Experiment: Carnitine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2420 and Pf1N1B4_2421 overlap by 4 nucleotides Pf1N1B4_2421 and Pf1N1B4_2422 are separated by 77 nucleotides Pf1N1B4_2422 and Pf1N1B4_2423 are separated by 437 nucleotides
Pf1N1B4_2420 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 2,745,122 to 2,746,300
_2420
Pf1N1B4_2421 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 2,746,297 to 2,747,142
_2421
Pf1N1B4_2422 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 2,747,220 to 2,748,170
_2422
Pf1N1B4_2423 - L-serine dehydratase (EC 4.3.1.17), at 2,748,608 to 2,749,984
_2423
Position (kb)
2747
2748
2749 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2746.312 kb on + strand at 2746.312 kb on + strand at 2746.313 kb on - strand at 2746.339 kb on - strand at 2746.364 kb on - strand at 2746.626 kb on - strand, within Pf1N1B4_2421 at 2746.626 kb on - strand, within Pf1N1B4_2421 at 2746.710 kb on + strand, within Pf1N1B4_2421 at 2746.775 kb on + strand, within Pf1N1B4_2421 at 2746.775 kb on + strand, within Pf1N1B4_2421 at 2746.776 kb on - strand, within Pf1N1B4_2421 at 2746.776 kb on - strand at 2746.776 kb on - strand, within Pf1N1B4_2421 at 2746.776 kb on - strand, within Pf1N1B4_2421 at 2746.969 kb on + strand, within Pf1N1B4_2421 at 2747.108 kb on + strand at 2747.109 kb on - strand at 2747.109 kb on - strand at 2747.109 kb on - strand at 2747.122 kb on + strand at 2747.123 kb on - strand at 2747.170 kb on + strand at 2747.170 kb on + strand at 2747.170 kb on + strand at 2747.265 kb on - strand at 2747.473 kb on + strand at 2747.473 kb on + strand, within Pf1N1B4_2422 at 2747.474 kb on - strand, within Pf1N1B4_2422 at 2747.474 kb on - strand, within Pf1N1B4_2422 at 2747.474 kb on - strand, within Pf1N1B4_2422 at 2747.474 kb on - strand, within Pf1N1B4_2422 at 2747.506 kb on + strand, within Pf1N1B4_2422 at 2747.507 kb on - strand, within Pf1N1B4_2422 at 2747.507 kb on - strand, within Pf1N1B4_2422 at 2747.527 kb on + strand, within Pf1N1B4_2422 at 2747.527 kb on + strand, within Pf1N1B4_2422 at 2747.527 kb on + strand, within Pf1N1B4_2422 at 2747.528 kb on - strand, within Pf1N1B4_2422 at 2747.528 kb on - strand, within Pf1N1B4_2422 at 2747.528 kb on - strand, within Pf1N1B4_2422 at 2747.528 kb on - strand, within Pf1N1B4_2422 at 2747.528 kb on - strand, within Pf1N1B4_2422 at 2747.737 kb on + strand, within Pf1N1B4_2422 at 2747.737 kb on + strand, within Pf1N1B4_2422 at 2747.738 kb on - strand, within Pf1N1B4_2422 at 2747.738 kb on - strand, within Pf1N1B4_2422 at 2747.738 kb on - strand, within Pf1N1B4_2422 at 2747.802 kb on - strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.830 kb on + strand, within Pf1N1B4_2422 at 2747.831 kb on - strand, within Pf1N1B4_2422 at 2747.849 kb on + strand, within Pf1N1B4_2422 at 2747.922 kb on - strand, within