand, within AO353_02045 at 434.836 kb on - strand, within AO353_02045 at 434.836 kb on - strand, within AO353_02045 at 434.836 kb on - strand, within AO353_02045 at 434.836 kb on - strand, within AO353_02045 at 434.854 kb on + strand, within AO353_02045 at 434.854 kb on + strand, within AO353_02045 at 434.855 kb on - strand, within AO353_02045 at 434.883 kb on + strand, within AO353_02045 at 434.883 kb on + strand, within AO353_02045 at 434.919 kb on + strand, within AO353_02045 at 434.919 kb on + strand, within AO353_02045 at 434.919 kb on + strand, within AO353_02045 at 434.919 kb on + strand, within AO353_02045 at 434.920 kb on - strand, within AO353_02045 at 434.920 kb on - strand, within AO353_02045 at 434.920 kb on - strand, within AO353_02045 at 434.926 kb on + strand, within AO353_02045 at 434.927 kb on - strand, within AO353_02045 at 434.927 kb on - strand, within AO353_02045 at 435.007 kb on + strand, within AO353_02045 at 435.007 kb on + strand, within AO353_02045 at 435.007 kb on + strand, within AO353_02045 at 435.007 kb on + strand, within AO353_02045 at 435.007 kb on + strand, within AO353_02045 at 435.008 kb on - strand, within AO353_02045 at 435.008 kb on - strand, within AO353_02045 at 435.008 kb on - strand, within AO353_02045
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 12 mM remove 432,864 - AO353_02025 0.89 -0.6 432,873 + AO353_02025 0.89 -1.3 432,873 + AO353_02025 0.89 +0.7 432,873 + AO353_02025 0.89 +0.7 432,873 + AO353_02025 0.89 -0.8 432,874 - AO353_02025 0.89 +0.7 432,874 - AO353_02025 0.89 -0.8 432,918 + -2.4 432,918 + -0.3 432,919 - -0.3 433,042 - +1.2 433,064 - +2.2 433,067 + +1.5 433,067 + -0.8 433,067 + -1.0 433,067 + +0.2 433,068 - +1.4 433,068 - -0.5 433,068 - +0.7 433,087 + +1.3 433,087 + -2.2 433,087 + +0.5 433,087 + -0.7 433,087 + -1.6 433,087 + +1.2 433,088 - +0.2 433,088 - -0.3 433,089 + -1.8 433,089 + -0.1 433,089 + -0.8 433,090 - -1.8 433,172 - -0.3 433,189 - +1.1 433,198 + -0.3 433,198 + -0.8 433,198 + +0.7 433,199 - +0.0 433,199 - -2.1 433,216 + +0.7 433,217 - -0.8 433,217 - -0.9 433,217 - -0.3 433,255 + +0.6 433,255 + -0.7 433,255 + -1.3 433,256 - -0.3 433,261 + -0.6 433,261 + -0.3 433,261 + -0.0 433,360 + -0.3 433,360 + +0.2 433,360 + +3.3 433,451 + +0.2 433,451 + +1.2 433,510 + -0.5 433,511 - +1.3 433,555 + +0.1 433,555 + +0.3 433,555 + -1.8 433,556 - +1.6 433,556 - +0.3 433,556 - +0.4 433,565 - -0.6 433,565 - +0.5 433,565 - -2.4 433,565 - +0.2 433,565 - +0.3 433,565 - -0.8 433,628 + -0.3 433,628 + +0.3 433,629 - +0.2 433,629 - +0.7 433,629 - +0.2 433,629 - -0.3 433,630 + -0.8 433,630 + +0.3 433,630 + -1.0 433,631 - +2.0 433,737 + +0.7 433,738 - -0.6 433,738 - +0.5 433,752 + +2.7 433,766 + +0.2 433,853 + +1.2 433,853 + +0.9 433,854 - +1.7 