Experiment: D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_2464 and Synpcc7942_2465 are separated by 168 nucleotides Synpcc7942_2465 and Synpcc7942_2466 are separated by 263 nucleotides Synpcc7942_2466 and aroE are separated by 19 nucleotides
Synpcc7942_2464: Synpcc7942_2464 - N-acetylmannosamine-6-phosphate 2-epimerase, at 2,545,468 to 2,546,166
_2464
Synpcc7942_2465: Synpcc7942_2465 - hypothetical protein, at 2,546,335 to 2,546,586
_2465
Synpcc7942_2466: Synpcc7942_2466 - two component transcriptional regulator, winged helix family, at 2,546,850 to 2,547,557
_2466
Synpcc7942_2467: aroE - shikimate 5-dehydrogenase, at 2,547,577 to 2,548,443
aroE
Position (kb)
2546
2547
2548 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2545.853 kb on + strand, within Synpcc7942_2464 at 2545.866 kb on - strand, within Synpcc7942_2464 at 2545.907 kb on + strand, within Synpcc7942_2464 at 2545.924 kb on - strand, within Synpcc7942_2464 at 2545.925 kb on - strand, within Synpcc7942_2464 at 2545.925 kb on - strand, within Synpcc7942_2464 at 2545.936 kb on + strand, within Synpcc7942_2464 at 2545.944 kb on - strand, within Synpcc7942_2464 at 2545.999 kb on + strand, within Synpcc7942_2464 at 2546.007 kb on + strand, within Synpcc7942_2464 at 2546.010 kb on - strand, within Synpcc7942_2464 at 2546.015 kb on - strand, within Synpcc7942_2464 at 2546.026 kb on + strand, within Synpcc7942_2464 at 2546.044 kb on - strand, within Synpcc7942_2464 at 2546.049 kb on - strand, within Synpcc7942_2464 at 2546.054 kb on - strand, within Synpcc7942_2464 at 2546.054 kb on - strand, within Synpcc7942_2464 at 2546.076 kb on + strand, within Synpcc7942_2464 at 2546.081 kb on + strand, within Synpcc7942_2464 at 2546.130 kb on - strand at 2546.132 kb on + strand at 2546.139 kb on - strand at 2546.180 kb on - strand at 2546.181 kb on - strand at 2546.189 kb on + strand at 2546.197 kb on - strand at 2546.230 kb on + strand at 2546.231 kb on + strand at 2546.239 kb on - strand at 2546.255 kb on + strand at 2546.361 kb on + strand, within Synpcc7942_2465 at 2546.366 kb on + strand, within Synpcc7942_2465 at 2546.373 kb on + strand, within Synpcc7942_2465 at 2546.423 kb on - strand, within Synpcc7942_2465 at 2546.483 kb on + strand, within Synpcc7942_2465 at 2546.547 kb on + strand, within Synpcc7942_2465 at 2546.547 kb on + strand, within Synpcc7942_2465 at 2546.591 kb on - strand at 2546.615 kb on + strand at 2546.623 kb on - strand at 2546.623 kb on - strand at 2546.638 kb on + strand at 2546.649 kb on + strand at 2546.659 kb on + strand at 2546.667 kb on - strand at 2546.693 kb on + strand at 2546.693 kb on + strand at 2546.693 kb on + strand at 2546.701 kb on - strand at 2546.725 kb on - strand at 2546.751 kb on + strand at 2546.783 kb on - strand at 2546.783 kb on - strand at 2546.790 kb on - strand at 