Experiment: D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpC and phdD are separated by 76 nucleotides phdD and Synpcc7942_1199 are separated by 58 nucleotides Synpcc7942_1199 and Synpcc7942_1200 are separated by 40 nucleotides
Synpcc7942_1197: trpC - indole-3-glycerol-phosphate synthase, at 1,224,841 to 1,225,728
trpC
Synpcc7942_1198: phdD - dihydrolipoamide dehydrogenase, at 1,225,805 to 1,227,244
phdD
Synpcc7942_1199: Synpcc7942_1199 - probable tRNA/rRNA methyltransferase, at 1,227,303 to 1,228,007
_1199
Synpcc7942_1200: Synpcc7942_1200 - hypothetical protein, at 1,228,048 to 1,229,061
_1200
Position (kb)
1225
1226
1227
1228 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1224.839 kb on + strand at 1224.839 kb on + strand at 1224.842 kb on - strand at 1224.846 kb on - strand at 1224.846 kb on - strand at 1224.847 kb on - strand at 1224.847 kb on - strand at 1224.853 kb on - strand at 1224.871 kb on + strand at 1225.738 kb on + strand at 1225.795 kb on + strand at 1225.832 kb on - strand at 1227.257 kb on - strand at 1227.333 kb on + strand at 1227.333 kb on + strand at 1227.363 kb on - strand at 1227.422 kb on + strand, within Synpcc7942_1199 at 1227.422 kb on + strand, within Synpcc7942_1199 at 1227.422 kb on + strand, within Synpcc7942_1199 at 1227.427 kb on + strand, within Synpcc7942_1199 at 1227.430 kb on - strand, within Synpcc7942_1199 at 1227.431 kb on - strand, within Synpcc7942_1199 at 1227.431 kb on - strand, within Synpcc7942_1199 at 1227.434 kb on + strand, within Synpcc7942_1199 at 1227.442 kb on - strand, within Synpcc7942_1199 at 1227.442 kb on - strand, within Synpcc7942_1199 at 1227.479 kb on + strand, within Synpcc7942_1199 at 1227.482 kb on - strand, within Synpcc7942_1199 at 1227.482 kb on - strand, within Synpcc7942_1199 at 1227.513 kb on - strand, within Synpcc7942_1199 at 1227.537 kb on + strand, within Synpcc7942_1199 at 1227.543 kb on - strand, within Synpcc7942_1199 at 1227.551 kb on + strand, within Synpcc7942_1199 at 1227.557 kb on + strand, within Synpcc7942_1199 at 1227.559 kb on - strand, within Synpcc7942_1199 at 1227.564 kb on + strand, within Synpcc7942_1199 at 1227.569 kb on + strand, within Synpcc7942_1199 at 1227.572 kb on - strand, within Synpcc7942_1199 at 1227.618 kb on + strand, within Synpcc7942_1199 at 1227.625 kb on + strand, within Synpcc7942_1199 at 1227.641 kb on + strand, within Synpcc7942_1199 at 1227.694 kb on + strand, within Synpcc7942_1199 at 1227.755 kb on + strand, within Synpcc7942_1199 at 1227.763 kb on - strand, within Synpcc7942_1199 at 1227.783 kb on - strand, within Synpcc7942_1199 at 1227.785 kb on + strand, within Synpcc7942_1199 at 1227.790 kb on + strand, within Synpcc7942_1199 at 1227.800 kb on + strand, within Synpcc7942_1199 at 1227.800 kb on + strand, within Synpcc7942_1199 at 1227.808 kb on - strand, within Synpcc7942_1199 at 1227.808 kb on - strand, within Synpcc7942_1199 at 1227.834 kb on - strand, within Synpcc7942_1199 at 1227.834 kb on - strand, within Synpcc7942_1199 at 1227.839 kb on + strand, within