Experiment: D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lipA and Synpcc7942_0543 overlap by 4 nucleotides Synpcc7942_0543 and Synpcc7942_0544 are separated by 78 nucleotides Synpcc7942_0544 and Synpcc7942_0545 overlap by 19 nucleotides Synpcc7942_0545 and nha4 are separated by 40 nucleotides
Synpcc7942_0542: lipA - lipoyl synthase, at 525,360 to 526,253
lipA
Synpcc7942_0543: Synpcc7942_0543 - hypothetical protein, at 526,250 to 526,813
_0543
Synpcc7942_0544: Synpcc7942_0544 - YciI-like protein, at 526,892 to 527,161
_0544
Synpcc7942_0545: Synpcc7942_0545 - hypothetical protein, at 527,143 to 527,835
_0545
Synpcc7942_0546: nha4 - Na+/H+ antiporter, at 527,876 to 530,023
nha4
Position (kb) 526
527
528 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 526.266 kb on - strand at 526.268 kb on + strand at 526.328 kb on + strand, within Synpcc7942_0543 at 526.358 kb on + strand, within Synpcc7942_0543 at 526.361 kb on - strand, within Synpcc7942_0543 at 526.361 kb on - strand, within Synpcc7942_0543 at 526.395 kb on + strand, within Synpcc7942_0543 at 526.403 kb on - strand, within Synpcc7942_0543 at 526.418 kb on + strand, within Synpcc7942_0543 at 526.426 kb on - strand, within Synpcc7942_0543 at 526.439 kb on + strand, within Synpcc7942_0543 at 526.439 kb on + strand at 526.498 kb on - strand, within Synpcc7942_0543 at 526.505 kb on + strand, within Synpcc7942_0543 at 526.505 kb on + strand, within Synpcc7942_0543 at 526.518 kb on - strand, within Synpcc7942_0543 at 526.532 kb on - strand, within Synpcc7942_0543 at 526.552 kb on + strand, within Synpcc7942_0543 at 526.565 kb on + strand, within Synpcc7942_0543 at 526.573 kb on - strand, within Synpcc7942_0543 at 526.573 kb on - strand, within Synpcc7942_0543 at 526.573 kb on - strand, within Synpcc7942_0543 at 526.588 kb on - strand, within Synpcc7942_0543 at 526.644 kb on - strand, within Synpcc7942_0543 at 526.646 kb on - strand, within Synpcc7942_0543 at 526.651 kb on - strand, within Synpcc7942_0543 at 526.689 kb on - strand, within Synpcc7942_0543 at 526.689 kb on - strand, within Synpcc7942_0543 at 526.711 kb on + strand, within Synpcc7942_0543 at 526.764 kb on + strand at 526.769 kb on + strand at 526.770 kb on - strand at 526.770 kb on - strand at 526.774 kb on + strand at 526.782 kb on - strand at 526.803 kb on + strand at 526.804 kb on - strand at 526.804 kb on - strand at 526.820 kb on + strand at 526.876 kb on + strand at 526.877 kb on + strand at 526.915 kb on - strand at 526.921 kb on + strand, within Synpcc7942_0544 at 526.982 kb on + strand, within Synpcc7942_0544 at 526.990 kb on - strand, within Synpcc7942_0544 at 526.997 kb on + strand at 526.999 kb on - strand, within Synpcc7942_0544 at 527.000 kb on + strand, within Synpcc7942_0544 at 527.002 kb on + strand, within Synpcc7942_0544 at 527.022 kb on + strand, within Synpcc7942_0544 at 527.022 kb on + strand, within Synpcc7942_0544 at 527.022 kb on + strand, within Synpcc7942_0544 at 527.022 