2546.794 kb on + strand at 2546.798 kb on + strand at 2546.824 kb on - strand at 2546.829 kb on + strand at 2546.830 kb on - strand at 2546.831 kb on + strand at 2546.837 kb on - strand at 2546.837 kb on - strand at 2546.837 kb on - strand at 2546.842 kb on + strand at 2546.842 kb on + strand at 2546.842 kb on + strand at 2546.864 kb on + strand at 2546.864 kb on + strand at 2546.864 kb on + strand at 2546.865 kb on + strand at 2546.892 kb on - strand at 2546.906 kb on + strand at 2546.909 kb on - strand at 2546.954 kb on + strand, within Synpcc7942_2466 at 2546.954 kb on + strand, within Synpcc7942_2466 at 2546.989 kb on + strand, within Synpcc7942_2466 at 2546.989 kb on + strand, within Synpcc7942_2466 at 2546.989 kb on + strand, within Synpcc7942_2466 at 2546.989 kb on + strand, within Synpcc7942_2466 at 2546.989 kb on + strand, within Synpcc7942_2466 at 2546.997 kb on - strand, within Synpcc7942_2466 at 2546.997 kb on - strand, within Synpcc7942_2466 at 2546.997 kb on - strand, within Synpcc7942_2466 at 2546.997 kb on - strand, within Synpcc7942_2466 at 2546.997 kb on - strand, within Synpcc7942_2466 at 2546.997 kb on - strand, within Synpcc7942_2466 at 2547.023 kb on + strand, within Synpcc7942_2466 at 2547.034 kb on + strand, within Synpcc7942_2466 at 2547.034 kb on + strand, within Synpcc7942_2466 at 2547.036 kb on + strand, within Synpcc7942_2466 at 2547.042 kb on - strand at 2547.042 kb on - strand, within Synpcc7942_2466 at 2547.042 kb on - strand, within Synpcc7942_2466 at 2547.068 kb on - strand, within Synpcc7942_2466 at 2547.134 kb on - strand, within Synpcc7942_2466 at 2547.163 kb on + strand at 2547.201 kb on + strand, within Synpcc7942_2466 at 2547.201 kb on + strand, within Synpcc7942_2466 at 2547.225 kb on + strand, within Synpcc7942_2466 at 2547.235 kb on + strand, within Synpcc7942_2466 at 2547.285 kb on + strand, within Synpcc7942_2466 at 2547.285 kb on + strand, within Synpcc7942_2466 at 2547.285 kb on - strand, within Synpcc7942_2466 at 2547.293 kb on - strand, within Synpcc7942_2466 at 2547.312 kb on + strand, within Synpcc7942_2466 at 2547.312 kb on + strand, within Synpcc7942_2466 at 2547.320 kb on - strand, within Synpcc7942_2466 at 2547.337 kb on - strand, within Synpcc7942_2466 at 2547.348 kb on + strand, within Synpcc7942_2466 at 2547.357 kb on - strand, within Synpcc7942_2466 at 2547.357 kb on - strand, within Synpcc7942_2466 at 2547.359 kb on + strand, within Synpcc7942_2466 at 2547.360 kb on + strand, within Synpcc7942_2466 at 2547.360 kb on + strand, within Synpcc7942_2466 at 2547.360 kb on + strand, within Synpcc7942_2466 at 2547.360 kb on + strand, within Synpcc7942_2466 at 2547.360 kb on + strand, within Synpcc7942_2466 at 2547.360 kb on + strand, within Synpcc7942_2466 at 2547.360 kb on + strand, within Synpcc7942_2466 at 2547.360 kb on + strand, within Synpcc7942_2466 at 2547.360 kb on + strand, within Synpcc7942_2466 at 2547.360 kb on + strand, within Synpcc7942_2466 at 2547.360 kb on + strand, within Synpcc7942_2466 at 2547.360 kb on + strand, within Synpcc7942_2466 at 2547.360 kb on + strand, within Synpcc7942_2466 at 2547.360 kb on + strand, within Synpcc7942_2466 at 2547.360 kb on + strand, within Synpcc7942_2466 at 2547.360 kb on + strand, within Synpcc7942_2466 at 2547.367 kb on - strand, within Synpcc7942_2466 at 2547.368 kb on - strand, within Synpcc7942_2466 at 2547.368 kb on - strand, within Synpcc7942_2466 at 2547.378 kb on + strand, within Synpcc7942_2466 at 2547.434 kb on - strand, within Synpcc7942_2466 at 2547.436 kb on + strand, within Synpcc7942_2466 at 2547.454 kb on + strand, within Synpcc7942_2466 at 2547.454 kb on - strand, within Synpcc7942_2466 at 2547.534 kb on - strand at 2547.575 kb on + strand at 2547.575 kb on + strand at 2547.583 kb on - strand at 2547.583 kb on - strand at 2548.440 kb on + strand at 2548.452 kb on - strand at 2548.459 kb on - strand at 2548.472 kb on + strand at 2548.480 kb on - strand at 2548.480 kb on - strand at 2548.480 kb on - strand at 2548.480 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.0625 mg/ml remove 2,545,853 + Synpcc7942_2464 0.55 -1.1 2,545,866 - Synpcc7942_2464 0.57 +3.3 2,545,907 + Synpcc7942_2464 0.63 +0.3 2,545,924 - Synpcc7942_2464 0.65 +0.1 2,545,925 - Synpcc7942_2464 0.65 -1.7 2,545,925 - Synpcc7942_2464 0.65 +0.7 2,545,936 + Synpcc7942_2464 0.67 -1.4 2,545,944 - Synpcc7942_2464 0.68 +1.3 2,545,999 + Synpcc7942_2464 0.76 -2.3 2,546,007 + Synpcc7942_2464 0.77 +0.8 2,546,010 - Synpcc7942_2464 0.78 -1.7 2,546,015 - Synpcc7942_2464 0.78 +0.3 2,546,026 + Synpcc7942_2464 0.80 +1.3 2,546,044 - Synpcc7942_2464 0.82 +0.5 2,546,049 - Synpcc7942_2464 0.83 +2.5 2,546,054 - Synpcc7942_2464 0.84 -0.3 2,546,054 - Synpcc7942_2464 0.84 +1.4 2,546,076 + Synpcc7942_2464 0.87 +0.9 2,546,081 + Synpcc7942_2464 0.88 -1.4 2,546,130 - -1.3 2,546,132 + -0.7 2,546,139 - -2.1 2,546,180 - +0.8 2,546,181 - -1.0 2,546,189 + -0.1 2,546,197 - -0.3 2,546,230 + -0.7 2,546,231 + +0.7 2,546,239 - -3.0 2,546,255 + +0.2 2,546,361 + Synpcc7942_2465 0.10 -1.6 2,546,366 + Synpcc7942_2465 0.12 -0.3 2,546,373 + Synpcc7942_2465 0.15 -1.7 2,546,423 - Synpcc7942_2465 0.35 -0.7 2,546,483 + Synpcc7942_2465 0.59 -2.0 2,546,547 + Synpcc7942_2465 0.84 -2.7 2,546,547 + Synpcc7942_2465 0.84 +1.9 2,546,591 - -2.3 2,546,615 + +0.3 2,546,623 - -0.7 2,546,623 - -2.7 2,546,638 + -0.0 2,546,649 + -0.2 2,546,659 + -4.1 2,546,667 - -0.7 2,546,693 + -1.7 2,546,693 + -0.7 2,546,693 + -2.5 2,546,701 - +0.8 2,546,725 - -2.5 2,546,751 + +0.3 2,546,783 - +0.1 2,546,783 - +1.5 2,546,790 - -0.7 2,546,794 + +1.3 2,546,798 + -1.0 2,546,824 - -2.0 2,546,829 + -2.3 2,546,830 - +0.3 2,546,831 + -0.4 2,546,837 - -2.0 2,546,837 - +1.3 2,546,837 - +1.2 2,546,842 + +0.1 2,546,842 + +1.3 2,546,842 + -1.8 2,546,864 + +0.6 2,546,864 + +1.6 2,546,864 + -3.6 2,546,865 + -3.3 2,546,892 - -1.8 2,546,906 + +0.3 2,546,909 - +1.1 2,546,954 + Synpcc7942_2466 0.15 +0.3 2,546,954 + Synpcc7942_2466 0.15 -1.7 2,546,989 + Synpcc7942_2466 0.20 +0.0 2,546,989 + Synpcc7942_2466 0.20 -2.3 2,546,989 + Synpcc7942_2466 0.20 -1.7 2,546,989 + Synpcc7942_2466 0.20 +3.9 2,546,989 + Synpcc7942_2466 0.20 -1.2 2,546,997 - Synpcc7942_2466 0.21 +0.3 2,546,997 - Synpcc7942_2466 0.21 +0.7 2,546,997 - Synpcc7942_2466 0.21 -0.6 2,546,997 - Synpcc7942_2466 0.21 -1.7 2,546,997 - Synpcc7942_2466 0.21 -1.7 2,546,997 - Synpcc7942_2466 0.21 +0.3 2,547,023 + Synpcc7942_2466 0.24 -0.7 2,547,034 + Synpcc7942_2466 0.26 +0.3 2,547,034 + Synpcc7942_2466 0.26 -0.7 2,547,036 + Synpcc7942_2466 0.26 +0.3 2,547,042 - -1.3 2,547,042 - Synpcc7942_2466 0.27 +0.1 2,547,042 - Synpcc7942_2466 0.27 -2.0 2,547,068 - Synpcc7942_2466 0.31 +0.3 2,547,134 - Synpcc7942_2466 0.40 -0.3 2,547,163 + -0.7 2,547,201 + Synpcc7942_2466 0.50 -2.3 2,547,201 + Synpcc7942_2466 0.50 +0.9 2,547,225 + Synpcc7942_2466 0.53 -0.3 2,547,235 + Synpcc7942_2466 0.54 -1.0 2,547,285 + Synpcc7942_2466 0.61 +0.9 2,547,285 + Synpcc7942_2466 0.61 +1.8 2,547,285 - Synpcc7942_2466 0.61 +0.3 2,547,293 - Synpcc7942_2466 0.63 +0.8 2,547,312 + Synpcc7942_2466 0.65 +1.3 2,547,312 + Synpcc7942_2466 0.65 -0.1 2,547,320 - Synpcc7942_2466 0.66 -0.3 2,547,337 - Synpcc7942_2466 0.69 -0.9 2,547,348 + Synpcc7942_2466 0.70 -2.0 2,547,357 - Synpcc7942_2466 0.72 -3.2 2,547,357 - Synpcc7942_2466 0.72 -2.0 2,547,359 + Synpcc7942_2466 0.72 -1.5 2,547,360 + Synpcc7942_2466 0.72 -0.3 2,547,360 + Synpcc7942_2466 0.72 -0.5 2,547,360 + Synpcc7942_2466 0.72 -0.3 2,547,360 + Synpcc7942_2466 0.72 -0.5 2,547,360 + Synpcc7942_2466 0.72 -1.2 2,547,360 + Synpcc7942_2466 0.72 -2.5 2,547,360 + Synpcc7942_2466 0.72 +1.1 2,547,360 + Synpcc7942_2466 0.72 -1.7 2,547,360 + Synpcc7942_2466 0.72 -2.0 2,547,360 + Synpcc7942_2466 0.72 +1.7 2,547,360 + Synpcc7942_2466 0.72 +2.9 2,547,360 + Synpcc7942_2466 0.72 -3.1 2,547,360 + Synpcc7942_2466 0.72 -0.1 2,547,360 + Synpcc7942_2466 0.72 -0.7 2,547,360 + Synpcc7942_2466 0.72 -2.0 2,547,360 + Synpcc7942_2466 0.72 -0.8 2,547,367 - Synpcc7942_2466 0.73 -1.3 2,547,368 - Synpcc7942_2466 0.73 -1.3 2,547,368 - Synpcc7942_2466 0.73 +0.1 2,547,378 + Synpcc7942_2466 0.75 +0.3 2,547,434 - Synpcc7942_2466 0.82 -1.2 2,547,436 + Synpcc7942_2466 0.83 +1.4 2,547,454 + Synpcc7942_2466 0.85 -0.7 2,547,454 - Synpcc7942_2466 0.85 +0.4 2,547,534 - +0.1 2,547,575 + -1.0 2,547,575 + -0.6 2,547,583 - -0.0 2,547,583 - -0.7 2,548,440 + +0.3 2,548,452 - -2.3 2,548,459 - +1.3 2,548,472 + +1.6 2,548,480 - +2.2 2,548,480 - +0.2 2,548,480 - +0.9 2,548,480 - +0.3
Or see this region's nucleotide sequence