Experiment: D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aat and Synpcc7942_0607 are separated by 13 nucleotides Synpcc7942_0607 and Synpcc7942_0608 are separated by 48 nucleotides Synpcc7942_0608 and ndhD4 are separated by 83 nucleotides
Synpcc7942_0606: aat - leucyl/phenylalanyl-tRNA--protein transferase, at 594,687 to 595,367
aat
Synpcc7942_0607: Synpcc7942_0607 - hypothetical protein, at 595,381 to 595,707
_0607
Synpcc7942_0608: Synpcc7942_0608 - hypothetical protein, at 595,756 to 596,298
_0608
Synpcc7942_0609: ndhD4 - proton-translocating NADH-quinone oxidoreductase, chain M, at 596,382 to 597,872
ndhD4
Position (kb) 595
596
597 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 594.793 kb on + strand, within aat at 594.801 kb on - strand, within aat at 594.887 kb on - strand, within aat at 595.026 kb on - strand, within aat at 595.031 kb on - strand, within aat at 595.063 kb on - strand, within aat at 595.095 kb on - strand, within aat at 595.152 kb on - strand, within aat at 595.242 kb on + strand, within aat at 595.252 kb on + strand, within aat at 595.255 kb on - strand, within aat at 595.260 kb on - strand, within aat at 595.270 kb on + strand, within aat at 595.403 kb on - strand at 595.458 kb on + strand, within Synpcc7942_0607 at 595.458 kb on + strand, within Synpcc7942_0607 at 595.466 kb on - strand, within Synpcc7942_0607 at 595.471 kb on + strand, within Synpcc7942_0607 at 595.476 kb on - strand, within Synpcc7942_0607 at 595.479 kb on - strand, within Synpcc7942_0607 at 595.537 kb on + strand, within Synpcc7942_0607 at 595.558 kb on + strand, within Synpcc7942_0607 at 595.599 kb on - strand, within Synpcc7942_0607 at 595.603 kb on - strand, within Synpcc7942_0607 at 595.689 kb on - strand at 595.695 kb on + strand at 595.734 kb on - strand at 595.762 kb on + strand at 595.786 kb on - strand at 595.791 kb on - strand at 595.805 kb on - strand at 595.827 kb on + strand, within Synpcc7942_0608 at 595.859 kb on + strand, within Synpcc7942_0608 at 595.973 kb on + strand, within Synpcc7942_0608 at 595.990 kb on - strand, within Synpcc7942_0608 at 595.990 kb on - strand, within Synpcc7942_0608 at 596.012 kb on + strand, within Synpcc7942_0608 at 596.039 kb on - strand, within Synpcc7942_0608 at 596.052 kb on - strand, within Synpcc7942_0608 at 596.103 kb on + strand, within Synpcc7942_0608 at 596.103 kb on + strand, within Synpcc7942_0608 at 596.103 kb on + strand, within Synpcc7942_0608 at 596.118 kb on + strand, within Synpcc7942_0608 at 596.125 kb on + strand, within Synpcc7942_0608 at 596.125 kb on + strand, within Synpcc7942_0608 at 596.125 kb on + strand, within Synpcc7942_0608 at 596.125 kb on + strand, within Synpcc7942_0608 at 596.125 kb on + strand, within Synpcc7942_0608 at 596.125 kb on + strand, within Synpcc7942_0608 at 596.126 kb on - strand, within Synpcc7942_0608 at 596.130 kb on + strand, within Synpcc7942_0608 at 596.133 kb on - strand, within Synpcc7942_0608 at 596.133 kb on - strand, within Synpcc7942_0608 at 596.133 kb on - strand, within Synpcc7942_0608 at 596.133 kb on - strand, within Synpcc7942_0608 at 596.133 kb on - strand, within Synpcc7942_0608 at 596.133 kb on - strand, within Synpcc7942_0608 at 596.133 kb on - strand, within Synpcc7942_0608 at 596.180 kb on + strand, within Synpcc7942_0608 at 596.188 kb on - strand at 596.199 kb on + strand, within Synpcc7942_0608 at 596.207 kb on + strand, within Synpcc7942_0608 at 596.207 kb on - strand, within Synpcc7942_0608 at 596.212 kb on - strand, within Synpcc7942_0608 at 596.213 kb on - strand, within Synpcc7942_0608 at 596.213 kb on - strand, within Synpcc7942_0608 at 596.288 kb on - strand at 596.313 kb on + strand at 596.320 kb on + strand at 596.320 kb on + strand at 596.320 kb on + strand at 596.320 kb on + strand at 596.320 kb on + strand at 596.328 kb on - strand at 596.328 kb on - strand at 596.328 kb on - strand at 596.328 kb on - strand at 596.332 kb on + strand at 596.332 kb on + strand at 596.366 kb on - strand at 596.366 kb on - strand at 596.387 kb on - strand at 596.437 kb on + strand at 596.445 kb on - strand at 596.445 kb on - strand at 596.452 kb on + strand at 596.461 kb on + strand at 596.570 kb on + strand, within ndhD4 at 596.570 kb on + strand, within ndhD4 at 596.578 kb on - strand, within ndhD4 at 596.578 kb on - strand, within ndhD4 at 596.578 kb on - strand, within ndhD4 at 596.592 kb on - strand, within ndhD4 at 596.592 kb on - strand, within ndhD4 at 596.625 kb on - strand at 596.638 kb on + strand, within ndhD4 at 596.647 kb on - strand, within ndhD4 at 596.647 kb on - strand, within ndhD4 at 596.648 kb on + strand, within ndhD4 at 596.649 kb on + strand, within ndhD4 at 596.649 kb on + strand, within ndhD4 at 596.649 kb on + strand, within ndhD4 at 596.683 kb on + strand, within ndhD4 at 596.705 kb on + strand, within ndhD4 at 596.708 kb on - strand, within ndhD4 at 596.713 kb on - strand, within ndhD4 at 596.741 kb on + strand, within ndhD4 at 596.749 kb on - strand, within ndhD4 at 596.749 kb on - strand, within ndhD4 at 596.764 kb on + strand, within ndhD4 at 596.772 kb on - strand, within ndhD4 at 596.772 kb on - strand, within ndhD4 at 596.772 kb on - strand, within ndhD4 at 596.777 kb on - strand, within ndhD4 at 596.807 kb on + strand, within ndhD4 at 596.888 kb on - strand, within ndhD4 at 596.888 kb on - strand, within ndhD4 at 596.890 kb on - strand, within ndhD4 at 596.975 kb on + strand, within ndhD4 at 596.981 kb on - strand, within ndhD4 at 596.991 kb on - strand, within ndhD4 at 597.019 kb on - strand, within ndhD4 at 597.104 kb on - strand, within ndhD4 at 597.110 kb on + strand, within ndhD4 at 597.144 kb on + strand, within ndhD4 at 597.185 kb on - strand, within ndhD4 at 597.228 kb on + strand, within ndhD4 at 597.228 kb on + strand, within ndhD4 at 597.236 kb on - strand, within ndhD4 at 597.238 kb on - strand at 597.242 kb on + strand, within ndhD4
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.0625 mg/ml remove 594,793 + aat Synpcc7942_0606 0.16 +0.3 594,801 - aat Synpcc7942_0606 0.17 +2.2 594,887 - aat Synpcc7942_0606 0.29 -1.9 595,026 - aat Synpcc7942_0606 0.50 -1.3 595,031 - aat Synpcc7942_0606 0.51 +0.6 595,063 - aat Synpcc7942_0606 0.55 +0.3 595,095 - aat Synpcc7942_0606 0.60 +0.8 595,152 - aat Synpcc7942_0606 0.68 -0.8 595,242 + aat Synpcc7942_0606 0.81 -2.9 595,252 + aat Synpcc7942_0606 0.83 -1.6 595,255 - aat Synpcc7942_0606 0.83 -3.3 595,260 - aat Synpcc7942_0606 0.84 +2.0 595,270 + aat Synpcc7942_0606 0.86 +0.9 595,403 - -2.1 595,458 + Synpcc7942_0607 0.24 -1.7 595,458 + Synpcc7942_0607 0.24 +2.3 595,466 - Synpcc7942_0607 0.26 -3.1 595,471 + Synpcc7942_0607 0.28 -0.1 595,476 - Synpcc7942_0607 0.29 -1.5 595,479 - Synpcc7942_0607 0.30 +0.9 595,537 + Synpcc7942_0607 0.48 +1.9 595,558 + Synpcc7942_0607 0.54 +3.6 595,599 - Synpcc7942_0607 0.67 +1.2 595,603 - Synpcc7942_0607 0.68 -0.3 595,689 - +0.7 595,695 + +0.3 595,734 - +0.5 595,762 + -2.4 595,786 - -0.9 595,791 - -2.7 595,805 - -2.1 595,827 + Synpcc7942_0608 0.13 -1.7 595,859 + Synpcc7942_0608 0.19 -2.5 595,973 + Synpcc7942_0608 0.40 -2.2 595,990 - Synpcc7942_0608 0.43 +0.3 595,990 - Synpcc7942_0608 0.43 -3.8 596,012 + Synpcc7942_0608 0.47 -3.5 596,039 - Synpcc7942_0608 0.52 -2.3 596,052 - Synpcc7942_0608 0.55 -3.6 596,103 + Synpcc7942_0608 0.64 -1.0 596,103 + Synpcc7942_0608 0.64 -2.1 596,103 + Synpcc7942_0608 0.64 -3.6 596,118 + Synpcc7942_0608 0.67 -1.3 596,125 + Synpcc7942_0608 0.68 +0.9 596,125 + Synpcc7942_0608 0.68 -1.3 596,125 + Synpcc7942_0608 0.68 -1.7 596,125 + Synpcc7942_0608 0.68 -3.2 596,125 + Synpcc7942_0608 0.68 +0.7 596,125 + Synpcc7942_0608 0.68 -1.7 596,126 - Synpcc7942_0608 0.68 -0.3 596,130 + Synpcc7942_0608 0.69 -1.7 596,133 - Synpcc7942_0608 0.69 +0.9 596,133 - Synpcc7942_0608 0.69 -2.1 596,133 - Synpcc7942_0608 0.69 -4.1 596,133 - Synpcc7942_0608 0.69 -2.2 596,133 - Synpcc7942_0608 0.69 -0.7 596,133 - Synpcc7942_0608 0.69 -2.7 596,133 - Synpcc7942_0608 0.69 +0.3 596,180 + Synpcc7942_0608 0.78 -0.3 596,188 - +0.3 596,199 + Synpcc7942_0608 0.82 -3.8 596,207 + Synpcc7942_0608 0.83 -2.6 596,207 - Synpcc7942_0608 0.83 +0.3 596,212 - Synpcc7942_0608 0.84 -2.8 596,213 - Synpcc7942_0608 0.84 -2.9 596,213 - Synpcc7942_0608 0.84 -2.4 596,288 - +0.3 596,313 + -1.1 596,320 + +1.4 596,320 + +0.3 596,320 + -0.7 596,320 + -0.8 596,320 + +1.3 596,328 - -2.3 596,328 - -0.5 596,328 - +1.9 596,328 - -2.5 596,332 + -1.0 596,332 + +2.3 596,366 - +2.8 596,366 - +3.1 596,387 - +1.3 596,437 + +1.3 596,445 - +1.0 596,445 - -0.7 596,452 + +2.6 596,461 + +1.5 596,570 + ndhD4 Synpcc7942_0609 0.13 +1.0 596,570 + ndhD4 Synpcc7942_0609 0.13 -2.1 596,578 - ndhD4 Synpcc7942_0609 0.13 -1.1 596,578 - ndhD4 Synpcc7942_0609 0.13 -2.4 596,578 - ndhD4 Synpcc7942_0609 0.13 +2.3 596,592 - ndhD4 Synpcc7942_0609 0.14 -2.9 596,592 - ndhD4 Synpcc7942_0609 0.14 +2.9 596,625 - +2.7 596,638 + ndhD4 Synpcc7942_0609 0.17 +0.3 596,647 - ndhD4 Synpcc7942_0609 0.18 -2.1 596,647 - ndhD4 Synpcc7942_0609 0.18 +2.1 596,648 + ndhD4 Synpcc7942_0609 0.18 -0.7 596,649 + ndhD4 Synpcc7942_0609 0.18 +2.3 596,649 + ndhD4 Synpcc7942_0609 0.18 -0.7 596,649 + ndhD4 Synpcc7942_0609 0.18 -2.1 596,683 + ndhD4 Synpcc7942_0609 0.20 -0.1 596,705 + ndhD4 Synpcc7942_0609 0.22 +0.3 596,708 - ndhD4 Synpcc7942_0609 0.22 +1.1 596,713 - ndhD4 Synpcc7942_0609 0.22 +1.9 596,741 + ndhD4 Synpcc7942_0609 0.24 +3.6 596,749 - ndhD4 Synpcc7942_0609 0.25 +4.9 596,749 - ndhD4 Synpcc7942_0609 0.25 +0.3 596,764 + ndhD4 Synpcc7942_0609 0.26 +3.3 596,772 - ndhD4 Synpcc7942_0609 0.26 +3.4 596,772 - ndhD4 Synpcc7942_0609 0.26 -2.3 596,772 - ndhD4 Synpcc7942_0609 0.26 +0.3 596,777 - ndhD4 Synpcc7942_0609 0.26 +0.3 596,807 + ndhD4 Synpcc7942_0609 0.29 +0.3 596,888 - ndhD4 Synpcc7942_0609 0.34 -0.7 596,888 - ndhD4 Synpcc7942_0609 0.34 +4.3 596,890 - ndhD4 Synpcc7942_0609 0.34 -0.1 596,975 + ndhD4 Synpcc7942_0609 0.40 +1.8 596,981 - ndhD4 Synpcc7942_0609 0.40 +2.0 596,991 - ndhD4 Synpcc7942_0609 0.41 -1.7 597,019 - ndhD4 Synpcc7942_0609 0.43 +3.2 597,104 - ndhD4 Synpcc7942_0609 0.48 +0.3 597,110 + ndhD4 Synpcc7942_0609 0.49 +1.0 597,144 + ndhD4 Synpcc7942_0609 0.51 +2.6 597,185 - ndhD4 Synpcc7942_0609 0.54 +1.1 597,228 + ndhD4 Synpcc7942_0609 0.57 +1.1 597,228 + ndhD4 Synpcc7942_0609 0.57 +3.1 597,236 - ndhD4 Synpcc7942_0609 0.57 -1.3 597,238 - -0.7 597,242 + ndhD4 Synpcc7942_0609 0.58 +0.3
Or see this region's nucleotide sequence