kb on + strand, within Synpcc7942_0544 at 527.022 kb on + strand, within Synpcc7942_0544 at 527.022 kb on + strand, within Synpcc7942_0544 at 527.022 kb on + strand, within Synpcc7942_0544 at 527.027 kb on + strand, within Synpcc7942_0544 at 527.030 kb on - strand, within Synpcc7942_0544 at 527.030 kb on - strand, within Synpcc7942_0544 at 527.035 kb on - strand, within Synpcc7942_0544 at 527.035 kb on - strand, within Synpcc7942_0544 at 527.081 kb on - strand, within Synpcc7942_0544 at 527.081 kb on - strand, within Synpcc7942_0544 at 527.126 kb on + strand, within Synpcc7942_0544 at 527.135 kb on + strand at 527.135 kb on - strand at 527.135 kb on - strand at 527.207 kb on - strand at 527.207 kb on - strand at 527.207 kb on - strand at 527.207 kb on - strand at 527.207 kb on - strand at 527.207 kb on - strand at 527.209 kb on + strand at 527.209 kb on + strand at 527.209 kb on + strand at 527.209 kb on + strand at 527.209 kb on + strand at 527.209 kb on + strand at 527.209 kb on + strand at 527.209 kb on + strand at 527.217 kb on - strand, within Synpcc7942_0545 at 527.217 kb on - strand, within Synpcc7942_0545 at 527.246 kb on - strand, within Synpcc7942_0545 at 527.272 kb on - strand, within Synpcc7942_0545 at 527.282 kb on + strand, within Synpcc7942_0545 at 527.282 kb on + strand, within Synpcc7942_0545 at 527.290 kb on - strand, within Synpcc7942_0545 at 527.297 kb on + strand, within Synpcc7942_0545 at 527.307 kb on + strand, within Synpcc7942_0545 at 527.307 kb on + strand, within Synpcc7942_0545 at 527.307 kb on + strand, within Synpcc7942_0545 at 527.307 kb on + strand, within Synpcc7942_0545 at 527.307 kb on + strand, within Synpcc7942_0545 at 527.307 kb on + strand, within Synpcc7942_0545 at 527.313 kb on + strand, within Synpcc7942_0545 at 527.315 kb on - strand, within Synpcc7942_0545 at 527.315 kb on - strand, within Synpcc7942_0545 at 527.315 kb on - strand, within Synpcc7942_0545 at 527.315 kb on - strand, within Synpcc7942_0545 at 527.330 kb on - strand, within Synpcc7942_0545 at 527.347 kb on + strand, within Synpcc7942_0545 at 527.350 kb on - strand, within Synpcc7942_0545 at 527.355 kb on - strand, within Synpcc7942_0545 at 527.355 kb on - strand, within Synpcc7942_0545 at 527.382 kb on + strand, within Synpcc7942_0545 at 527.398 kb on - strand, within Synpcc7942_0545 at 527.441 kb on - strand, within Synpcc7942_0545 at 527.468 kb on - strand, within Synpcc7942_0545 at 527.516 kb on + strand, within Synpcc7942_0545 at 527.521 kb on + strand, within Synpcc7942_0545 at 527.527 kb on + strand, within Synpcc7942_0545 at 527.529 kb on - strand, within Synpcc7942_0545 at 527.590 kb on + strand, within Synpcc7942_0545 at 527.598 kb on + strand, within Synpcc7942_0545 at 527.598 kb on + strand, within Synpcc7942_0545 at 527.606 kb on - strand, within Synpcc7942_0545 at 527.629 kb on + strand, within Synpcc7942_0545 at 527.637 kb on - strand, within Synpcc7942_0545 at 527.653 kb on - strand, within