Experiment: No stressor control+ 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1543 and Dsui_1544 are separated by 73 nucleotides Dsui_1544 and Dsui_1545 are separated by 249 nucleotides Dsui_1545 and Dsui_1546 are separated by 36 nucleotides
Dsui_1543: Dsui_1543 - tRNA/rRNA cytosine-C5-methylase, at 1,603,433 to 1,604,761
_1543
Dsui_1544: Dsui_1544 - Protein of unknown function (DUF3108), at 1,604,835 to 1,605,572
_1544
Dsui_1545: Dsui_1545 - phosphoribosylglycinamide formyltransferase, formyltetrahydrofolate-dependent, at 1,605,822 to 1,606,478
_1545
Dsui_1546: Dsui_1546 - hypothetical protein, at 1,606,515 to 1,607,267
_1546
Position (kb)
1604
1605
1606 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1603.892 kb on - strand, within Dsui_1543 at 1603.894 kb on + strand at 1603.894 kb on + strand at 1603.923 kb on - strand, within Dsui_1543 at 1604.095 kb on + strand at 1604.254 kb on + strand at 1604.280 kb on - strand, within Dsui_1543 at 1604.307 kb on + strand at 1604.378 kb on + strand, within Dsui_1543 at 1604.380 kb on - strand, within Dsui_1543 at 1604.568 kb on - strand, within Dsui_1543 at 1605.085 kb on + strand at 1605.113 kb on - strand, within Dsui_1544 at 1605.120 kb on - strand, within Dsui_1544 at 1605.303 kb on + strand at 1605.397 kb on + strand, within Dsui_1544 at 1605.751 kb on - strand at 1605.820 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction No stressor control+ 10 mM ClO4 and 20 mM lactate remove 1,603,892 - Dsui_1543 0.35 +0.1 1,603,894 + +1.6 1,603,894 + +0.8 1,603,923 - Dsui_1543 0.37 +1.9 1,604,095 + +0.6 1,604,254 + -0.2 1,604,280 - Dsui_1543 0.64 +0.7 1,604,307 + +3.1 1,604,378 + Dsui_1543 0.71 -0.7 1,604,380 - Dsui_1543 0.71 +0.5 1,604,568 - Dsui_1543 0.85 -1.9 1,605,085 + -1.9 1,605,113 - Dsui_1544 0.38 -1.5 1,605,120 - Dsui_1544 0.39 -1.8 1,605,303 + -4.7 1,605,397 + Dsui_1544 0.76 -3.2 1,605,751 - -2.6 1,605,820 + -1.3
Or see this region's nucleotide sequence