Experiment: D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaE and Synpcc7942_2200 are separated by 57 nucleotides Synpcc7942_2200 and tRNA-Ser are separated by 122 nucleotides tRNA-Ser and alr are separated by 38 nucleotides alr and mcrA are separated by 124 nucleotides mcrA and prfA are separated by 13 nucleotides
Synpcc7942_2199: dnaE - DNA polymerase III subunit alpha, at 2,277,916 to 2,280,528
dnaE
Synpcc7942_2200: Synpcc7942_2200 - hypothetical protein, at 2,280,586 to 2,281,077
_2200
Synpcc7942_R0008: tRNA-Ser - tRNA-Ser, at 2,281,200 to 2,281,288
tRNA-Ser
Synpcc7942_2201: alr - alanine racemase, at 2,281,327 to 2,282,547
alr
Synpcc7942_2202: mcrA - HNH nuclease, at 2,282,672 to 2,283,169
mcrA
Synpcc7942_2203: prfA - peptide chain release factor 1, at 2,283,183 to 2,284,289
prfA
Position (kb)
2281
2282
2283 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 2280.548 kb on - strand at 2280.553 kb on + strand at 2280.583 kb on - strand at 2280.588 kb on - strand at 2280.597 kb on - strand at 2280.635 kb on + strand at 2280.635 kb on + strand at 2280.692 kb on + strand, within Synpcc7942_2200 at 2280.713 kb on - strand, within Synpcc7942_2200 at 2280.715 kb on - strand, within Synpcc7942_2200 at 2280.715 kb on - strand, within Synpcc7942_2200 at 2280.725 kb on - strand, within Synpcc7942_2200 at 2280.725 kb on - strand, within Synpcc7942_2200 at 2280.747 kb on + strand, within Synpcc7942_2200 at 2280.749 kb on + strand, within Synpcc7942_2200 at 2280.757 kb on - strand, within Synpcc7942_2200 at 2280.767 kb on - strand, within Synpcc7942_2200 at 2280.837 kb on - strand, within Synpcc7942_2200 at 2280.841 kb on + strand, within Synpcc7942_2200 at 2280.844 kb on - strand, within Synpcc7942_2200 at 2280.893 kb on + strand, within Synpcc7942_2200 at 2280.909 kb on + strand, within Synpcc7942_2200 at 2280.917 kb on - strand, within Synpcc7942_2200 at 2280.917 kb on - strand, within Synpcc7942_2200 at 2280.917 kb on - strand, within Synpcc7942_2200 at 2280.952 kb on + strand, within Synpcc7942_2200 at 2280.960 kb on - strand, within Synpcc7942_2200 at 2280.964 kb on - strand, within Synpcc7942_2200 at 2280.964 kb on - strand, within Synpcc7942_2200 at 2280.966 kb on + strand, within Synpcc7942_2200 at 2280.971 kb on + strand, within Synpcc7942_2200 at 2280.974 kb on - strand, within Synpcc7942_2200 at 2280.976 kb on + strand, within Synpcc7942_2200 at 2280.979 kb on - strand, within Synpcc7942_2200 at 2281.054 kb on - strand at 2281.082 kb on + strand at 2281.088 kb on + strand at 2281.090 kb on - strand at 2281.162 kb on + strand at 2281.326 kb on + strand at 2281.334 kb on - strand at 2282.504 kb on + strand at 2282.535 kb on + strand at 2282.557 kb on + strand at 2282.557 kb on + strand at 2282.557 kb on + strand at 2282.565 kb on - strand at 2282.587 kb on - strand at 2282.599 kb on - strand at 2282.608 kb on - strand at 2282.628 kb on + strand at 2282.681 kb on + strand at 2282.681 kb on - strand at 2282.708 kb on + strand at 2282.769 kb on + strand, within mcrA at 2282.777 