433,858 + +2.3 433,859 - -0.3 433,883 + AO353_02030 0.13 +0.7 433,883 + AO353_02030 0.13 -0.6 433,883 + AO353_02030 0.13 +0.3 433,883 + AO353_02030 0.13 +0.2 433,884 - AO353_02030 0.13 -0.3 433,884 - AO353_02030 0.13 +1.7 433,884 - AO353_02030 0.13 +0.3 433,884 - AO353_02030 0.13 -0.0 433,890 - AO353_02030 0.17 +1.5 433,890 - AO353_02030 0.17 -0.3 433,961 + AO353_02030 0.56 -0.8 434,005 + AO353_02030 0.81 +1.5 434,020 + AO353_02030 0.89 +0.4 434,021 - AO353_02030 0.89 +2.0 434,053 + +0.4 434,053 + +0.2 434,054 - -0.3 434,258 + -0.8 434,258 + -1.6 434,259 - +1.3 434,272 + -1.6 434,272 + +0.5 434,273 - +0.7 434,273 - +0.3 434,273 - +0.7 434,378 - AO353_02040 0.25 +1.0 434,414 - AO353_02040 0.37 +0.9 434,436 + AO353_02040 0.45 -1.8 434,441 + AO353_02040 0.46 -0.3 434,553 - AO353_02040 0.84 -0.6 434,553 - AO353_02040 0.84 -1.4 434,600 - +1.0 434,625 + -1.3 434,625 + -0.0 434,648 - -0.7 434,648 - -0.3 434,675 + +1.5 434,675 + +1.3 434,675 + +0.3 434,676 - -0.6 434,676 - -0.1 434,677 + +0.9 434,677 + -0.3 434,677 + +1.3 434,677 + -0.1 434,677 + +1.3 434,677 + -1.3 434,677 + -0.7 434,678 - -0.3 434,678 - +0.4 434,678 - +0.7 434,678 - -0.3 434,678 - +0.6 434,678 - +0.3 434,679 + -0.5 434,679 + +0.2 434,679 + +0.2 434,679 + -1.6 434,679 + +0.2 434,679 + -0.0 434,680 - +0.2 434,680 - -2.1 434,680 - +0.0 434,680 - +0.3 434,680 - -0.7 434,680 - +0.7 434,680 - +1.3 434,680 - +2.3 434,687 + -0.5 434,687 + +0.6 434,693 + -0.8 434,693 + -0.3 434,693 + -0.3 434,693 + +2.4 434,831 + AO353_02045 0.20 +0.4 434,831 + AO353_02045 0.20 +1.6 434,832 - AO353_02045 0.21 +1.2 434,832 - AO353_02045 0.21 -0.8 434,832 - AO353_02045 0.21 -1.3 434,832 - AO353_02045 0.21 +0.3 434,835 + AO353_02045 0.22 -0.8 434,835 + AO353_02045 0.22 +0.3 434,835 + AO353_02045 0.22 +0.3 434,835 + AO353_02045 0.22 -0.3 434,835 + AO353_02045 0.22 +1.1 434,836 - AO353_02045 0.22 -1.1 434,836 - AO353_02045 0.22 -0.9 434,836 - AO353_02045 0.22 +0.6 434,836 - AO353_02045 0.22 -0.0 434,854 + AO353_02045 0.28 +0.3 434,854 + AO353_02045 0.28 +1.1 434,855 - AO353_02045 0.29 +1.3 434,883 + AO353_02045 0.39 +0.5 434,883 + AO353_02045 0.39 +0.9 434,919 + AO353_02045 0.51 +1.2 434,919 + AO353_02045 0.51 +0.2 434,919 + AO353_02045 0.51 -0.8 434,919 + AO353_02045 0.51 +1.0 434,920 - AO353_02045 0.52 +1.6 434,920 - AO353_02045 0.52 +0.2 434,920 - AO353_02045 0.52 +0.2 434,926 + AO353_02045 0.54 +0.7 434,927 - AO353_02045 0.54 -1.7 434,927 - AO353_02045 0.54 -0.2 435,007 + AO353_02045 0.83 -0.8 435,007 + AO353_02045 0.83 +0.7 435,007 + AO353_02045 0.83 -1.6 435,007 + AO353_02045 0.83 -0.4 435,007 + AO353_02045 0.83 -0.1 435,008 - AO353_02045 0.83 +0.3 435,008 - AO353_02045 0.83 +0.4 435,008 - AO353_02045 0.83 -1.6
Or see this region's nucleotide sequence