Experiment: ICP1_phage_0.23_MOI_rep1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_16025 and CSW01_16030 are separated by 20 nucleotides CSW01_16030 and CSW01_16035 are separated by 424 nucleotides CSW01_16035 and CSW01_16040 are separated by 200 nucleotides CSW01_16040 and CSW01_16045 are separated by 10 nucleotides CSW01_16045 and CSW01_16050 overlap by 85 nucleotides
CSW01_16025: CSW01_16025 - hypothetical protein, at 366,288 to 366,481
_16025
CSW01_16030: CSW01_16030 - DUF3709 domain-containing protein, at 366,502 to 366,735
_16030
CSW01_16035: CSW01_16035 - DUF645 domain-containing protein, at 367,160 to 367,340
_16035
CSW01_16040: CSW01_16040 - type II toxin-antitoxin system RelE/ParE family toxin, at 367,541 to 367,894
_16040
CSW01_16045: CSW01_16045 - transcriptional regulator, at 367,905 to 368,222
_16045
CSW01_16050: CSW01_16050 - DUF3265 domain-containing protein, at 368,138 to 368,329
_16050
Position (kb) 367
368 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 366.449 kb on - strand, within CSW01_16025 at 366.504 kb on - strand at 366.506 kb on - strand at 366.547 kb on - strand, within CSW01_16030 at 366.547 kb on - strand, within CSW01_16030 at 366.557 kb on - strand, within CSW01_16030 at 366.783 kb on - strand at 366.783 kb on - strand at 366.826 kb on - strand at 367.241 kb on + strand, within CSW01_16035 at 367.241 kb on + strand, within CSW01_16035 at 367.241 kb on + strand, within CSW01_16035 at 367.249 kb on - strand, within CSW01_16035 at 367.249 kb on - strand, within CSW01_16035 at 367.249 kb on - strand, within CSW01_16035 at 367.249 kb on - strand, within CSW01_16035 at 367.479 kb on - strand at 367.649 kb on - strand, within CSW01_16040 at 367.668 kb on + strand, within CSW01_16040 at 367.668 kb on + strand, within CSW01_16040 at 367.727 kb on + strand, within CSW01_16040 at 367.775 kb on - strand, within CSW01_16040
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_0.23_MOI_rep1 remove 366,449 - CSW01_16025 0.83 +0.3 366,504 - -0.1 366,506 - -2.4 366,547 - CSW01_16030 0.19 +0.8 366,547 - CSW01_16030 0.19 -4.1 366,557 - CSW01_16030 0.24 -2.9 366,783 - -1.5 366,783 - -0.4 366,826 - -1.7 367,241 + CSW01_16035 0.45 -0.6 367,241 + CSW01_16035 0.45 -0.9 367,241 + CSW01_16035 0.45 -3.7 367,249 - CSW01_16035 0.49 -2.9 367,249 - CSW01_16035 0.49 -1.6 367,249 - CSW01_16035 0.49 +0.4 367,249 - CSW01_16035 0.49 -1.7 367,479 - +0.9 367,649 - CSW01_16040 0.31 +0.1 367,668 + CSW01_16040 0.36 +0.2 367,668 + CSW01_16040 0.36 -0.1 367,727 + CSW01_16040 0.53 -1.2 367,775 - CSW01_16040 0.66 -0.4
Or see this region's nucleotide sequence