kb on - strand, within mcrA at 2282.839 kb on - strand, within mcrA at 2282.864 kb on + strand, within mcrA at 2282.864 kb on + strand at 2282.865 kb on + strand, within mcrA at 2282.911 kb on + strand, within mcrA at 2283.059 kb on + strand, within mcrA at 2283.059 kb on + strand, within mcrA at 2283.064 kb on + strand, within mcrA at 2283.064 kb on + strand, within mcrA at 2283.067 kb on - strand at 2283.072 kb on - strand, within mcrA at 2283.072 kb on - strand, within mcrA at 2283.084 kb on - strand, within mcrA at 2283.117 kb on + strand, within mcrA at 2283.125 kb on - strand at 2283.181 kb on + strand at 2283.184 kb on - strand at 2283.189 kb on - strand at 2283.198 kb on + strand at 2283.208 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.0625 mg/ml remove 2,280,548 - -2.3 2,280,553 + -1.3 2,280,583 - -1.3 2,280,588 - -1.9 2,280,597 - +0.3 2,280,635 + +1.3 2,280,635 + -1.3 2,280,692 + Synpcc7942_2200 0.22 -0.2 2,280,713 - Synpcc7942_2200 0.26 +1.1 2,280,715 - Synpcc7942_2200 0.26 +1.5 2,280,715 - Synpcc7942_2200 0.26 -0.4 2,280,725 - Synpcc7942_2200 0.28 -0.0 2,280,725 - Synpcc7942_2200 0.28 -0.7 2,280,747 + Synpcc7942_2200 0.33 +0.3 2,280,749 + Synpcc7942_2200 0.33 -0.0 2,280,757 - Synpcc7942_2200 0.35 -0.7 2,280,767 - Synpcc7942_2200 0.37 +0.7 2,280,837 - Synpcc7942_2200 0.51 -0.1 2,280,841 + Synpcc7942_2200 0.52 -2.5 2,280,844 - Synpcc7942_2200 0.52 -1.7 2,280,893 + Synpcc7942_2200 0.62 +1.3 2,280,909 + Synpcc7942_2200 0.66 -0.3 2,280,917 - Synpcc7942_2200 0.67 +0.3 2,280,917 - Synpcc7942_2200 0.67 +2.3 2,280,917 - Synpcc7942_2200 0.67 +2.9 2,280,952 + Synpcc7942_2200 0.74 -2.3 2,280,960 - Synpcc7942_2200 0.76 -2.0 2,280,964 - Synpcc7942_2200 0.77 +1.4 2,280,964 - Synpcc7942_2200 0.77 +2.3 2,280,966 + Synpcc7942_2200 0.77 -0.3 2,280,971 + Synpcc7942_2200 0.78 +2.5 2,280,974 - Synpcc7942_2200 0.79 +0.4 2,280,976 + Synpcc7942_2200 0.79 -0.7 2,280,979 - Synpcc7942_2200 0.80 +0.5 2,281,054 - +0.6 2,281,082 + +0.2 2,281,088 + -1.6 2,281,090 - -0.5 2,281,162 + +1.0 2,281,326 + -3.9 2,281,334 - +0.3 2,282,504 + -5.8 2,282,535 + +0.3 2,282,557 + +2.6 2,282,557 + +0.1 2,282,557 + -0.2 2,282,565 - -3.0 2,282,587 - -3.3 2,282,599 - -1.3 2,282,608 - -2.7 2,282,628 + +0.3 2,282,681 + +0.4 2,282,681 - +1.3 2,282,708 + +0.4 2,282,769 + mcrA Synpcc7942_2202 0.19 -2.5 2,282,777 - mcrA Synpcc7942_2202 0.21 -1.2 2,282,839 - mcrA Synpcc7942_2202 0.34 -2.0 2,282,864 + mcrA Synpcc7942_2202 0.39 -1.7 2,282,864 + -1.3 2,282,865 + mcrA Synpcc7942_2202 0.39 +3.6 2,282,911 + mcrA Synpcc7942_2202 0.48 -0.1 2,283,059 + mcrA Synpcc7942_2202 0.78 -0.7 2,283,059 + mcrA Synpcc7942_2202 0.78 +0.6 2,283,064 + mcrA Synpcc7942_2202 0.79 -2.5 2,283,064 + mcrA Synpcc7942_2202 0.79 -2.0 2,283,067 - +0.3 2,283,072 - mcrA Synpcc7942_2202 0.80 -1.1 2,283,072 - mcrA Synpcc7942_2202 0.80 -1.0 2,283,084 - mcrA Synpcc7942_2202 0.83 +1.4 2,283,117 + mcrA Synpcc7942_2202 0.89 -1.7 2,283,125 - +3.2 2,283,181 + +1.9 2,283,184 - +0.9 2,283,189 - +1.4 2,283,198 + -2.5 2,283,208 + -2.0
Or see this region's nucleotide sequence