Pf1N1B4_2422 at 2747.965 kb on + strand, within Pf1N1B4_2422 at 2747.965 kb on + strand, within Pf1N1B4_2422 at 2747.965 kb on + strand, within Pf1N1B4_2422 at 2747.966 kb on - strand, within Pf1N1B4_2422 at 2747.966 kb on - strand, within Pf1N1B4_2422 at 2748.004 kb on + strand, within Pf1N1B4_2422 at 2748.005 kb on - strand, within Pf1N1B4_2422 at 2748.170 kb on + strand at 2748.226 kb on + strand at 2748.226 kb on + strand at 2748.226 kb on + strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.300 kb on + strand at 2748.300 kb on + strand at 2748.300 kb on + strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.322 kb on - strand at 2748.379 kb on + strand at 2748.380 kb on - strand at 2748.380 kb on - strand at 2748.380 kb on - strand at 2748.547 kb on - strand at 2748.758 kb on + strand, within Pf1N1B4_2423 at 2748.758 kb on + strand, within Pf1N1B4_2423 at 2748.759 kb on - strand, within Pf1N1B4_2423 at 2748.759 kb on - strand, within Pf1N1B4_2423 at 2748.759 kb on - strand, within Pf1N1B4_2423 at 2748.897 kb on + strand, within Pf1N1B4_2423 at 2748.898 kb on - strand, within Pf1N1B4_2423 at 2748.898 kb on - strand, within Pf1N1B4_2423 at 2748.898 kb on - strand, within Pf1N1B4_2423 at 2749.034 kb on + strand, within Pf1N1B4_2423 at 2749.034 kb on + strand, within Pf1N1B4_2423 at 2749.034 kb on + strand, within Pf1N1B4_2423 at 2749.034 kb on + strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.037 kb on + strand, within Pf1N1B4_2423 at 2749.037 kb on + strand, within Pf1N1B4_2423 at 2749.037 kb on + strand, within Pf1N1B4_2423 at 2749.038 kb on - strand, within Pf1N1B4_2423 at 2749.038 kb on - strand, within Pf1N1B4_2423 at 2749.038 kb on - strand, within Pf1N1B4_2423 at 2749.118 kb on + strand, within Pf1N1B4_2423
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (C) remove 2,746,312 + -1.3 2,746,312 + -0.7 2,746,313 - -2.0 2,746,339 - -3.4 2,746,364 - -0.3 2,746,626 - Pf1N1B4_2421 0.39 -2.7 2,746,626 - Pf1N1B4_2421 0.39 -2.5 2,746,710 + Pf1N1B4_2421 0.49 -1.5 2,746,775 + Pf1N1B4_2421 0.57 -2.4 2,746,775 + Pf1N1B4_2421 0.57 +0.3 2,746,776 - Pf1N1B4_2421 0.57 -1.7 2,746,776 - +0.3 2,746,776 - Pf1N1B4_2421 0.57 -1.3 2,746,776 - Pf1N1B4_2421 0.57 -0.7 2,746,969 + Pf1N1B4_2421 0.79 -1.7 2,747,108 + -0.7 2,747,109 - -1.3 2,747,109 - -2.5 2,747,109 - -1.5 2,747,122 + -4.2 2,747,123 - -1.3 2,747,170 + -0.1 2,747,170 + -2.5 2,747,170 + +0.3 2,747,265 - -1.7 2,747,473 + -1.3 2,747,473 + Pf1N1B4_2422 0.27 -2.0 2,747,474 - Pf1N1B4_2422 0.27 -0.3 2,747,474 - Pf1N1B4_2422 0.27 -1.7 2,747,474 - Pf1N1B4_2422 0.27 -1.7 2,747,474 - Pf1N1B4_2422 0.27 -3.0 2,747,506 + Pf1N1B4_2422 0.30 +1.3 2,747,507 - Pf1N1B4_2422 0.30 -1.3 2,747,507 - Pf1N1B4_2422 0.30 -2.7 2,747,527 + Pf1N1B4_2422 0.32 -2.0 2,747,527 + Pf1N1B4_2422 0.32 -0.7 2,747,527 + Pf1N1B4_2422 0.32 -1.3 2,747,528 - Pf1N1B4_2422 0.32 -0.7 2,747,528 - Pf1N1B4_2422 0.32 -2.7 2,747,528 - Pf1N1B4_2422 0.32 -2.5 2,747,528 - Pf1N1B4_2422 0.32 -4.2 2,747,528 - Pf1N1B4_2422 0.32 +0.3 2,747,737 + Pf1N1B4_2422 0.54 -1.3 2,747,737 + Pf1N1B4_2422 0.54 -0.7 2,747,738 - Pf1N1B4_2422 0.54 -2.0 2,747,738 - Pf1N1B4_2422 0.54 -3.2 2,747,738 - Pf1N1B4_2422 0.54 +0.3 2,747,802 - Pf1N1B4_2422 0.61 -0.7 2,747,803 + Pf1N1B4_2422 0.61 -0.7 2,747,803 + Pf1N1B4_2422 0.61 -1.3 2,747,803 + Pf1N1B4_2422 0.61 -1.3 2,747,803 + Pf1N1B4_2422 0.61 -2.7 2,747,803 + Pf1N1B4_2422 0.61 -2.7 2,747,803 + Pf1N1B4_2422 0.61 -0.7 2,747,803 + Pf1N1B4_2422 0.61 -1.7 2,747,804 - Pf1N1B4_2422 0.61 -1.7 2,747,804 - Pf1N1B4_2422 0.61 -3.5 2,747,804 - Pf1N1B4_2422 0.61 -0.7 2,747,804 - Pf1N1B4_2422 0.61 -0.7 2,747,804 - Pf1N1B4_2422 0.61 -2.5 2,747,804 - Pf1N1B4_2422 0.61 -0.7 2,747,830 + Pf1N1B4_2422 0.64 -2.0 2,747,831 - Pf1N1B4_2422 0.64 -2.5 2,747,849 + Pf1N1B4_2422 0.66 -2.7 2,747,922 - Pf1N1B4_2422 0.74 -2.7 2,747,965 + Pf1N1B4_2422 0.78 +0.3 2,747,965 + Pf1N1B4_2422 0.78 +0.3 2,747,965 + Pf1N1B4_2422 0.78 -0.7 2,747,966 - Pf1N1B4_2422 0.78 -0.7 2,747,966 - Pf1N1B4_2422 0.78 -3.0 2,748,004 + Pf1N1B4_2422 0.82 +0.3 2,748,005 - Pf1N1B4_2422 0.83 -2.3 2,748,170 + -1.3 2,748,226 + -0.3 2,748,226 + -2.5 2,748,226 + -0.9 2,748,227 - -2.0 2,748,227 - -2.1 2,748,227 - -2.7 2,748,227 - -2.0 2,748,227 - -3.8 2,748,227 - -1.7 2,748,227 - -2.0 2,748,227 - -2.8 2,748,227 - -2.5 2,748,300 + -2.5 2,748,300 + -3.0 2,748,300 + -2.7 2,748,301 - -2.0 2,748,301 - -1.7 2,748,301 - -2.3 2,748,301 - -1.7 2,748,301 - -2.6 2,748,301 - -0.3 2,748,301 - -2.7 2,748,301 - -2.5 2,748,301 - -2.0 2,748,301 - -1.5 2,748,301 - +0.9 2,748,301 - -1.3 2,748,301 - -2.0 2,748,322 - -1.0 2,748,379 + -1.7 2,748,380 - -3.3 2,748,380 - -1.4 2,748,380 - -0.7 2,748,547 - -2.3 2,748,758 + Pf1N1B4_2423 0.11 -0.7 2,748,758 + Pf1N1B4_2423 0.11 +1.3 2,748,759 - Pf1N1B4_2423 0.11 -0.2 2,748,759 - Pf1N1B4_2423 0.11 -0.3 2,748,759 - Pf1N1B4_2423 0.11 -1.6 2,748,897 + Pf1N1B4_2423 0.21 -0.1 2,748,898 - Pf1N1B4_2423 0.21 -1.7 2,748,898 - Pf1N1B4_2423 0.21 -0.6 2,748,898 - Pf1N1B4_2423 0.21 -1.3 2,749,034 + Pf1N1B4_2423 0.31 -1.7 2,749,034 + Pf1N1B4_2423 0.31 -1.3 2,749,034 + Pf1N1B4_2423 0.31 -0.7 2,749,034 + Pf1N1B4_2423 0.31 -1.0 2,749,035 - Pf1N1B4_2423 0.31 -2.5 2,749,035 - Pf1N1B4_2423 0.31 -2.3 2,749,035 - Pf1N1B4_2423 0.31 +1.3 2,749,035 - Pf1N1B4_2423 0.31 -0.7 2,749,035 - Pf1N1B4_2423 0.31 -0.1 2,749,035 - Pf1N1B4_2423 0.31 +1.0 2,749,035 - Pf1N1B4_2423 0.31 -0.0 2,749,035 - Pf1N1B4_2423 0.31 -0.0 2,749,035 - Pf1N1B4_2423 0.31 -1.3 2,749,037 + Pf1N1B4_2423 0.31 +0.0 2,749,037 + Pf1N1B4_2423 0.31 -2.5 2,749,037 + Pf1N1B4_2423 0.31 -0.7 2,749,038 - Pf1N1B4_2423 0.31 -1.3 2,749,038 - Pf1N1B4_2423 0.31 -1.3 2,749,038 - Pf1N1B4_2423 0.31 -1.1 2,749,118 + Pf1N1B4_2423 0.37 -2.3
Or see this region's nucleotide sequence