Synpcc7942_1199 at 1227.839 kb on + strand, within Synpcc7942_1199 at 1227.928 kb on + strand, within Synpcc7942_1199 at 1227.990 kb on - strand at 1227.990 kb on - strand at 1227.993 kb on - strand at 1228.026 kb on - strand at 1228.026 kb on - strand at 1228.026 kb on - strand at 1228.026 kb on - strand at 1228.079 kb on - strand at 1228.082 kb on + strand at 1228.082 kb on + strand at 1228.082 kb on + strand at 1228.082 kb on + strand at 1228.082 kb on + strand at 1228.084 kb on - strand at 1228.084 kb on - strand at 1228.090 kb on - strand at 1228.090 kb on - strand at 1228.092 kb on - strand at 1228.116 kb on + strand at 1228.132 kb on + strand at 1228.145 kb on - strand at 1228.234 kb on + strand, within Synpcc7942_1200
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.0625 mg/ml remove 1,224,839 + +0.1 1,224,839 + -0.7 1,224,842 - -0.7 1,224,846 - +0.5 1,224,846 - -2.6 1,224,847 - -2.4 1,224,847 - +1.2 1,224,853 - +2.4 1,224,871 + -0.7 1,225,738 + +0.1 1,225,795 + +0.6 1,225,832 - +1.2 1,227,257 - +0.3 1,227,333 + -0.6 1,227,333 + -1.7 1,227,363 - -0.8 1,227,422 + Synpcc7942_1199 0.17 +1.5 1,227,422 + Synpcc7942_1199 0.17 +3.1 1,227,422 + Synpcc7942_1199 0.17 +1.5 1,227,427 + Synpcc7942_1199 0.18 +0.9 1,227,430 - Synpcc7942_1199 0.18 -1.0 1,227,431 - Synpcc7942_1199 0.18 +1.1 1,227,431 - Synpcc7942_1199 0.18 -0.7 1,227,434 + Synpcc7942_1199 0.19 -1.7 1,227,442 - Synpcc7942_1199 0.20 -3.5 1,227,442 - Synpcc7942_1199 0.20 -0.7 1,227,479 + Synpcc7942_1199 0.25 +3.8 1,227,482 - Synpcc7942_1199 0.25 -0.5 1,227,482 - Synpcc7942_1199 0.25 -1.7 1,227,513 - Synpcc7942_1199 0.30 -1.7 1,227,537 + Synpcc7942_1199 0.33 -0.7 1,227,543 - Synpcc7942_1199 0.34 -0.9 1,227,551 + Synpcc7942_1199 0.35 -1.3 1,227,557 + Synpcc7942_1199 0.36 +0.9 1,227,559 - Synpcc7942_1199 0.36 -0.6 1,227,564 + Synpcc7942_1199 0.37 +0.3 1,227,569 + Synpcc7942_1199 0.38 +0.1 1,227,572 - Synpcc7942_1199 0.38 +0.3 1,227,618 + Synpcc7942_1199 0.45 +1.2 1,227,625 + Synpcc7942_1199 0.46 -1.0 1,227,641 + Synpcc7942_1199 0.48 +1.4 1,227,694 + Synpcc7942_1199 0.55 +0.9 1,227,755 + Synpcc7942_1199 0.64 +0.7 1,227,763 - Synpcc7942_1199 0.65 +0.7 1,227,783 - Synpcc7942_1199 0.68 -1.3 1,227,785 + Synpcc7942_1199 0.68 +1.3 1,227,790 + Synpcc7942_1199 0.69 +4.6 1,227,800 + Synpcc7942_1199 0.70 +2.2 1,227,800 + Synpcc7942_1199 0.70 -0.7 1,227,808 - Synpcc7942_1199 0.72 +0.5 1,227,808 - Synpcc7942_1199 0.72 -2.5 1,227,834 - Synpcc7942_1199 0.75 -2.0 1,227,834 - Synpcc7942_1199 0.75 -0.5 1,227,839 + Synpcc7942_1199 0.76 -0.3 1,227,839 + Synpcc7942_1199 0.76 -2.5 1,227,928 + Synpcc7942_1199 0.89 -0.8 1,227,990 - +0.3 1,227,990 - -0.7 1,227,993 - -3.0 1,228,026 - -1.3 1,228,026 - +0.1 1,228,026 - -0.2 1,228,026 - -2.0 1,228,079 - -1.1 1,228,082 + -1.3 1,228,082 + -0.7 1,228,082 + +0.3 1,228,082 + +0.3 1,228,082 + -0.3 1,228,084 - +1.6 1,228,084 - +0.4 1,228,090 - +1.9 1,228,090 - +0.3 1,228,092 - -0.7 1,228,116 + -0.4 1,228,132 + +3.0 1,228,145 - +2.0 1,228,234 + Synpcc7942_1200 0.18 +0.9
Or see this region's nucleotide sequence