Synpcc7942_0545 at 527.661 kb on - strand, within Synpcc7942_0545 at 527.716 kb on - strand, within Synpcc7942_0545 at 527.757 kb on - strand, within Synpcc7942_0545 at 527.767 kb on + strand at 527.775 kb on - strand at 527.784 kb on + strand at 527.796 kb on - strand at 527.811 kb on + strand at 527.819 kb on - strand at 527.845 kb on + strand at 527.845 kb on + strand at 527.845 kb on + strand at 527.852 kb on - strand at 527.853 kb on - strand at 527.853 kb on - strand at 527.862 kb on + strand at 527.864 kb on + strand at 527.872 kb on - strand at 527.879 kb on + strand at 527.890 kb on + strand at 527.890 kb on + strand at 527.921 kb on + strand at 527.923 kb on - strand at 527.969 kb on - strand at 527.973 kb on + strand at 528.000 kb on + strand at 528.021 kb on + strand at 528.024 kb on - strand at 528.042 kb on + strand at 528.075 kb on + strand at 528.076 kb on + strand at 528.081 kb on + strand at 528.084 kb on - strand at 528.099 kb on + strand, within nha4 at 528.102 kb on - strand, within nha4 at 528.102 kb on - strand, within nha4 at 528.140 kb on - strand, within nha4 at 528.140 kb on - strand, within nha4 at 528.140 kb on - strand, within nha4 at 528.140 kb on - strand, within nha4 at 528.140 kb on - strand, within nha4 at 528.140 kb on - strand, within nha4
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.0625 mg/ml remove 526,266 - +2.9 526,268 + -1.7 526,328 + Synpcc7942_0543 0.14 +1.2 526,358 + Synpcc7942_0543 0.19 +0.3 526,361 - Synpcc7942_0543 0.20 -1.3 526,361 - Synpcc7942_0543 0.20 -1.5 526,395 + Synpcc7942_0543 0.26 +0.3 526,403 - Synpcc7942_0543 0.27 +0.4 526,418 + Synpcc7942_0543 0.30 +0.3 526,426 - Synpcc7942_0543 0.31 +0.9 526,439 + Synpcc7942_0543 0.34 +0.0 526,439 + +0.3 526,498 - Synpcc7942_0543 0.44 +0.7 526,505 + Synpcc7942_0543 0.45 +2.1 526,505 + Synpcc7942_0543 0.45 +0.3 526,518 - Synpcc7942_0543 0.48 -1.0 526,532 - Synpcc7942_0543 0.50 +1.3 526,552 + Synpcc7942_0543 0.54 +0.7 526,565 + Synpcc7942_0543 0.56 -1.3 526,573 - Synpcc7942_0543 0.57 +1.9 526,573 - Synpcc7942_0543 0.57 -2.5 526,573 - Synpcc7942_0543 0.57 -2.3 526,588 - Synpcc7942_0543 0.60 -1.5 526,644 - Synpcc7942_0543 0.70 +2.6 526,646 - Synpcc7942_0543 0.70 -1.5 526,651 - Synpcc7942_0543 0.71 +0.0 526,689 - Synpcc7942_0543 0.78 -1.3 526,689 - Synpcc7942_0543 0.78 +1.2 526,711 + Synpcc7942_0543 0.82 -2.0 526,764 + -1.6 526,769 + +4.8 526,770 - +1.1 526,770 - +0.9 526,774 + +1.7 526,782 - -1.7 526,803 + +2.5 526,804 - +2.3 526,804 - +0.7 526,820 + -1.3 526,876 + -1.3 526,877 + +0.2 526,915 - -2.0 526,921 + Synpcc7942_0544 0.11 -1.1 526,982 + Synpcc7942_0544 0.33 -0.7 526,990 - Synpcc7942_0544 0.36 -1.7 526,997 + -0.7 526,999 - Synpcc7942_0544 0.40 -0.8 527,000 + Synpcc7942_0544 0.40 +2.3 527,002 + Synpcc7942_0544 0.41 -0.7 527,022 + Synpcc7942_0544 0.48 -0.4 527,022 + Synpcc7942_0544 0.48 -1.3 527,022 + Synpcc7942_0544 0.48 -1.7 527,022 + Synpcc7942_0544 0.48 -0.5 527,022 + Synpcc7942_0544 0.48 -0.6 527,022 + Synpcc7942_0544 0.48 -0.7 527,022 + Synpcc7942_0544 0.48 -1.1 527,027 + Synpcc7942_0544 0.50 +0.3 527,030 - Synpcc7942_0544 0.51 +0.3 527,030 - Synpcc7942_0544 0.51 -1.6 527,035 - Synpcc7942_0544 0.53 -1.5 527,035 - Synpcc7942_0544 0.53 -1.5 527,081 - Synpcc7942_0544 0.70 +0.0 527,081 - Synpcc7942_0544 0.70 -1.7 527,126 + Synpcc7942_0544 0.87 -2.5 527,135 + +4.1 527,135 - +1.9 527,135 - +1.3 527,207 - -0.2 527,207 - -0.3 527,207 - +0.3 527,207 - -2.9 527,207 - -2.0 527,207 - -1.3 527,209 + -0.0 527,209 + +0.3 527,209 + -1.4 527,209 + +1.6 527,209 + -1.3 527,209 + +1.3 527,209 + +1.0 527,209 + -0.4 527,217 - Synpcc7942_0545 0.11 +2.2 527,217 - Synpcc7942_0545 0.11 -1.0 527,246 - Synpcc7942_0545 0.15 -2.1 527,272 - Synpcc7942_0545 0.19 +0.6 527,282 + Synpcc7942_0545 0.20 -1.0 527,282 + Synpcc7942_0545 0.20 -2.0 527,290 - Synpcc7942_0545 0.21 -0.9 527,297 + Synpcc7942_0545 0.22 -3.4 527,307 + Synpcc7942_0545 0.24 -0.4 527,307 + Synpcc7942_0545 0.24 +0.3 527,307 + Synpcc7942_0545 0.24 -0.6 527,307 + Synpcc7942_0545 0.24 -0.1 527,307 + Synpcc7942_0545 0.24 +1.0 527,307 + Synpcc7942_0545 0.24 +2.3 527,313 + Synpcc7942_0545 0.25 +3.6 527,315 - Synpcc7942_0545 0.25 -0.9 527,315 - Synpcc7942_0545 0.25 -0.7 527,315 - Synpcc7942_0545 0.25 -0.3 527,315 - Synpcc7942_0545 0.25 -0.2 527,330 - Synpcc7942_0545 0.27 -1.2 527,347 + Synpcc7942_0545 0.29 +0.3 527,350 - Synpcc7942_0545 0.30 -0.7 527,355 - Synpcc7942_0545 0.31 +0.1 527,355 - Synpcc7942_0545 0.31 +1.7 527,382 + Synpcc7942_0545 0.34 -0.7 527,398 - Synpcc7942_0545 0.37 +1.2 527,441 - Synpcc7942_0545 0.43 +1.3 527,468 - Synpcc7942_0545 0.47 -2.0 527,516 + Synpcc7942_0545 0.54 -1.0 527,521 + Synpcc7942_0545 0.55 +1.5 527,527 + Synpcc7942_0545 0.55 +1.4 527,529 - Synpcc7942_0545 0.56 -1.8 527,590 + Synpcc7942_0545 0.65 +0.6 527,598 + Synpcc7942_0545 0.66 -0.9 527,598 + Synpcc7942_0545 0.66 +0.2 527,606 - Synpcc7942_0545 0.67 +2.3 527,629 + Synpcc7942_0545 0.70 -1.1 527,637 - Synpcc7942_0545 0.71 -1.7 527,653 - Synpcc7942_0545 0.74 -1.3 527,661 - Synpcc7942_0545 0.75 -2.3 527,716 - Synpcc7942_0545 0.83 -1.3 527,757 - Synpcc7942_0545 0.89 +2.0 527,767 + -0.1 527,775 - +1.4 527,784 + -0.7 527,796 - +1.3 527,811 + +2.0 527,819 - +0.7 527,845 + -0.3 527,845 + -1.3 527,845 + -0.7 527,852 - -1.0 527,853 - -0.9 527,853 - -1.3 527,862 + -1.3 527,864 + -1.3 527,872 - -1.5 527,879 + +0.3 527,890 + -0.5 527,890 + -0.7 527,921 + +1.1 527,923 - -0.0 527,969 - -2.7 527,973 + +1.6 528,000 + -0.0 528,021 + -2.3 528,024 - +1.5 528,042 + -1.9 528,075 + +2.3 528,076 + +3.1 528,081 + +0.9 528,084 - -1.8 528,099 + nha4 Synpcc7942_0546 0.10 +0.6 528,102 - nha4 Synpcc7942_0546 0.11 +1.7 528,102 - nha4 Synpcc7942_0546 0.11 +2.2 528,140 - nha4 Synpcc7942_0546 0.12 +0.3 528,140 - nha4 Synpcc7942_0546 0.12 -0.3 528,140 - nha4 Synpcc7942_0546 0.12 +2.1 528,140 - nha4 Synpcc7942_0546 0.12 +0.3 528,140 - nha4 Synpcc7942_0546 0.12 -0.0 528,140 - nha4 Synpcc7942_0546 0.12 +1.5
Or see this region's nucleotide sequence