Experiment: MoYLS4 with HQNO 100uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dxs and DVU1351 are separated by 242 nucleotides DVU1351 and DVU1352 are separated by 129 nucleotides DVU1352 and dnaE are separated by 1 nucleotides
DVU1350: dxs - deoxyxylulose-5-phosphate synthase (TIGR), at 1,427,612 to 1,429,537
dxs
DVU1351: DVU1351 - membrane protein, MarC family (TIGR), at 1,429,780 to 1,430,382
DVU1351
DVU1352: DVU1352 - 6-pyruvoyl tetrahydrobiopterin synthase, putative (TIGR), at 1,430,512 to 1,430,901
DVU1352
DVU1353: dnaE - DNA polymerase III, alpha subunit (TIGR), at 1,430,903 to 1,434,397
dnaE
Position (kb)
1429
1430
1431 Strain fitness (log2 ratio)
-2
-1
0
1 at 1429.511 kb on + strand at 1429.590 kb on + strand at 1429.610 kb on - strand at 1429.610 kb on - strand at 1429.623 kb on - strand at 1429.654 kb on + strand at 1429.654 kb on + strand at 1429.662 kb on + strand at 1429.662 kb on - strand at 1429.662 kb on - strand at 1429.669 kb on + strand at 1429.705 kb on + strand at 1429.779 kb on + strand at 1429.842 kb on + strand, within DVU1351 at 1429.902 kb on + strand, within DVU1351 at 1429.982 kb on - strand, within DVU1351 at 1429.998 kb on + strand, within DVU1351 at 1430.020 kb on + strand, within DVU1351 at 1430.379 kb on + strand at 1430.419 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with HQNO 100uM remove 1,429,511 + -0.9 1,429,590 + +0.4 1,429,610 - +0.4 1,429,610 - -0.1 1,429,623 - -0.3 1,429,654 + +0.1 1,429,654 + -0.4 1,429,662 + -0.0 1,429,662 - +0.5 1,429,662 - -0.2 1,429,669 + -0.3 1,429,705 + -0.8 1,429,779 + -1.3 1,429,842 + DVU1351 0.10 -0.5 1,429,902 + DVU1351 0.20 -0.8 1,429,982 - DVU1351 0.33 +0.6 1,429,998 + DVU1351 0.36 -1.9 1,430,020 + DVU1351 0.40 -0.5 1,430,379 + -0.8 1,430,419 - -0.2
Or see this region's nucleotide sequence