Experiment: MoYLS4 with HQNO 100uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheA-3 and cheY-2 are separated by 95 nucleotides cheY-2 and DVU2074 are separated by 9 nucleotides DVU2074 and parA overlap by 7 nucleotides parA and cheR-2 are separated by 50 nucleotides
DVU2072: cheA-3 - chemotaxis protein CheA (TIGR), at 2,154,133 to 2,157,402
cheA-3
DVU2073: cheY-2 - chemotaxis protein CheY (TIGR), at 2,157,498 to 2,157,866
cheY-2
DVU2074: DVU2074 - chemotaxis protein CheW (TIGR), at 2,157,876 to 2,158,526
DVU2074
DVU2075: parA - ParA family protein (TIGR), at 2,158,520 to 2,159,296
parA
DVU2076: cheR-2 - chemotaxis protein methyltransferase (TIGR), at 2,159,347 to 2,160,225
cheR-2
Position (kb)
2157
2158
2159 Strain fitness (log2 ratio)
-2
-1
0
1 at 2157.125 kb on - strand at 2157.281 kb on + strand at 2157.289 kb on + strand at 2157.297 kb on - strand at 2157.300 kb on - strand at 2157.386 kb on + strand at 2157.410 kb on + strand at 2157.410 kb on + strand at 2157.418 kb on - strand at 2157.418 kb on - strand at 2157.428 kb on - strand at 2157.458 kb on + strand at 2157.458 kb on + strand at 2157.458 kb on + strand at 2157.458 kb on + strand at 2157.458 kb on + strand at 2157.466 kb on - strand at 2157.555 kb on - strand, within cheY-2 at 2157.584 kb on - strand, within cheY-2 at 2157.664 kb on + strand, within cheY-2 at 2157.664 kb on + strand, within cheY-2 at 2157.672 kb on - strand, within cheY-2 at 2157.682 kb on - strand, within cheY-2 at 2157.682 kb on - strand, within cheY-2 at 2157.775 kb on - strand, within cheY-2 at 2157.782 kb on + strand, within cheY-2 at 2157.857 kb on - strand at 2157.948 kb on - strand, within DVU2074 at 2158.029 kb on - strand, within DVU2074 at 2158.099 kb on + strand, within DVU2074 at 2158.120 kb on + strand, within DVU2074 at 2158.208 kb on + strand, within DVU2074 at 2158.767 kb on + strand, within parA at 2158.847 kb on - strand, within parA at 2159.074 kb on - strand, within parA at 2159.221 kb on + strand at 2159.228 kb on + strand at 2159.292 kb on - strand at 2159.292 kb on - strand at 2159.292 kb on - strand at 2159.345 kb on + strand at 2159.345 kb on + strand at 2159.345 kb on + strand at 2159.345 kb on + strand at 2159.353 kb on - strand at 2159.358 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with HQNO 100uM remove 2,157,125 - -1.2 2,157,281 + -0.9 2,157,289 + -1.0 2,157,297 - -1.0 2,157,300 - -1.6 2,157,386 + -2.2 2,157,410 + -2.3 2,157,410 + -1.0 2,157,418 - -1.0 2,157,418 - -1.3 2,157,428 - +0.4 2,157,458 + -0.6 2,157,458 + -0.7 2,157,458 + -0.4 2,157,458 + -0.9 2,157,458 + -1.2 2,157,466 - -1.0 2,157,555 - cheY-2 DVU2073 0.15 -1.2 2,157,584 - cheY-2 DVU2073 0.23 +0.9 2,157,664 + cheY-2 DVU2073 0.45 -1.3 2,157,664 + cheY-2 DVU2073 0.45 -1.8 2,157,672 - cheY-2 DVU2073 0.47 -1.4 2,157,682 - cheY-2 DVU2073 0.50 -2.0 2,157,682 - cheY-2 DVU2073 0.50 -1.2 2,157,775 - cheY-2 DVU2073 0.75 -2.4 2,157,782 + cheY-2 DVU2073 0.77 -1.2 2,157,857 - -2.1 2,157,948 - DVU2074 0.11 -0.3 2,158,029 - DVU2074 0.24 -0.4 2,158,099 + DVU2074 0.34 -0.6 2,158,120 + DVU2074 0.37 -1.1 2,158,208 + DVU2074 0.51 -0.4 2,158,767 + parA DVU2075 0.32 -0.6 2,158,847 - parA DVU2075 0.42 -0.7 2,159,074 - parA DVU2075 0.71 +0.4 2,159,221 + -0.6 2,159,228 + +0.6 2,159,292 - +0.8 2,159,292 - -0.5 2,159,292 - -0.2 2,159,345 + +0.1 2,159,345 + +1.3 2,159,345 + +0.2 2,159,345 + -0.7 2,159,353 - -0.6 2,159,358 - +0.3
Or see this region's nucleotide sequence