Experiment: ICP1_phage_0.23_MOI_rep1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_10255 and CSW01_10260 overlap by 4 nucleotides CSW01_10260 and CSW01_10265 are separated by 0 nucleotides CSW01_10265 and CSW01_10270 overlap by 4 nucleotides CSW01_10270 and CSW01_10275 overlap by 4 nucleotides CSW01_10275 and CSW01_10280 overlap by 1 nucleotides
CSW01_10255: CSW01_10255 - cytochrome c-type biogenesis protein CcmH, at 2,206,254 to 2,206,730
_10255
CSW01_10260: CSW01_10260 - thiol:disulfide interchange protein DsbE, at 2,206,727 to 2,207,281
_10260
CSW01_10265: CSW01_10265 - c-type cytochrome biogenesis protein CcmF, at 2,207,282 to 2,209,237
_10265
CSW01_10270: CSW01_10270 - cytochrome c-type biogenesis protein CcmE, at 2,209,234 to 2,209,716
_10270
CSW01_10275: CSW01_10275 - heme exporter protein CcmD, at 2,209,713 to 2,209,919
_10275
CSW01_10280: CSW01_10280 - heme exporter protein C, at 2,209,919 to 2,210,671
_10280
Position (kb)
2207
2208
2209
2210 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2206.474 kb on + strand, within CSW01_10255 at 2206.637 kb on + strand, within CSW01_10255 at 2206.645 kb on - strand, within CSW01_10255 at 2206.645 kb on - strand, within CSW01_10255 at 2206.847 kb on + strand, within CSW01_10260 at 2206.865 kb on - strand, within CSW01_10260 at 2206.873 kb on + strand, within CSW01_10260 at 2206.915 kb on + strand, within CSW01_10260 at 2206.915 kb on + strand, within CSW01_10260 at 2206.923 kb on - strand, within CSW01_10260 at 2206.923 kb on - strand, within CSW01_10260 at 2207.288 kb on + strand at 2207.296 kb on - strand at 2207.353 kb on - strand at 2207.399 kb on - strand at 2207.412 kb on + strand at 2207.412 kb on + strand at 2207.420 kb on - strand at 2207.691 kb on + strand, within CSW01_10265 at 2207.846 kb on - strand, within CSW01_10265 at 2207.846 kb on - strand, within CSW01_10265 at 2207.959 kb on + strand, within CSW01_10265 at 2207.964 kb on + strand, within CSW01_10265 at 2207.964 kb on + strand, within CSW01_10265 at 2207.964 kb on + strand, within CSW01_10265 at 2207.972 kb on - strand, within CSW01_10265 at 2207.972 kb on - strand, within CSW01_10265 at 2207.972 kb on - strand, within CSW01_10265 at 2207.972 kb on - strand, within CSW01_10265 at 2207.972 kb on - strand, within CSW01_10265 at 2207.972 kb on - strand, within CSW01_10265 at 2207.972 kb on - strand, within CSW01_10265 at 2207.972 kb on - strand, within CSW01_10265 at 2207.972 kb on - strand, within CSW01_10265 at 2207.972 kb on - strand, within CSW01_10265 at 2208.010 kb on - strand, within CSW01_10265 at 2208.418 kb on + strand, within CSW01_10265 at 2208.445 kb on + strand, within CSW01_10265 at 2208.453 kb on - strand, within CSW01_10265 at 2208.561 kb on - strand, within CSW01_10265 at 2208.603 kb on + strand, within CSW01_10265 at 2208.611 kb on - strand, within CSW01_10265 at 2208.618 kb on - strand, within CSW01_10265 at 2208.973 kb on + strand, within CSW01_10265 at 2208.986 kb on - strand, within CSW01_10265 at 2209.138 kb on - strand at 2209.275 kb on - strand at 2209.332 kb on - strand, within CSW01_10270 at 2209.399 kb on + strand, within CSW01_10270 at 2209.399 kb on + strand, within CSW01_10270 at 2209.402 kb on - strand, within CSW01_10270 at 2209.442 kb on - strand, within CSW01_10270 at 2209.454 kb on - strand, within CSW01_10270 at 2209.607 kb on - strand, within CSW01_10270 at 2209.644 kb on + strand, within CSW01_10270 at 2209.652 kb on - strand, within CSW01_10270 at 2209.960 kb on + strand at 2209.968 kb on - strand at 2210.135 kb on - strand, within CSW01_10280 at 2210.137 kb on + strand, within CSW01_10280 at 2210.150 kb on - strand, within CSW01_10280 at 2210.211 kb on + strand, within CSW01_10280
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_0.23_MOI_rep1 remove 2,206,474 + CSW01_10255 0.46 -2.3 2,206,637 + CSW01_10255 0.80 -2.3 2,206,645 - CSW01_10255 0.82 -1.6 2,206,645 - CSW01_10255 0.82 -2.5 2,206,847 + CSW01_10260 0.22 +0.4 2,206,865 - CSW01_10260 0.25 -2.0 2,206,873 + CSW01_10260 0.26 -3.6 2,206,915 + CSW01_10260 0.34 -3.7 2,206,915 + CSW01_10260 0.34 -1.7 2,206,923 - CSW01_10260 0.35 -2.2 2,206,923 - CSW01_10260 0.35 -1.9 2,207,288 + +1.5 2,207,296 - +0.6 2,207,353 - -2.7 2,207,399 - -2.5 2,207,412 + +0.8 2,207,412 + +0.2 2,207,420 - -3.2 2,207,691 + CSW01_10265 0.21 -1.9 2,207,846 - CSW01_10265 0.29 -2.1 2,207,846 - CSW01_10265 0.29 +3.0 2,207,959 + CSW01_10265 0.35 -2.0 2,207,964 + CSW01_10265 0.35 -2.0 2,207,964 + CSW01_10265 0.35 -1.9 2,207,964 + CSW01_10265 0.35 -1.7 2,207,972 - CSW01_10265 0.35 -0.0 2,207,972 - CSW01_10265 0.35 -2.4 2,207,972 - CSW01_10265 0.35 -3.0 2,207,972 - CSW01_10265 0.35 -1.5 2,207,972 - CSW01_10265 0.35 +1.6 2,207,972 - CSW01_10265 0.35 -2.1 2,207,972 - CSW01_10265 0.35 -0.4 2,207,972 - CSW01_10265 0.35 -2.5 2,207,972 - CSW01_10265 0.35 -2.3 2,207,972 - CSW01_10265 0.35 -1.9 2,208,010 - CSW01_10265 0.37 -2.3 2,208,418 + CSW01_10265 0.58 -0.0 2,208,445 + CSW01_10265 0.59 -2.1 2,208,453 - CSW01_10265 0.60 -3.0 2,208,561 - CSW01_10265 0.65 -0.8 2,208,603 + CSW01_10265 0.68 -2.0 2,208,611 - CSW01_10265 0.68 +0.5 2,208,618 - CSW01_10265 0.68 +0.7 2,208,973 + CSW01_10265 0.86 +0.5 2,208,986 - CSW01_10265 0.87 -1.0 2,209,138 - -2.1 2,209,275 - -1.1 2,209,332 - CSW01_10270 0.20 -2.7 2,209,399 + CSW01_10270 0.34 -1.4 2,209,399 + CSW01_10270 0.34 -0.3 2,209,402 - CSW01_10270 0.35 -1.4 2,209,442 - CSW01_10270 0.43 -0.5 2,209,454 - CSW01_10270 0.46 -2.8 2,209,607 - CSW01_10270 0.77 -1.9 2,209,644 + CSW01_10270 0.85 -1.1 2,209,652 - CSW01_10270 0.87 -3.3 2,209,960 + -3.1 2,209,968 - +2.5 2,210,135 - CSW01_10280 0.29 +0.2 2,210,137 + CSW01_10280 0.29 -2.4 2,210,150 - CSW01_10280 0.31 -1.5 2,210,211 + CSW01_10280 0.39 -3.8
Or see this region's nucleotide sequence